Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)

The European grapevine moth (EGVM) <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensi...

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Autores principales:	Ignacio Vicente-Díez, Rubén Blanco-Pérez, Maryam Chelkha, Miguel Puelles, Alicia Pou, Raquel Campos-Herrera
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell-free supernatant grape <i>Heterorhabditis</i> <i>Photorhabdus</i> <i>Steinernema</i> secondary metabolites Science Q
Acceso en línea:	https://doaj.org/article/435ab6c860b44dba8078356d0a1fa7d2
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spelling	oai:doaj.org-article:435ab6c860b44dba8078356d0a1fa7d22021-11-25T17:59:57ZExploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)10.3390/insects121110332075-4450https://doaj.org/article/435ab6c860b44dba8078356d0a1fa7d22021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/1033https://doaj.org/toc/2075-4450The European grapevine moth (EGVM) <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensive pesticide use. The entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are well-known virulent agents against arthropod pests thanks to symbiotic bacteria in the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> (respectively) that produce natural products with insecticidal potential. Novel technological advances allow field applications of EPNs and those bioactive compounds as powerful bio-tools against aerial insect pests. This study aimed to determine the viability of four EPN species (<i>Steinernema feltiae</i>, <i>S. carpocapsae</i>, <i>S. riojaense</i>, and <i>Heterorhabditis bacteriophora</i>) as biological control agents against EGVM larval instars (L1, L3, and L5) and pupae. Additionally, the bioactive compounds from their four symbiotic bacteria (<i>Xenorhabdus bovienii</i>, <i>X. nematophila</i>, <i>X. kozodoii</i>, and <i>Photorhabdus laumondii</i> subsp. <i>laumondii,</i> respectively) were tested as unfiltered ferment (UF) and cell-free supernatant (CFS) against the EGVM larval instars L1 and L3. All of the EPN species showed the capability of killing EGVM during the larval and pupal stages, particularly <i>S. carpocapsae</i> (mortalities of ~50% for L1 and >75% for L3 and L5 in only two days), followed by efficacy by <i>S. feltiae</i>. Similarly, the bacterial bioactive compounds produced higher larval mortality at three days against L1 (>90%) than L3 (~50%), making the application of UF more virulent than the application of CFS. Our findings indicate that both steinernematid species and their symbiotic bacterial bioactive compounds could be considered for a novel agro-technological approach to control <i>L. botrana</i> in vineyards. Further research into co-formulation with adjuvants is required to expand their viability when implemented for aboveground grapevine application.Ignacio Vicente-DíezRubén Blanco-PérezMaryam ChelkhaMiguel PuellesAlicia PouRaquel Campos-HerreraMDPI AGarticlecell-free supernatantgrape<i>Heterorhabditis</i><i>Photorhabdus</i><i>Steinernema</i>secondary metabolitesScienceQENInsects, Vol 12, Iss 1033, p 1033 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell-free supernatant grape <i>Heterorhabditis</i> <i>Photorhabdus</i> <i>Steinernema</i> secondary metabolites Science Q
spellingShingle	cell-free supernatant grape <i>Heterorhabditis</i> <i>Photorhabdus</i> <i>Steinernema</i> secondary metabolites Science Q Ignacio Vicente-Díez Rubén Blanco-Pérez Maryam Chelkha Miguel Puelles Alicia Pou Raquel Campos-Herrera Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
description	The European grapevine moth (EGVM) <i>Lobesia botrana</i> (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensive pesticide use. The entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are well-known virulent agents against arthropod pests thanks to symbiotic bacteria in the genera <i>Xenorhabdus</i> and <i>Photorhabdus</i> (respectively) that produce natural products with insecticidal potential. Novel technological advances allow field applications of EPNs and those bioactive compounds as powerful bio-tools against aerial insect pests. This study aimed to determine the viability of four EPN species (<i>Steinernema feltiae</i>, <i>S. carpocapsae</i>, <i>S. riojaense</i>, and <i>Heterorhabditis bacteriophora</i>) as biological control agents against EGVM larval instars (L1, L3, and L5) and pupae. Additionally, the bioactive compounds from their four symbiotic bacteria (<i>Xenorhabdus bovienii</i>, <i>X. nematophila</i>, <i>X. kozodoii</i>, and <i>Photorhabdus laumondii</i> subsp. <i>laumondii,</i> respectively) were tested as unfiltered ferment (UF) and cell-free supernatant (CFS) against the EGVM larval instars L1 and L3. All of the EPN species showed the capability of killing EGVM during the larval and pupal stages, particularly <i>S. carpocapsae</i> (mortalities of ~50% for L1 and >75% for L3 and L5 in only two days), followed by efficacy by <i>S. feltiae</i>. Similarly, the bacterial bioactive compounds produced higher larval mortality at three days against L1 (>90%) than L3 (~50%), making the application of UF more virulent than the application of CFS. Our findings indicate that both steinernematid species and their symbiotic bacterial bioactive compounds could be considered for a novel agro-technological approach to control <i>L. botrana</i> in vineyards. Further research into co-formulation with adjuvants is required to expand their viability when implemented for aboveground grapevine application.
format	article
author	Ignacio Vicente-Díez Rubén Blanco-Pérez Maryam Chelkha Miguel Puelles Alicia Pou Raquel Campos-Herrera
author_facet	Ignacio Vicente-Díez Rubén Blanco-Pérez Maryam Chelkha Miguel Puelles Alicia Pou Raquel Campos-Herrera
author_sort	Ignacio Vicente-Díez
title	Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
title_short	Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
title_full	Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
title_fullStr	Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
title_full_unstemmed	Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)
title_sort	exploring the use of entomopathogenic nematodes and the natural products derived from their symbiotic bacteria to control the grapevine moth, <i>lobesia botrana</i> (lepidoptera: tortricidae)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/435ab6c860b44dba8078356d0a1fa7d2
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Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, <i>Lobesia botrana</i> (Lepidoptera: Tortricidae)

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