A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting
Abstract We report the development of a lab-on-a-chip system, that facilitates coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C), for investigating drug resistance in K562 and CCRF-CEM leukemia cells without (immuno) labeling. Two IM-C units were placed upstream and downst...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/4330e938ceb241da919d8e16d7e7f523 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:4330e938ceb241da919d8e16d7e7f523 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:4330e938ceb241da919d8e16d7e7f5232021-12-02T17:44:55ZA microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting10.1038/s41598-021-92647-52045-2322https://doaj.org/article/4330e938ceb241da919d8e16d7e7f5232021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92647-5https://doaj.org/toc/2045-2322Abstract We report the development of a lab-on-a-chip system, that facilitates coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C), for investigating drug resistance in K562 and CCRF-CEM leukemia cells without (immuno) labeling. Two IM-C units were placed upstream and downstream of the DEP-D unit for enumeration, respectively, before and after the cells were treated in DEP-D unit, where the difference in cell count gave the total number of trapped cells based on their DEP characteristics. Conductivity of the running buffer was matched the conductivity of cytoplasm of wild type K562 and CCRF-CEM cells. Results showed that DEP responses of drug resistant and wild type K562 cells were statistically discriminative (at p = 0.05 level) at 200 mS/m buffer conductivity and at 8.6 MHz working frequency of DEP-D unit. For CCRF-CEM cells, conductivity and frequency values were 160 mS/m and 6.2 MHz, respectively. Our approach enabled discrimination of resistant cells in a group by setting up a threshold provided by the conductivity of running buffer. Subsequent selection of drug resistant cells can be applied to investigate variations in gene expressions and occurrence of mutations related to drug resistance.Yağmur Demircan YalçınTaylan Berkin TöralSertan SukasEnder YıldırımÖzge ZorluUfuk GündüzHaluk KülahNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yağmur Demircan Yalçın Taylan Berkin Töral Sertan Sukas Ender Yıldırım Özge Zorlu Ufuk Gündüz Haluk Külah A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| description |
Abstract We report the development of a lab-on-a-chip system, that facilitates coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C), for investigating drug resistance in K562 and CCRF-CEM leukemia cells without (immuno) labeling. Two IM-C units were placed upstream and downstream of the DEP-D unit for enumeration, respectively, before and after the cells were treated in DEP-D unit, where the difference in cell count gave the total number of trapped cells based on their DEP characteristics. Conductivity of the running buffer was matched the conductivity of cytoplasm of wild type K562 and CCRF-CEM cells. Results showed that DEP responses of drug resistant and wild type K562 cells were statistically discriminative (at p = 0.05 level) at 200 mS/m buffer conductivity and at 8.6 MHz working frequency of DEP-D unit. For CCRF-CEM cells, conductivity and frequency values were 160 mS/m and 6.2 MHz, respectively. Our approach enabled discrimination of resistant cells in a group by setting up a threshold provided by the conductivity of running buffer. Subsequent selection of drug resistant cells can be applied to investigate variations in gene expressions and occurrence of mutations related to drug resistance. |
| format |
article |
| author |
Yağmur Demircan Yalçın Taylan Berkin Töral Sertan Sukas Ender Yıldırım Özge Zorlu Ufuk Gündüz Haluk Külah |
| author_facet |
Yağmur Demircan Yalçın Taylan Berkin Töral Sertan Sukas Ender Yıldırım Özge Zorlu Ufuk Gündüz Haluk Külah |
| author_sort |
Yağmur Demircan Yalçın |
| title |
A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| title_short |
A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| title_full |
A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| title_fullStr |
A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| title_full_unstemmed |
A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| title_sort |
microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/4330e938ceb241da919d8e16d7e7f523 |
| work_keys_str_mv |
AT yagmurdemircanyalcın amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT taylanberkintoral amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT sertansukas amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT enderyıldırım amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT ozgezorlu amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT ufukgunduz amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT halukkulah amicrofluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT yagmurdemircanyalcın microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT taylanberkintoral microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT sertansukas microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT enderyıldırım microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT ozgezorlu microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT ufukgunduz microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting AT halukkulah microfluidicdeviceenablingdrugresistanceanalysisofleukemiacellsviacoupleddielectrophoreticdetectionandimpedimetriccounting |
| _version_ |
1718379582177935360 |