Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>

ABSTRACT The mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant Gram-negative bacteria. To date, six slightly different variants...

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Autores principales:	Wenjuan Yin, Hui Li, Yingbo Shen, Zhihai Liu, Shaolin Wang, Zhangqi Shen, Rong Zhang, Timothy R. Walsh, Jianzhong Shen, Yang Wang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Aeromonas colistin resistance Enterobacteriaceae mcr-3 public health Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/432f19e4c7c24b39ad141f4b49b5e16c
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spelling	oai:doaj.org-article:432f19e4c7c24b39ad141f4b49b5e16c2021-11-15T15:51:30ZNovel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>10.1128/mBio.00543-172150-7511https://doaj.org/article/432f19e4c7c24b39ad141f4b49b5e16c2017-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00543-17https://doaj.org/toc/2150-7511ABSTRACT The mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant Gram-negative bacteria. To date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the GenBank database. Here, we characterized a third mobile colistin resistance gene, mcr-3. The gene coexisted with 18 additional resistance determinants in the 261-kb IncHI2-type plasmid pWJ1 from porcine Escherichia coli. mcr-3 showed 45.0% and 47.0% nucleotide sequence identity to mcr-1 and mcr-2, respectively, while the deduced amino acid sequence of MCR-3 showed 99.8 to 100% and 75.6 to 94.8% identity to phosphoethanolamine transferases found in other Enterobacteriaceae species and in 10 Aeromonas species, respectively. pWJ1 was mobilized to an E. coli recipient by conjugation and contained a plasmid backbone similar to those of other mcr-1-carrying plasmids, such as pHNSHP45-2 from the original mcr-1-harboring E. coli strain. Moreover, a truncated transposon element, TnAs2, which was characterized only in Aeromonas salmonicida, was located upstream of mcr-3 in pWJ1. This ΔTnAs2-mcr-3 element was also identified in a shotgun genome sequence of a porcine E. coli isolate from Malaysia, a human Klebsiella pneumoniae isolate from Thailand, and a human Salmonella enterica serovar Typhimurium isolate from the United States. These results suggest the likelihood of a wide dissemination of the novel mobile colistin resistance gene mcr-3 among Enterobacteriaceae and aeromonads; the latter may act as a potential reservoir for mcr-3. IMPORTANCE The emergence of the plasmid-mediated colistin resistance gene mcr-1 has attracted substantial attention worldwide. Here, we examined a colistin-resistant Escherichia coli isolate that was negative for both mcr-1 and mcr-2 and discovered a novel mobile colistin resistance gene, mcr-3. The amino acid sequence of MCR-3 aligned closely with phosphoethanolamine transferases from Enterobacteriaceae and Aeromonas species originating from both clinical infections and environmental samples collected in 12 countries on four continents. Due to the ubiquitous profile of aeromonads in the environment and the potential transfer of mcr-3 between Enterobacteriaceae and Aeromonas species, the wide spread of mcr-3 may be largely underestimated. As colistin has been and still is widely used in veterinary medicine and used at increasing frequencies in human medicine, the continuous monitoring of mobile colistin resistance determinants in colistin-resistant Gram-negative bacteria is imperative for understanding and tackling the dissemination of mcr genes in both the agricultural and health care sectors.Wenjuan YinHui LiYingbo ShenZhihai LiuShaolin WangZhangqi ShenRong ZhangTimothy R. WalshJianzhong ShenYang WangAmerican Society for MicrobiologyarticleAeromonascolistin resistanceEnterobacteriaceaemcr-3public healthMicrobiologyQR1-502ENmBio, Vol 8, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aeromonas colistin resistance Enterobacteriaceae mcr-3 public health Microbiology QR1-502
spellingShingle	Aeromonas colistin resistance Enterobacteriaceae mcr-3 public health Microbiology QR1-502 Wenjuan Yin Hui Li Yingbo Shen Zhihai Liu Shaolin Wang Zhangqi Shen Rong Zhang Timothy R. Walsh Jianzhong Shen Yang Wang Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>
description	ABSTRACT The mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant Gram-negative bacteria. To date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the GenBank database. Here, we characterized a third mobile colistin resistance gene, mcr-3. The gene coexisted with 18 additional resistance determinants in the 261-kb IncHI2-type plasmid pWJ1 from porcine Escherichia coli. mcr-3 showed 45.0% and 47.0% nucleotide sequence identity to mcr-1 and mcr-2, respectively, while the deduced amino acid sequence of MCR-3 showed 99.8 to 100% and 75.6 to 94.8% identity to phosphoethanolamine transferases found in other Enterobacteriaceae species and in 10 Aeromonas species, respectively. pWJ1 was mobilized to an E. coli recipient by conjugation and contained a plasmid backbone similar to those of other mcr-1-carrying plasmids, such as pHNSHP45-2 from the original mcr-1-harboring E. coli strain. Moreover, a truncated transposon element, TnAs2, which was characterized only in Aeromonas salmonicida, was located upstream of mcr-3 in pWJ1. This ΔTnAs2-mcr-3 element was also identified in a shotgun genome sequence of a porcine E. coli isolate from Malaysia, a human Klebsiella pneumoniae isolate from Thailand, and a human Salmonella enterica serovar Typhimurium isolate from the United States. These results suggest the likelihood of a wide dissemination of the novel mobile colistin resistance gene mcr-3 among Enterobacteriaceae and aeromonads; the latter may act as a potential reservoir for mcr-3. IMPORTANCE The emergence of the plasmid-mediated colistin resistance gene mcr-1 has attracted substantial attention worldwide. Here, we examined a colistin-resistant Escherichia coli isolate that was negative for both mcr-1 and mcr-2 and discovered a novel mobile colistin resistance gene, mcr-3. The amino acid sequence of MCR-3 aligned closely with phosphoethanolamine transferases from Enterobacteriaceae and Aeromonas species originating from both clinical infections and environmental samples collected in 12 countries on four continents. Due to the ubiquitous profile of aeromonads in the environment and the potential transfer of mcr-3 between Enterobacteriaceae and Aeromonas species, the wide spread of mcr-3 may be largely underestimated. As colistin has been and still is widely used in veterinary medicine and used at increasing frequencies in human medicine, the continuous monitoring of mobile colistin resistance determinants in colistin-resistant Gram-negative bacteria is imperative for understanding and tackling the dissemination of mcr genes in both the agricultural and health care sectors.
format	article
author	Wenjuan Yin Hui Li Yingbo Shen Zhihai Liu Shaolin Wang Zhangqi Shen Rong Zhang Timothy R. Walsh Jianzhong Shen Yang Wang
author_facet	Wenjuan Yin Hui Li Yingbo Shen Zhihai Liu Shaolin Wang Zhangqi Shen Rong Zhang Timothy R. Walsh Jianzhong Shen Yang Wang
author_sort	Wenjuan Yin
title	Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>
title_short	Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>
title_full	Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>
title_fullStr	Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>
title_full_unstemmed	Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>
title_sort	novel plasmid-mediated colistin resistance gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">escherichia coli</italic>
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/432f19e4c7c24b39ad141f4b49b5e16c
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Novel Plasmid-Mediated Colistin Resistance Gene <italic toggle="yes">mcr-3</italic> in <italic toggle="yes">Escherichia coli</italic>

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