High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation

Abstract Microbeam radiotherapy (MRT) is based on a spatial fractionation of synchrotron X-ray microbeams at the microscale level. Although the tissue-sparing effect (TSE) in response to non-uniform radiation fields was recognized more than one century ago, the TSE of MRT in the testes and its clini...

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Autores principales:	Hisanori Fukunaga, Kiichi Kaminaga, Takuya Sato, Karl T. Butterworth, Ritsuko Watanabe, Noriko Usami, Takehiko Ogawa, Akinari Yokoya, Kevin M. Prise
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4359fc8125a84c17ae1d481fc0cd10d4
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spelling	oai:doaj.org-article:4359fc8125a84c17ae1d481fc0cd10d42021-12-02T15:07:53ZHigh-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation10.1038/s41598-019-48772-32045-2322https://doaj.org/article/4359fc8125a84c17ae1d481fc0cd10d42019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48772-3https://doaj.org/toc/2045-2322Abstract Microbeam radiotherapy (MRT) is based on a spatial fractionation of synchrotron X-ray microbeams at the microscale level. Although the tissue-sparing effect (TSE) in response to non-uniform radiation fields was recognized more than one century ago, the TSE of MRT in the testes and its clinical importance for preventing male fertility remain to be determined. In this study, using the combination of MRT techniques and a unique ex vivo testes organ culture, we show, for the first time, the MRT-mediated TSE for the preservation of spermatogenesis. Furthermore, our high-precision microbeam analysis revealed that the survival and potential migration steps of the non-irradiated germ stem cells in the irradiated testes tissue would be needed for the effective TSE for spermatogenesis. Our findings indicated the distribution of dose irradiated in the testes at the microscale level is of clinical importance for delivering high doses of radiation to the tumor, while still preserving male fertility.Hisanori FukunagaKiichi KaminagaTakuya SatoKarl T. ButterworthRitsuko WatanabeNoriko UsamiTakehiko OgawaAkinari YokoyaKevin M. PriseNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hisanori Fukunaga Kiichi Kaminaga Takuya Sato Karl T. Butterworth Ritsuko Watanabe Noriko Usami Takehiko Ogawa Akinari Yokoya Kevin M. Prise High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
description	Abstract Microbeam radiotherapy (MRT) is based on a spatial fractionation of synchrotron X-ray microbeams at the microscale level. Although the tissue-sparing effect (TSE) in response to non-uniform radiation fields was recognized more than one century ago, the TSE of MRT in the testes and its clinical importance for preventing male fertility remain to be determined. In this study, using the combination of MRT techniques and a unique ex vivo testes organ culture, we show, for the first time, the MRT-mediated TSE for the preservation of spermatogenesis. Furthermore, our high-precision microbeam analysis revealed that the survival and potential migration steps of the non-irradiated germ stem cells in the irradiated testes tissue would be needed for the effective TSE for spermatogenesis. Our findings indicated the distribution of dose irradiated in the testes at the microscale level is of clinical importance for delivering high doses of radiation to the tumor, while still preserving male fertility.
format	article
author	Hisanori Fukunaga Kiichi Kaminaga Takuya Sato Karl T. Butterworth Ritsuko Watanabe Noriko Usami Takehiko Ogawa Akinari Yokoya Kevin M. Prise
author_facet	Hisanori Fukunaga Kiichi Kaminaga Takuya Sato Karl T. Butterworth Ritsuko Watanabe Noriko Usami Takehiko Ogawa Akinari Yokoya Kevin M. Prise
author_sort	Hisanori Fukunaga
title	High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
title_short	High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
title_full	High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
title_fullStr	High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
title_full_unstemmed	High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
title_sort	high-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4359fc8125a84c17ae1d481fc0cd10d4
work_keys_str_mv	AT hisanorifukunaga highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT kiichikaminaga highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT takuyasato highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT karltbutterworth highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT ritsukowatanabe highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT norikousami highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT takehikoogawa highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT akinariyokoya highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation AT kevinmprise highprecisionmicrobeamradiotherapyrevealstesticulartissuesparingeffectsformalefertilitypreservation
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High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation

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