EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA

Abstract. The aim of the study is to specify the levels of STAT6, and phospho-STAT6 under the influence of IL-4 in patients with bronchial asthma (BA). The samples from ten healthy controls and thirty-three BA patients with allergic and non-allergic clinical forms of different severity were under in...

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Autores principales:	V. N. Mineev, L. N. Sorokina, M. A. Nyoma
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	bronchial asthma jak-stat-system il-4 stat6 pstat6 lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/435731f8c9c04e368eeaf960847de5cb
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spelling	oai:doaj.org-article:435731f8c9c04e368eeaf960847de5cb2021-11-18T08:03:40ZEFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA1563-06252313-741X10.15789/1563-0625-2009-2-3-177-184https://doaj.org/article/435731f8c9c04e368eeaf960847de5cb2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/289https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The aim of the study is to specify the levels of STAT6, and phospho-STAT6 under the influence of IL-4 in patients with bronchial asthma (BA). The samples from ten healthy controls and thirty-three BA patients with allergic and non-allergic clinical forms of different severity were under investigation. Peripheral blood lymphocytes were treated with 10 ng/ml of IL-4 (Sigma Aldrich, USA) for 1 h. Then the proteins (STAT6 and phospho-STAT6) expressed in peripheral lymphocytes were analyzed by Western blot of cell lysates. Preparation of the cell lysates and Western blotting were carried out using standard procedures. Antibodies against phospho-STAT6 and STAT6 (10 ng/ml) were used (Cell Signaling, USA). Levels of thespecific proteins were standardized according to β-actin (Cell Signaling, USA). Treatment with IL-4 caused an increase of phospho-STAT6 levels in lymphocytes of all BA patients, as compared with control group. In allergic BA, the phospho-STAT6 levels were significantly higher than in non-allergic clinical forms. Expression of STAT6 in lymphocytes of patients with severe BA was significantly higher, as compared to BA of moderate severity. An IL-4-induced activation of the STAT6 transcription factor was revealed in an in vitro system, being mostly expressed in allergic BA. The level of STAT6 may serve as a BA severity index. This study was supported by a «Scholarship of the Year» grant from the St. Petersburg State Medical I. Pavlov University (2007).V. N. MineevL. N. SorokinaM. A. NyomaSPb RAACIarticlebronchial asthmajak-stat-systemil-4stat6pstat6lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 2-3, Pp 177-184 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	bronchial asthma jak-stat-system il-4 stat6 pstat6 lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	bronchial asthma jak-stat-system il-4 stat6 pstat6 lymphocytes Immunologic diseases. Allergy RC581-607 V. N. Mineev L. N. Sorokina M. A. Nyoma EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
description	Abstract. The aim of the study is to specify the levels of STAT6, and phospho-STAT6 under the influence of IL-4 in patients with bronchial asthma (BA). The samples from ten healthy controls and thirty-three BA patients with allergic and non-allergic clinical forms of different severity were under investigation. Peripheral blood lymphocytes were treated with 10 ng/ml of IL-4 (Sigma Aldrich, USA) for 1 h. Then the proteins (STAT6 and phospho-STAT6) expressed in peripheral lymphocytes were analyzed by Western blot of cell lysates. Preparation of the cell lysates and Western blotting were carried out using standard procedures. Antibodies against phospho-STAT6 and STAT6 (10 ng/ml) were used (Cell Signaling, USA). Levels of thespecific proteins were standardized according to β-actin (Cell Signaling, USA). Treatment with IL-4 caused an increase of phospho-STAT6 levels in lymphocytes of all BA patients, as compared with control group. In allergic BA, the phospho-STAT6 levels were significantly higher than in non-allergic clinical forms. Expression of STAT6 in lymphocytes of patients with severe BA was significantly higher, as compared to BA of moderate severity. An IL-4-induced activation of the STAT6 transcription factor was revealed in an in vitro system, being mostly expressed in allergic BA. The level of STAT6 may serve as a BA severity index. This study was supported by a «Scholarship of the Year» grant from the St. Petersburg State Medical I. Pavlov University (2007).
format	article
author	V. N. Mineev L. N. Sorokina M. A. Nyoma
author_facet	V. N. Mineev L. N. Sorokina M. A. Nyoma
author_sort	V. N. Mineev
title	EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_short	EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_full	EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_fullStr	EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_full_unstemmed	EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
title_sort	effects of il-4 upon the activity of stat6 transcription factor in peripheral blood lymphocytes in bronchial asthma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/435731f8c9c04e368eeaf960847de5cb
work_keys_str_mv	AT vnmineev effectsofil4upontheactivityofstat6transcriptionfactorinperipheralbloodlymphocytesinbronchialasthma AT lnsorokina effectsofil4upontheactivityofstat6transcriptionfactorinperipheralbloodlymphocytesinbronchialasthma AT manyoma effectsofil4upontheactivityofstat6transcriptionfactorinperipheralbloodlymphocytesinbronchialasthma
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EFFECTS OF IL-4 UPON THE ACTIVITY OF STAT6 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA

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