Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020

Abstract We monitored the circulating strains and genetic variation among seasonal influenza A and B viruses in Thailand between July 2017 and March 2020. The hemagglutinin gene was amplified and sequenced. We identified amino acid (AA) changes and computed antigenic relatedness using the P epitope...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nungruthai Suntronwong, Sirapa Klinfueng, Sumeth Korkong, Preeyaporn Vichaiwattana, Thanunrat Thongmee, Sompong Vongpunsawad, Yong Poovorawan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4337496ca0914404a0029441d1c1037e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4337496ca0914404a0029441d1c1037e
record_format	dspace
spelling	oai:doaj.org-article:4337496ca0914404a0029441d1c1037e2021-12-02T15:23:10ZCharacterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–202010.1038/s41598-020-80895-w2045-2322https://doaj.org/article/4337496ca0914404a0029441d1c1037e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80895-whttps://doaj.org/toc/2045-2322Abstract We monitored the circulating strains and genetic variation among seasonal influenza A and B viruses in Thailand between July 2017 and March 2020. The hemagglutinin gene was amplified and sequenced. We identified amino acid (AA) changes and computed antigenic relatedness using the P epitope model. Phylogenetic analyses revealed multiple clades/subclades of influenza A(H1N1)pdm09 and A(H3N2) were circulating simultaneously and evolved away from their vaccine strain, but not the influenza B virus. The predominant circulating strains of A(H1N1)pdm09 belonged to 6B.1A1 (2017–2018) and 6B.1A5 (2019–2020) with additional AA substitutions. Clade 3C.2a1b and 3C.2a2 viruses co-circulated in A(H3N2) and clade 3C.3a virus was found in 2020. The B/Victoria-like lineage predominated since 2019 with an additional three AA deletions. Antigenic drift was dominantly facilitated at epitopes Sa and Sb of A(H1N1)pdm09, epitopes A, B, D and E of A(H3N2), and the 120 loop and 190 helix of influenza B virus. Moderate computed antigenic relatedness was observed in A(H1N1)pdm09. The computed antigenic relatedness of A(H3N2) indicated a significant decline in 2019 (9.17%) and 2020 (− 18.94%) whereas the circulating influenza B virus was antigenically similar (94.81%) with its vaccine strain. Our findings offer insights into the genetic divergence from vaccine strains, which could aid vaccine updating.Nungruthai SuntronwongSirapa KlinfuengSumeth KorkongPreeyaporn VichaiwattanaThanunrat ThongmeeSompong VongpunsawadYong PoovorawanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nungruthai Suntronwong Sirapa Klinfueng Sumeth Korkong Preeyaporn Vichaiwattana Thanunrat Thongmee Sompong Vongpunsawad Yong Poovorawan Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020
description	Abstract We monitored the circulating strains and genetic variation among seasonal influenza A and B viruses in Thailand between July 2017 and March 2020. The hemagglutinin gene was amplified and sequenced. We identified amino acid (AA) changes and computed antigenic relatedness using the P epitope model. Phylogenetic analyses revealed multiple clades/subclades of influenza A(H1N1)pdm09 and A(H3N2) were circulating simultaneously and evolved away from their vaccine strain, but not the influenza B virus. The predominant circulating strains of A(H1N1)pdm09 belonged to 6B.1A1 (2017–2018) and 6B.1A5 (2019–2020) with additional AA substitutions. Clade 3C.2a1b and 3C.2a2 viruses co-circulated in A(H3N2) and clade 3C.3a virus was found in 2020. The B/Victoria-like lineage predominated since 2019 with an additional three AA deletions. Antigenic drift was dominantly facilitated at epitopes Sa and Sb of A(H1N1)pdm09, epitopes A, B, D and E of A(H3N2), and the 120 loop and 190 helix of influenza B virus. Moderate computed antigenic relatedness was observed in A(H1N1)pdm09. The computed antigenic relatedness of A(H3N2) indicated a significant decline in 2019 (9.17%) and 2020 (− 18.94%) whereas the circulating influenza B virus was antigenically similar (94.81%) with its vaccine strain. Our findings offer insights into the genetic divergence from vaccine strains, which could aid vaccine updating.
format	article
author	Nungruthai Suntronwong Sirapa Klinfueng Sumeth Korkong Preeyaporn Vichaiwattana Thanunrat Thongmee Sompong Vongpunsawad Yong Poovorawan
author_facet	Nungruthai Suntronwong Sirapa Klinfueng Sumeth Korkong Preeyaporn Vichaiwattana Thanunrat Thongmee Sompong Vongpunsawad Yong Poovorawan
author_sort	Nungruthai Suntronwong
title	Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020
title_short	Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020
title_full	Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020
title_fullStr	Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020
title_full_unstemmed	Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020
title_sort	characterizing genetic and antigenic divergence from vaccine strain of influenza a and b viruses circulating in thailand, 2017–2020
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4337496ca0914404a0029441d1c1037e
work_keys_str_mv	AT nungruthaisuntronwong characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020 AT sirapaklinfueng characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020 AT sumethkorkong characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020 AT preeyapornvichaiwattana characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020 AT thanunratthongmee characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020 AT sompongvongpunsawad characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020 AT yongpoovorawan characterizinggeneticandantigenicdivergencefromvaccinestrainofinfluenzaaandbvirusescirculatinginthailand20172020
_version_	1718387320988631040

Characterizing genetic and antigenic divergence from vaccine strain of influenza A and B viruses circulating in Thailand, 2017–2020

Ejemplares similares