FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis

Abstract Background Osteoporosis is a systemic disease characterized by impaired bone formation, increased bone resorption, and brittle bone fractures. The osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is considered to be a vital process for bone formation....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Li, Xingbiao Wu, Yaohua Shi, Hong Zhao
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	FGD5-AS1 miR-506-3p BMP7 Osteoporosis BMSC Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/4356b6a9215e402f8e40bdbf28a6177f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4356b6a9215e402f8e40bdbf28a6177f
record_format	dspace
spelling	oai:doaj.org-article:4356b6a9215e402f8e40bdbf28a6177f2021-11-14T12:29:41ZFGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis10.1186/s13018-021-02694-x1749-799Xhttps://doaj.org/article/4356b6a9215e402f8e40bdbf28a6177f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13018-021-02694-xhttps://doaj.org/toc/1749-799XAbstract Background Osteoporosis is a systemic disease characterized by impaired bone formation, increased bone resorption, and brittle bone fractures. The osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is considered to be a vital process for bone formation. Numerous studies have reported that long non-coding RNAs (lncRNAs) are involved in the osteogenic differentiation of hBMSCs. The present study aimed to investigate the effect of FGD5 antisense RNA 1 (FGD5-AS1) on osteogenic differentiation. Methods RT-qPCR was performed to detect the expression of FGD5-AS1, miR-506-3p, and osteogenesis-related genes OCN, OPN, OSX, and RUNX2. Western blotting was carried out to detect the protein levels of osteogenesis-related markers. In addition, the regulatory effect of FGD5-AS1 on osteogenic differentiation was detected through alkaline phosphatase (ALP) activity, Alizarin Red S (ARS) staining, and Cell Counting Kit-8 (CCK-8). Bioinformatics analysis and luciferase reporter assay were used to predict and validate the interaction between FGD5-AS1 and miR-506-3p as well as miR-506-3p and bone morphogenetic protein 7 (BMP7). Results The RT-qPCR analysis revealed that FGD5-AS1 was upregulated in hBMSCs following induction of osteogenic differentiation. In addition, FGD5-AS1 knockdown attenuated hBMSC viability and osteogenic differentiation. Bioinformatics analysis and luciferase reporter assays verified that FGD5-AS1 could directly interact with microRNA (miR)-506-3p. Furthermore, miR-506-3p could directly target the 3′-untranslated region (3′-UTR) of BMP7. Additionally, functional assays demonstrated that miR-506-3p silencing could restore the suppressive effect of FGD5-AS1 knockdown on osteogenic differentiation and viability of hBMSCs, and miR-506-3p could attenuate osteogenic differentiation via targeting BMP7. Conclusions Taken together, the results of the present study suggested that FGD5-AS1 could positively regulate the osteogenic differentiation of hBMSCs via targeting the miR-506-3p/BMP7 axis.Jun LiXingbiao WuYaohua ShiHong ZhaoBMCarticleFGD5-AS1miR-506-3pBMP7OsteoporosisBMSCOrthopedic surgeryRD701-811Diseases of the musculoskeletal systemRC925-935ENJournal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FGD5-AS1 miR-506-3p BMP7 Osteoporosis BMSC Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
spellingShingle	FGD5-AS1 miR-506-3p BMP7 Osteoporosis BMSC Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935 Jun Li Xingbiao Wu Yaohua Shi Hong Zhao FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis
description	Abstract Background Osteoporosis is a systemic disease characterized by impaired bone formation, increased bone resorption, and brittle bone fractures. The osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is considered to be a vital process for bone formation. Numerous studies have reported that long non-coding RNAs (lncRNAs) are involved in the osteogenic differentiation of hBMSCs. The present study aimed to investigate the effect of FGD5 antisense RNA 1 (FGD5-AS1) on osteogenic differentiation. Methods RT-qPCR was performed to detect the expression of FGD5-AS1, miR-506-3p, and osteogenesis-related genes OCN, OPN, OSX, and RUNX2. Western blotting was carried out to detect the protein levels of osteogenesis-related markers. In addition, the regulatory effect of FGD5-AS1 on osteogenic differentiation was detected through alkaline phosphatase (ALP) activity, Alizarin Red S (ARS) staining, and Cell Counting Kit-8 (CCK-8). Bioinformatics analysis and luciferase reporter assay were used to predict and validate the interaction between FGD5-AS1 and miR-506-3p as well as miR-506-3p and bone morphogenetic protein 7 (BMP7). Results The RT-qPCR analysis revealed that FGD5-AS1 was upregulated in hBMSCs following induction of osteogenic differentiation. In addition, FGD5-AS1 knockdown attenuated hBMSC viability and osteogenic differentiation. Bioinformatics analysis and luciferase reporter assays verified that FGD5-AS1 could directly interact with microRNA (miR)-506-3p. Furthermore, miR-506-3p could directly target the 3′-untranslated region (3′-UTR) of BMP7. Additionally, functional assays demonstrated that miR-506-3p silencing could restore the suppressive effect of FGD5-AS1 knockdown on osteogenic differentiation and viability of hBMSCs, and miR-506-3p could attenuate osteogenic differentiation via targeting BMP7. Conclusions Taken together, the results of the present study suggested that FGD5-AS1 could positively regulate the osteogenic differentiation of hBMSCs via targeting the miR-506-3p/BMP7 axis.
format	article
author	Jun Li Xingbiao Wu Yaohua Shi Hong Zhao
author_facet	Jun Li Xingbiao Wu Yaohua Shi Hong Zhao
author_sort	Jun Li
title	FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis
title_short	FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis
title_full	FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis
title_fullStr	FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis
title_full_unstemmed	FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis
title_sort	fgd5-as1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the mir-506-3p/bmp7 axis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/4356b6a9215e402f8e40bdbf28a6177f
work_keys_str_mv	AT junli fgd5as1facilitatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsviatargetingthemir5063pbmp7axis AT xingbiaowu fgd5as1facilitatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsviatargetingthemir5063pbmp7axis AT yaohuashi fgd5as1facilitatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsviatargetingthemir5063pbmp7axis AT hongzhao fgd5as1facilitatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsviatargetingthemir5063pbmp7axis
_version_	1718429162476142592

FGD5-AS1 facilitates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via targeting the miR-506-3p/BMP7 axis

Ejemplares similares