Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT

Cancer, which is often described as an uncontrollable rapid proliferation of cells, is currently the leading cause of death in the world together with cardiac disease. Therefore, the main purpose of the current research work was to study the anticancer effects of a first-time-synthesized phthalocyan...

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Autores principales:	Mevlude CANLİCA, İdil ÇETİN
Formato:	article
Lenguaje:	EN
Publicado:	Turkish Chemical Society 2021
Materias:	metal-free phthalocyanine singlet oxygen in vitro breast cancer cell kinetic Chemistry QD1-999
Acceso en línea:	https://doi.org/10.18596/jotcsa.832628 https://doaj.org/article/4355daac7c90499c9929415e118d79cb
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spelling	oai:doaj.org-article:4355daac7c90499c9929415e118d79cb2021-11-20T09:32:44Z Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT https://doi.org/10.18596/jotcsa.8326282149-0120https://doaj.org/article/4355daac7c90499c9929415e118d79cb2021-11-01T00:00:00Zhttps://dergipark.org.tr/tr/pub/jotcsa/issue/64621/832628https://doaj.org/toc/2149-0120Cancer, which is often described as an uncontrollable rapid proliferation of cells, is currently the leading cause of death in the world together with cardiac disease. Therefore, the main purpose of the current research work was to study the anticancer effects of a first-time-synthesized phthalocyanine (Pc) as photosensitizer in PDT against cancer and evaluate its effects on human cells in vitro. Quantum yields of singlet oxygen photogeneration were in air using the relative method with standard-ZnPc as reference and DPBF as chemical quencher for singlet oxygen. The concentration of DPBF was prepared almost 3 x 10-5 molar to avoid chain reactions induced by DPBF in the presence of singlet oxygen. Solutions of Pc as sensitizer (absorbance = 2.0 at the irradiation wavelength) containing DPBF were prepared in the dark and irradiated in the Q band region using the setup described. DPBF degradation at 417 nm was monitored with UV-Vis spectrophotometry. For in vitro studies, nine different MFPc-1 concentrations (0.2 µM- 0.4 µM- 0.8 µM- 1.6 µM- 3.2 µM- 6.4 µM- 12.8 µM- 25.6 µM- 51.2 µM) applied to MCF-7 and MDA-MB-231 breast cancer cell lines for 24 hours and MTT assay was carried out. After determination of optimum concentration, mitotic index, and apoptotic index values of cell lines were determined with administration of these concentrations. Singlet oxygen quantum yield (Φ), which is a measure of the efficiency, of MFPc-1 was found 0.50, although MFPc-1 is being metal-free phthalocyanine. For in vitro studies after the application of different concentrations to MCF-7 and MDA-MB-231 for 24 hours, the optimum concentration was determined as 12 µM for both cell lines by the MTT assay. After application of the determined optimum concentration for 24, 48 and 72 hours, there was a significant decrease in the mitotic index values and significant increase in the apoptotic index values of both MCF-7 and MDA-MB-231 breast cancer cell lines.Mevlude CANLİCAİdil ÇETİNTurkish Chemical Societyarticlemetal-free phthalocyaninesinglet oxygenin vitrobreast cancercell kineticChemistryQD1-999ENJournal of the Turkish Chemical Society, Section A: Chemistry, Vol 8, Iss 4, Pp 1025 -1034 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metal-free phthalocyanine singlet oxygen in vitro breast cancer cell kinetic Chemistry QD1-999
spellingShingle	metal-free phthalocyanine singlet oxygen in vitro breast cancer cell kinetic Chemistry QD1-999 Mevlude CANLİCA İdil ÇETİN Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT
description	Cancer, which is often described as an uncontrollable rapid proliferation of cells, is currently the leading cause of death in the world together with cardiac disease. Therefore, the main purpose of the current research work was to study the anticancer effects of a first-time-synthesized phthalocyanine (Pc) as photosensitizer in PDT against cancer and evaluate its effects on human cells in vitro. Quantum yields of singlet oxygen photogeneration were in air using the relative method with standard-ZnPc as reference and DPBF as chemical quencher for singlet oxygen. The concentration of DPBF was prepared almost 3 x 10-5 molar to avoid chain reactions induced by DPBF in the presence of singlet oxygen. Solutions of Pc as sensitizer (absorbance = 2.0 at the irradiation wavelength) containing DPBF were prepared in the dark and irradiated in the Q band region using the setup described. DPBF degradation at 417 nm was monitored with UV-Vis spectrophotometry. For in vitro studies, nine different MFPc-1 concentrations (0.2 µM- 0.4 µM- 0.8 µM- 1.6 µM- 3.2 µM- 6.4 µM- 12.8 µM- 25.6 µM- 51.2 µM) applied to MCF-7 and MDA-MB-231 breast cancer cell lines for 24 hours and MTT assay was carried out. After determination of optimum concentration, mitotic index, and apoptotic index values of cell lines were determined with administration of these concentrations. Singlet oxygen quantum yield (Φ), which is a measure of the efficiency, of MFPc-1 was found 0.50, although MFPc-1 is being metal-free phthalocyanine. For in vitro studies after the application of different concentrations to MCF-7 and MDA-MB-231 for 24 hours, the optimum concentration was determined as 12 µM for both cell lines by the MTT assay. After application of the determined optimum concentration for 24, 48 and 72 hours, there was a significant decrease in the mitotic index values and significant increase in the apoptotic index values of both MCF-7 and MDA-MB-231 breast cancer cell lines.
format	article
author	Mevlude CANLİCA İdil ÇETİN
author_facet	Mevlude CANLİCA İdil ÇETİN
author_sort	Mevlude CANLİCA
title	Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT
title_short	Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT
title_full	Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT
title_fullStr	Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT
title_full_unstemmed	Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT
title_sort	anticancer activities of a metal-free phthalocyanine on mcf-7 and mda-mb-231 cells and singlet oxygen production as a photosensitizer in pdt
publisher	Turkish Chemical Society
publishDate	2021
url	https://doi.org/10.18596/jotcsa.832628 https://doaj.org/article/4355daac7c90499c9929415e118d79cb
work_keys_str_mv	AT mevludecanlica anticanceractivitiesofametalfreephthalocyanineonmcf7andmdamb231cellsandsingletoxygenproductionasaphotosensitizerinpdt AT idilcetin anticanceractivitiesofametalfreephthalocyanineonmcf7andmdamb231cellsandsingletoxygenproductionasaphotosensitizerinpdt
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Anticancer activities of a metal-free phthalocyanine on MCF-7 and MDA-MB-231 cells and singlet oxygen production as a photosensitizer in PDT

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