Fast three-color single-molecule FRET using statistical inference

Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.

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Autores principales: Janghyun Yoo, Jae-Yeol Kim, John M. Louis, Irina V. Gopich, Hoi Sung Chung
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/43303b5b95fe4c4c8636c35d24a12b28
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spelling oai:doaj.org-article:43303b5b95fe4c4c8636c35d24a12b282021-12-02T16:10:47ZFast three-color single-molecule FRET using statistical inference10.1038/s41467-020-17149-w2041-1723https://doaj.org/article/43303b5b95fe4c4c8636c35d24a12b282020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17149-whttps://doaj.org/toc/2041-1723Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.Janghyun YooJae-Yeol KimJohn M. LouisIrina V. GopichHoi Sung ChungNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Janghyun Yoo
Jae-Yeol Kim
John M. Louis
Irina V. Gopich
Hoi Sung Chung
Fast three-color single-molecule FRET using statistical inference
description Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.
format article
author Janghyun Yoo
Jae-Yeol Kim
John M. Louis
Irina V. Gopich
Hoi Sung Chung
author_facet Janghyun Yoo
Jae-Yeol Kim
John M. Louis
Irina V. Gopich
Hoi Sung Chung
author_sort Janghyun Yoo
title Fast three-color single-molecule FRET using statistical inference
title_short Fast three-color single-molecule FRET using statistical inference
title_full Fast three-color single-molecule FRET using statistical inference
title_fullStr Fast three-color single-molecule FRET using statistical inference
title_full_unstemmed Fast three-color single-molecule FRET using statistical inference
title_sort fast three-color single-molecule fret using statistical inference
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/43303b5b95fe4c4c8636c35d24a12b28
work_keys_str_mv AT janghyunyoo fastthreecolorsinglemoleculefretusingstatisticalinference
AT jaeyeolkim fastthreecolorsinglemoleculefretusingstatisticalinference
AT johnmlouis fastthreecolorsinglemoleculefretusingstatisticalinference
AT irinavgopich fastthreecolorsinglemoleculefretusingstatisticalinference
AT hoisungchung fastthreecolorsinglemoleculefretusingstatisticalinference
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