Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development

Bixafen, a pyrazole-carboxamide fungicide, is a potent toxicant that may elicit multiple adverse effects in non-target organisms. However, knowledge of the mechanisms involved in developmental defects caused by bixafen in aquatic organisms remains limited. In this study, the effects of bixafen on re...

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Autores principales:	Peng Xiao, Wenhua Li, Jinfang Lu, Yang Liu, Qiulan Luo, He Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	SDHIs Zebrafish Retinal toxicity Larvae Apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/4338908dffdd4da4a86dc36061546914
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spelling	oai:doaj.org-article:4338908dffdd4da4a86dc360615469142021-11-22T04:16:44ZEffects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development0147-651310.1016/j.ecoenv.2021.113007https://doaj.org/article/4338908dffdd4da4a86dc360615469142021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011192https://doaj.org/toc/0147-6513Bixafen, a pyrazole-carboxamide fungicide, is a potent toxicant that may elicit multiple adverse effects in non-target organisms. However, knowledge of the mechanisms involved in developmental defects caused by bixafen in aquatic organisms remains limited. In this study, the effects of bixafen on retinal development were evaluated in embryo-larval zebrafish. We exposed zebrafish embryos to 0, 0.1, and 0.3 μM bixafen. Exposure of zebrafish embryos to bixafen caused severe retinal defects, including extreme microphthalmia and a significantly increased cell density of the ganglion cell layer (GCL). Compared with the controls, the expression levels of rod and cone photoreceptor marker genes (rho, opn1sw2, opn1mw1, opn1lw1, and opn1sw1) in the outer nuclear layer (ONL) were significantly downregulated after bixafen exposure. Furthermore, bixafen caused significantly increased expression levels in the GCL marker ath5 and decreased expression levels in the inner nuclear layer (INL) markers prox1a, vsx1, and sox2. Accordingly, we observed a significantly increased rate of cell apoptosis in the retina after bixafen exposure. Taken together, our data demonstrate that bixafen exhibits retinal developmental toxicity to zebrafish embryos/larvae, and thus, it may pose a significant environmental threat to aquatic organisms.Peng XiaoWenhua LiJinfang LuYang LiuQiulan LuoHe ZhangElsevierarticleSDHIsZebrafishRetinal toxicityLarvaeApoptosisEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 113007- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SDHIs Zebrafish Retinal toxicity Larvae Apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	SDHIs Zebrafish Retinal toxicity Larvae Apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350 Peng Xiao Wenhua Li Jinfang Lu Yang Liu Qiulan Luo He Zhang Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development
description	Bixafen, a pyrazole-carboxamide fungicide, is a potent toxicant that may elicit multiple adverse effects in non-target organisms. However, knowledge of the mechanisms involved in developmental defects caused by bixafen in aquatic organisms remains limited. In this study, the effects of bixafen on retinal development were evaluated in embryo-larval zebrafish. We exposed zebrafish embryos to 0, 0.1, and 0.3 μM bixafen. Exposure of zebrafish embryos to bixafen caused severe retinal defects, including extreme microphthalmia and a significantly increased cell density of the ganglion cell layer (GCL). Compared with the controls, the expression levels of rod and cone photoreceptor marker genes (rho, opn1sw2, opn1mw1, opn1lw1, and opn1sw1) in the outer nuclear layer (ONL) were significantly downregulated after bixafen exposure. Furthermore, bixafen caused significantly increased expression levels in the GCL marker ath5 and decreased expression levels in the inner nuclear layer (INL) markers prox1a, vsx1, and sox2. Accordingly, we observed a significantly increased rate of cell apoptosis in the retina after bixafen exposure. Taken together, our data demonstrate that bixafen exhibits retinal developmental toxicity to zebrafish embryos/larvae, and thus, it may pose a significant environmental threat to aquatic organisms.
format	article
author	Peng Xiao Wenhua Li Jinfang Lu Yang Liu Qiulan Luo He Zhang
author_facet	Peng Xiao Wenhua Li Jinfang Lu Yang Liu Qiulan Luo He Zhang
author_sort	Peng Xiao
title	Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development
title_short	Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development
title_full	Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development
title_fullStr	Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development
title_full_unstemmed	Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development
title_sort	effects of embryonic exposure to bixafen on zebrafish (danio rerio) retinal development
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4338908dffdd4da4a86dc36061546914
work_keys_str_mv	AT pengxiao effectsofembryonicexposuretobixafenonzebrafishdaniorerioretinaldevelopment AT wenhuali effectsofembryonicexposuretobixafenonzebrafishdaniorerioretinaldevelopment AT jinfanglu effectsofembryonicexposuretobixafenonzebrafishdaniorerioretinaldevelopment AT yangliu effectsofembryonicexposuretobixafenonzebrafishdaniorerioretinaldevelopment AT qiulanluo effectsofembryonicexposuretobixafenonzebrafishdaniorerioretinaldevelopment AT hezhang effectsofembryonicexposuretobixafenonzebrafishdaniorerioretinaldevelopment
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Effects of embryonic exposure to bixafen on zebrafish (Danio rerio) retinal development

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