Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells

Summary: A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in micr...

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Autores principales:	Nam-Kyung Yu, Daniel B. McClatchy, Jolene K. Diedrich, Sarah Romero, Jun-Hyeok Choi, Salvador Martínez-Bartolomé, Claire M. Delahunty, Alysson R. Muotri, John R. Yates, III
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Gene network Molecular neuroscience Omics Stem cells research Science Q
Acceso en línea:	https://doaj.org/article/4350b3b7587746e6bd8c1c81b0bf89e6
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spelling	oai:doaj.org-article:4350b3b7587746e6bd8c1c81b0bf89e62021-11-20T05:09:55ZInteractome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells2589-004210.1016/j.isci.2021.103321https://doaj.org/article/4350b3b7587746e6bd8c1c81b0bf89e62021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012906https://doaj.org/toc/2589-0042Summary: A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis, diverse molecular roles have been recognized. In the nervous system, LIN28A is known to play critical roles in proliferation and differentiation of neural progenitor cells (NPCs). We profiled the endogenous LIN28A-interacting proteins in NPCs differentiated from human induced pluripotent stem (iPS) cells using immunoprecipitation and liquid chromatography-tandem mass spectrometry. We identified over 500 LIN28A-interacting proteins, including 156 RNA-independent interactors. Functions of these proteins span a wide range of gene regulatory processes. Prompted by the interactome data, we revealed that LIN28A may impact the subcellular distribution of its interactors and stress granule formation upon oxidative stress. Overall, our analysis opens multiple avenues for elaborating molecular roles and characteristics of LIN28A.Nam-Kyung YuDaniel B. McClatchyJolene K. DiedrichSarah RomeroJun-Hyeok ChoiSalvador Martínez-BartoloméClaire M. DelahuntyAlysson R. MuotriJohn R. Yates, IIIElsevierarticleGene networkMolecular neuroscienceOmicsStem cells researchScienceQENiScience, Vol 24, Iss 11, Pp 103321- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gene network Molecular neuroscience Omics Stem cells research Science Q
spellingShingle	Gene network Molecular neuroscience Omics Stem cells research Science Q Nam-Kyung Yu Daniel B. McClatchy Jolene K. Diedrich Sarah Romero Jun-Hyeok Choi Salvador Martínez-Bartolomé Claire M. Delahunty Alysson R. Muotri John R. Yates, III Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
description	Summary: A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis, diverse molecular roles have been recognized. In the nervous system, LIN28A is known to play critical roles in proliferation and differentiation of neural progenitor cells (NPCs). We profiled the endogenous LIN28A-interacting proteins in NPCs differentiated from human induced pluripotent stem (iPS) cells using immunoprecipitation and liquid chromatography-tandem mass spectrometry. We identified over 500 LIN28A-interacting proteins, including 156 RNA-independent interactors. Functions of these proteins span a wide range of gene regulatory processes. Prompted by the interactome data, we revealed that LIN28A may impact the subcellular distribution of its interactors and stress granule formation upon oxidative stress. Overall, our analysis opens multiple avenues for elaborating molecular roles and characteristics of LIN28A.
format	article
author	Nam-Kyung Yu Daniel B. McClatchy Jolene K. Diedrich Sarah Romero Jun-Hyeok Choi Salvador Martínez-Bartolomé Claire M. Delahunty Alysson R. Muotri John R. Yates, III
author_facet	Nam-Kyung Yu Daniel B. McClatchy Jolene K. Diedrich Sarah Romero Jun-Hyeok Choi Salvador Martínez-Bartolomé Claire M. Delahunty Alysson R. Muotri John R. Yates, III
author_sort	Nam-Kyung Yu
title	Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
title_short	Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
title_full	Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
title_fullStr	Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
title_full_unstemmed	Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells
title_sort	interactome analysis illustrates diverse gene regulatory processes associated with lin28a in human ips cell-derived neural progenitor cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4350b3b7587746e6bd8c1c81b0bf89e6
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Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells

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