The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile

Blood donors with beta-thalassemia traits (βThal<sup>+</sup>) have proven to be good “storers”, since their stored RBCs are resistant to lysis and resilient against oxidative/proteotoxic stress. To examine the performance of these RBCs post-storage, stored βThal<sup>+</sup> a...

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Autores principales:	Alkmini T. Anastasiadi, Efthymios C. Paronis, Vasiliki-Zoi Arvaniti, Athanasios D. Velentzas, Anastasia C. Apostolidou, Evangelos G. Balafas, Monika Dzieciatkowska, Nikolaos G. Kostomitsopoulos, Konstantinos Stamoulis, Issidora S. Papassideri, Angelo D’Alessandro, Anastasios G. Kriebardis, Marianna H. Antonelou, Vassilis L. Tzounakas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	red blood cells blood transfusion beta-thalassemia biomarkers donor variation post-transfusion recovery Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/435b05caaebd470b9f720d563728e375
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spelling	oai:doaj.org-article:435b05caaebd470b9f720d563728e3752021-11-25T17:54:59ZThe Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile10.3390/ijms2222122811422-00671661-6596https://doaj.org/article/435b05caaebd470b9f720d563728e3752021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12281https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Blood donors with beta-thalassemia traits (βThal<sup>+</sup>) have proven to be good “storers”, since their stored RBCs are resistant to lysis and resilient against oxidative/proteotoxic stress. To examine the performance of these RBCs post-storage, stored βThal<sup>+</sup> and control RBCs were reconstituted in plasma donated from transfusion-dependent beta-thalassemic patients and healthy controls, and incubated for 24 h at body temperature. Several physiological parameters, including hemolysis, were evaluated. Moreover, labeled fresh/stored RBCs from the two groups were transfused in mice to assess 24 h recovery. All hemolysis metrics were better in the group of heterozygotes and distinguished them against controls in the plasma environment. The reconstituted βThal<sup>+</sup> samples also presented higher proteasome activity and fewer procoagulant extracellular vesicles. Transfusion to mice demonstrated that βThal<sup>+</sup> RBCs present a marginal trend for higher recovery, regardless of the recipient’s immune background and the RBC storage age. According to correlation analysis, several of these advantageous post-storage characteristics are related to storage phenotypes, like the cytoskeleton composition, low cellular fragility, and enhanced membrane proteostasis that characterize stored βThal<sup>+</sup> RBCs. Overall, it seems that the intrinsic physiology of βThal<sup>+</sup> RBCs benefits them in conditions mimicking a recipient environment, and in the circulation of animal models; findings that warrant validation in clinical trials.Alkmini T. AnastasiadiEfthymios C. ParonisVasiliki-Zoi ArvanitiAthanasios D. VelentzasAnastasia C. ApostolidouEvangelos G. BalafasMonika DzieciatkowskaNikolaos G. KostomitsopoulosKonstantinos StamoulisIssidora S. PapassideriAngelo D’AlessandroAnastasios G. KriebardisMarianna H. AntonelouVassilis L. TzounakasMDPI AGarticlered blood cellsblood transfusionbeta-thalassemiabiomarkersdonor variationpost-transfusion recoveryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12281, p 12281 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	red blood cells blood transfusion beta-thalassemia biomarkers donor variation post-transfusion recovery Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	red blood cells blood transfusion beta-thalassemia biomarkers donor variation post-transfusion recovery Biology (General) QH301-705.5 Chemistry QD1-999 Alkmini T. Anastasiadi Efthymios C. Paronis Vasiliki-Zoi Arvaniti Athanasios D. Velentzas Anastasia C. Apostolidou Evangelos G. Balafas Monika Dzieciatkowska Nikolaos G. Kostomitsopoulos Konstantinos Stamoulis Issidora S. Papassideri Angelo D’Alessandro Anastasios G. Kriebardis Marianna H. Antonelou Vassilis L. Tzounakas The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile
description	Blood donors with beta-thalassemia traits (βThal<sup>+</sup>) have proven to be good “storers”, since their stored RBCs are resistant to lysis and resilient against oxidative/proteotoxic stress. To examine the performance of these RBCs post-storage, stored βThal<sup>+</sup> and control RBCs were reconstituted in plasma donated from transfusion-dependent beta-thalassemic patients and healthy controls, and incubated for 24 h at body temperature. Several physiological parameters, including hemolysis, were evaluated. Moreover, labeled fresh/stored RBCs from the two groups were transfused in mice to assess 24 h recovery. All hemolysis metrics were better in the group of heterozygotes and distinguished them against controls in the plasma environment. The reconstituted βThal<sup>+</sup> samples also presented higher proteasome activity and fewer procoagulant extracellular vesicles. Transfusion to mice demonstrated that βThal<sup>+</sup> RBCs present a marginal trend for higher recovery, regardless of the recipient’s immune background and the RBC storage age. According to correlation analysis, several of these advantageous post-storage characteristics are related to storage phenotypes, like the cytoskeleton composition, low cellular fragility, and enhanced membrane proteostasis that characterize stored βThal<sup>+</sup> RBCs. Overall, it seems that the intrinsic physiology of βThal<sup>+</sup> RBCs benefits them in conditions mimicking a recipient environment, and in the circulation of animal models; findings that warrant validation in clinical trials.
format	article
author	Alkmini T. Anastasiadi Efthymios C. Paronis Vasiliki-Zoi Arvaniti Athanasios D. Velentzas Anastasia C. Apostolidou Evangelos G. Balafas Monika Dzieciatkowska Nikolaos G. Kostomitsopoulos Konstantinos Stamoulis Issidora S. Papassideri Angelo D’Alessandro Anastasios G. Kriebardis Marianna H. Antonelou Vassilis L. Tzounakas
author_facet	Alkmini T. Anastasiadi Efthymios C. Paronis Vasiliki-Zoi Arvaniti Athanasios D. Velentzas Anastasia C. Apostolidou Evangelos G. Balafas Monika Dzieciatkowska Nikolaos G. Kostomitsopoulos Konstantinos Stamoulis Issidora S. Papassideri Angelo D’Alessandro Anastasios G. Kriebardis Marianna H. Antonelou Vassilis L. Tzounakas
author_sort	Alkmini T. Anastasiadi
title	The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile
title_short	The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile
title_full	The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile
title_fullStr	The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile
title_full_unstemmed	The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile
title_sort	post-storage performance of rbcs from beta-thalassemia trait donors is related to their storability profile
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/435b05caaebd470b9f720d563728e375
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The Post-Storage Performance of RBCs from Beta-Thalassemia Trait Donors Is Related to Their Storability Profile

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