Alterations in lipid profile upon uterine fibroids and its recurrence

Abstract Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probab...

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Autores principales:	Narine M. Tonoyan, Vitaliy V. Chagovets, Natalia L. Starodubtseva, Alisa O. Tokareva, Konstantin Chingin, Irena F. Kozachenko, Leyla V. Adamyan, Vladimir E. Frankevich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4348f67053cf4420ac39666afba0a85c
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spelling	oai:doaj.org-article:4348f67053cf4420ac39666afba0a85c2021-12-02T15:57:11ZAlterations in lipid profile upon uterine fibroids and its recurrence10.1038/s41598-021-89859-02045-2322https://doaj.org/article/4348f67053cf4420ac39666afba0a85c2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89859-0https://doaj.org/toc/2045-2322Abstract Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction.Narine M. TonoyanVitaliy V. ChagovetsNatalia L. StarodubtsevaAlisa O. TokarevaKonstantin ChinginIrena F. KozachenkoLeyla V. AdamyanVladimir E. FrankevichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Narine M. Tonoyan Vitaliy V. Chagovets Natalia L. Starodubtseva Alisa O. Tokareva Konstantin Chingin Irena F. Kozachenko Leyla V. Adamyan Vladimir E. Frankevich Alterations in lipid profile upon uterine fibroids and its recurrence
description	Abstract Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction.
format	article
author	Narine M. Tonoyan Vitaliy V. Chagovets Natalia L. Starodubtseva Alisa O. Tokareva Konstantin Chingin Irena F. Kozachenko Leyla V. Adamyan Vladimir E. Frankevich
author_facet	Narine M. Tonoyan Vitaliy V. Chagovets Natalia L. Starodubtseva Alisa O. Tokareva Konstantin Chingin Irena F. Kozachenko Leyla V. Adamyan Vladimir E. Frankevich
author_sort	Narine M. Tonoyan
title	Alterations in lipid profile upon uterine fibroids and its recurrence
title_short	Alterations in lipid profile upon uterine fibroids and its recurrence
title_full	Alterations in lipid profile upon uterine fibroids and its recurrence
title_fullStr	Alterations in lipid profile upon uterine fibroids and its recurrence
title_full_unstemmed	Alterations in lipid profile upon uterine fibroids and its recurrence
title_sort	alterations in lipid profile upon uterine fibroids and its recurrence
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4348f67053cf4420ac39666afba0a85c
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Alterations in lipid profile upon uterine fibroids and its recurrence

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