Fast three-color single-molecule FRET using statistical inference
Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.
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Nature Portfolio
2020
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oai:doaj.org-article:43303b5b95fe4c4c8636c35d24a12b282021-12-02T16:10:47ZFast three-color single-molecule FRET using statistical inference10.1038/s41467-020-17149-w2041-1723https://doaj.org/article/43303b5b95fe4c4c8636c35d24a12b282020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17149-whttps://doaj.org/toc/2041-1723Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.Janghyun YooJae-Yeol KimJohn M. LouisIrina V. GopichHoi Sung ChungNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020) |
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Science Q Janghyun Yoo Jae-Yeol Kim John M. Louis Irina V. Gopich Hoi Sung Chung Fast three-color single-molecule FRET using statistical inference |
description |
Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation. |
format |
article |
author |
Janghyun Yoo Jae-Yeol Kim John M. Louis Irina V. Gopich Hoi Sung Chung |
author_facet |
Janghyun Yoo Jae-Yeol Kim John M. Louis Irina V. Gopich Hoi Sung Chung |
author_sort |
Janghyun Yoo |
title |
Fast three-color single-molecule FRET using statistical inference |
title_short |
Fast three-color single-molecule FRET using statistical inference |
title_full |
Fast three-color single-molecule FRET using statistical inference |
title_fullStr |
Fast three-color single-molecule FRET using statistical inference |
title_full_unstemmed |
Fast three-color single-molecule FRET using statistical inference |
title_sort |
fast three-color single-molecule fret using statistical inference |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/43303b5b95fe4c4c8636c35d24a12b28 |
work_keys_str_mv |
AT janghyunyoo fastthreecolorsinglemoleculefretusingstatisticalinference AT jaeyeolkim fastthreecolorsinglemoleculefretusingstatisticalinference AT johnmlouis fastthreecolorsinglemoleculefretusingstatisticalinference AT irinavgopich fastthreecolorsinglemoleculefretusingstatisticalinference AT hoisungchung fastthreecolorsinglemoleculefretusingstatisticalinference |
_version_ |
1718384406169649152 |