DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.

Sand flies include a group of insects that are of medical importance and that vary in geographic distribution, ecology, and pathogen transmission. Approximately 163 species of sand flies have been reported in Colombia. Surveillance of the presence of sand fly species and the actualization of species...

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Autores principales:	María Angélica Contreras Gutiérrez, Rafael J Vivero, Iván D Vélez, Charles H Porter, Sandra Uribe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43281125a2984fddb96d987538601b08
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spelling	oai:doaj.org-article:43281125a2984fddb96d987538601b082021-11-18T08:37:40ZDNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.1932-620310.1371/journal.pone.0085496https://doaj.org/article/43281125a2984fddb96d987538601b082014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454877/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Sand flies include a group of insects that are of medical importance and that vary in geographic distribution, ecology, and pathogen transmission. Approximately 163 species of sand flies have been reported in Colombia. Surveillance of the presence of sand fly species and the actualization of species distribution are important for predicting risks for and monitoring the expansion of diseases which sand flies can transmit. Currently, the identification of phlebotomine sand flies is based on morphological characters. However, morphological identification requires considerable skills and taxonomic expertise. In addition, significant morphological similarity between some species, especially among females, may cause difficulties during the identification process. DNA-based approaches have become increasingly useful and promising tools for estimating sand fly diversity and for ensuring the rapid and accurate identification of species. A partial sequence of the mitochondrial cytochrome oxidase gene subunit I (COI) is currently being used to differentiate species in different animal taxa, including insects, and it is referred as a barcoding sequence. The present study explored the utility of the DNA barcode approach for the identification of phlebotomine sand flies in Colombia. We sequenced 700 bp of the COI gene from 36 species collected from different geographic localities. The COI barcode sequence divergence within a single species was <2% in most cases, whereas this divergence ranged from 9% to 26.6% among different species. These results indicated that the barcoding gene correctly discriminated among the previously morphologically identified species with an efficacy of nearly 100%. Analyses of the generated sequences indicated that the observed species groupings were consistent with the morphological identifications. In conclusion, the barcoding gene was useful for species discrimination in sand flies from Colombia.María Angélica Contreras GutiérrezRafael J ViveroIván D VélezCharles H PorterSandra UribePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85496 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María Angélica Contreras Gutiérrez Rafael J Vivero Iván D Vélez Charles H Porter Sandra Uribe DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.
description	Sand flies include a group of insects that are of medical importance and that vary in geographic distribution, ecology, and pathogen transmission. Approximately 163 species of sand flies have been reported in Colombia. Surveillance of the presence of sand fly species and the actualization of species distribution are important for predicting risks for and monitoring the expansion of diseases which sand flies can transmit. Currently, the identification of phlebotomine sand flies is based on morphological characters. However, morphological identification requires considerable skills and taxonomic expertise. In addition, significant morphological similarity between some species, especially among females, may cause difficulties during the identification process. DNA-based approaches have become increasingly useful and promising tools for estimating sand fly diversity and for ensuring the rapid and accurate identification of species. A partial sequence of the mitochondrial cytochrome oxidase gene subunit I (COI) is currently being used to differentiate species in different animal taxa, including insects, and it is referred as a barcoding sequence. The present study explored the utility of the DNA barcode approach for the identification of phlebotomine sand flies in Colombia. We sequenced 700 bp of the COI gene from 36 species collected from different geographic localities. The COI barcode sequence divergence within a single species was <2% in most cases, whereas this divergence ranged from 9% to 26.6% among different species. These results indicated that the barcoding gene correctly discriminated among the previously morphologically identified species with an efficacy of nearly 100%. Analyses of the generated sequences indicated that the observed species groupings were consistent with the morphological identifications. In conclusion, the barcoding gene was useful for species discrimination in sand flies from Colombia.
format	article
author	María Angélica Contreras Gutiérrez Rafael J Vivero Iván D Vélez Charles H Porter Sandra Uribe
author_facet	María Angélica Contreras Gutiérrez Rafael J Vivero Iván D Vélez Charles H Porter Sandra Uribe
author_sort	María Angélica Contreras Gutiérrez
title	DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.
title_short	DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.
title_full	DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.
title_fullStr	DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.
title_full_unstemmed	DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.
title_sort	dna barcoding for the identification of sand fly species (diptera, psychodidae, phlebotominae) in colombia.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/43281125a2984fddb96d987538601b08
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DNA barcoding for the identification of sand fly species (Diptera, Psychodidae, Phlebotominae) in Colombia.

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