The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair

Abstract Finding a suitable biomaterial for scaffolding in cartilage tissue engineering has proved to be far from trivial. Nonetheless, it is clear that biomimetic approaches based on gelatin (Gel) and hyaluronic acid (HA) have particular promise. Herein, a set of formulations consisting of photo-po...

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Autores principales:	Yili Wang, Levinus Hendrik Koole, Chenyuan Gao, Dejun Yang, Lei Yang, Chunwu Zhang, Huaqiong Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/433a5bbb7ddc4858a4b9cf4afeeab5f6
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spelling	oai:doaj.org-article:433a5bbb7ddc4858a4b9cf4afeeab5f62021-12-02T19:12:35ZThe potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair10.1038/s41536-021-00166-82057-3995https://doaj.org/article/433a5bbb7ddc4858a4b9cf4afeeab5f62021-09-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00166-8https://doaj.org/toc/2057-3995Abstract Finding a suitable biomaterial for scaffolding in cartilage tissue engineering has proved to be far from trivial. Nonetheless, it is clear that biomimetic approaches based on gelatin (Gel) and hyaluronic acid (HA) have particular promise. Herein, a set of formulations consisting of photo-polymerizable Gel; photo-polymerizable HA, and allogenic decellularized cartilage matrix (DCM), is synthesized and characterized. The novelty of this study lies particularly in the choice of DCM, which was harvested from an abnormal porcine with α-1,3-galactose gene knockout. The hybrid hydrogels were prepared and studied extensively, by spectroscopic methods, for their capacity to imbibe water, for their behavior under compression, and to characterize microstructure. Subsequently, the effects of the hydrogels on contacting cells (in vitro) were studied, i.e., cytotoxicity, morphology, and differentiation through monitoring the specific markers ACAN, Sox9, Coll2, and Col2α1, hypertrophy through monitoring the specific markers alkaline phosphatase (ALP) and Col 10A1. In vivo performance of the hydrogels was assessed in a rat knee cartilage defect model. The new data expand our understanding of hydrogels built of Gel and HA, since they reveal that a significant augmenting role can be played by DCM. The data strongly suggest that further experimentation in larger cartilage-defect animal models is worthwhile and has potential utility for tissue engineering and regenerative medicine.Yili WangLevinus Hendrik KooleChenyuan GaoDejun YangLei YangChunwu ZhangHuaqiong LiNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Yili Wang Levinus Hendrik Koole Chenyuan Gao Dejun Yang Lei Yang Chunwu Zhang Huaqiong Li The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
description	Abstract Finding a suitable biomaterial for scaffolding in cartilage tissue engineering has proved to be far from trivial. Nonetheless, it is clear that biomimetic approaches based on gelatin (Gel) and hyaluronic acid (HA) have particular promise. Herein, a set of formulations consisting of photo-polymerizable Gel; photo-polymerizable HA, and allogenic decellularized cartilage matrix (DCM), is synthesized and characterized. The novelty of this study lies particularly in the choice of DCM, which was harvested from an abnormal porcine with α-1,3-galactose gene knockout. The hybrid hydrogels were prepared and studied extensively, by spectroscopic methods, for their capacity to imbibe water, for their behavior under compression, and to characterize microstructure. Subsequently, the effects of the hydrogels on contacting cells (in vitro) were studied, i.e., cytotoxicity, morphology, and differentiation through monitoring the specific markers ACAN, Sox9, Coll2, and Col2α1, hypertrophy through monitoring the specific markers alkaline phosphatase (ALP) and Col 10A1. In vivo performance of the hydrogels was assessed in a rat knee cartilage defect model. The new data expand our understanding of hydrogels built of Gel and HA, since they reveal that a significant augmenting role can be played by DCM. The data strongly suggest that further experimentation in larger cartilage-defect animal models is worthwhile and has potential utility for tissue engineering and regenerative medicine.
format	article
author	Yili Wang Levinus Hendrik Koole Chenyuan Gao Dejun Yang Lei Yang Chunwu Zhang Huaqiong Li
author_facet	Yili Wang Levinus Hendrik Koole Chenyuan Gao Dejun Yang Lei Yang Chunwu Zhang Huaqiong Li
author_sort	Yili Wang
title	The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
title_short	The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
title_full	The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
title_fullStr	The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
title_full_unstemmed	The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
title_sort	potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/433a5bbb7ddc4858a4b9cf4afeeab5f6
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The potential utility of hybrid photo-crosslinked hydrogels with non-immunogenic component for cartilage repair

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