Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis

The standard serological test such as Rose Bengal Plate Test (RBPT) is routinely used for the diagnosis of brucellosis. This test depends on the agglutination of colored particulate antigen (killed Brucella organisms) by the antibodies present in sera of infected animals. Faulty negative and positi...

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Autores principales:	Lamees A. El-Tantawy, Lubna, F. Farahat, Eman S. Ramadan, Fatma F. Warda
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	brucella cft elisa rbpt stat Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.85550 https://doaj.org/article/43612ab7dde0499481d127f97d987f63
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spelling	oai:doaj.org-article:43612ab7dde0499481d127f97d987f632021-12-02T13:24:01ZEffects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosishttps://dx.doi.org/10.21608/javs.2020.855501687-40722090-3308https://doaj.org/article/43612ab7dde0499481d127f97d987f632020-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_85550.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308The standard serological test such as Rose Bengal Plate Test (RBPT) is routinely used for the diagnosis of brucellosis. This test depends on the agglutination of colored particulate antigen (killed Brucella organisms) by the antibodies present in sera of infected animals. Faulty negative and positive results are commonly experienced in these traditional agglutination tests. We developed three simple, new, additional and cost-effective steps that can help in these problems; Superagglutination test for serodiagnosis of brucellosis differs from conventional RBPT by three simple new and coast effective additional steps which are used to overcome this problem. These steps depend on the staining of sera antibodies by adding dye before the test and addition of diluted biotinylated antiglobulin and Avidin in sequence then mixing the antigen with the stained serum. By testing 150 serum samples, Superagglutination test had higher positive predictive value and specificity than RBPT and standard tube agglutination test (STAT). Also, it had higher negative predictive value and sensitivity than RBPT, STAT, ELISA (Indirect enzyme-linked immunosorbent assay) and CFT (Complement Fixation test). Lamees A. El-TantawyLubna, F. FarahatEman S. RamadanFatma F. WardaEgyptian Society for Animal ManagementarticlebrucellacftelisarbptstatZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 2, Pp 6-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	brucella cft elisa rbpt stat Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	brucella cft elisa rbpt stat Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Lamees A. El-Tantawy Lubna, F. Farahat Eman S. Ramadan Fatma F. Warda Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
description	The standard serological test such as Rose Bengal Plate Test (RBPT) is routinely used for the diagnosis of brucellosis. This test depends on the agglutination of colored particulate antigen (killed Brucella organisms) by the antibodies present in sera of infected animals. Faulty negative and positive results are commonly experienced in these traditional agglutination tests. We developed three simple, new, additional and cost-effective steps that can help in these problems; Superagglutination test for serodiagnosis of brucellosis differs from conventional RBPT by three simple new and coast effective additional steps which are used to overcome this problem. These steps depend on the staining of sera antibodies by adding dye before the test and addition of diluted biotinylated antiglobulin and Avidin in sequence then mixing the antigen with the stained serum. By testing 150 serum samples, Superagglutination test had higher positive predictive value and specificity than RBPT and standard tube agglutination test (STAT). Also, it had higher negative predictive value and sensitivity than RBPT, STAT, ELISA (Indirect enzyme-linked immunosorbent assay) and CFT (Complement Fixation test).
format	article
author	Lamees A. El-Tantawy Lubna, F. Farahat Eman S. Ramadan Fatma F. Warda
author_facet	Lamees A. El-Tantawy Lubna, F. Farahat Eman S. Ramadan Fatma F. Warda
author_sort	Lamees A. El-Tantawy
title	Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
title_short	Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
title_full	Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
title_fullStr	Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
title_full_unstemmed	Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
title_sort	effects of chemically pretreated bovine serum samples on sensitivity of rose bengal test for serodiagnosis of bovine brucellosis
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.85550 https://doaj.org/article/43612ab7dde0499481d127f97d987f63
work_keys_str_mv	AT lameesaeltantawy effectsofchemicallypretreatedbovineserumsamplesonsensitivityofrosebengaltestforserodiagnosisofbovinebrucellosis AT lubnaffarahat effectsofchemicallypretreatedbovineserumsamplesonsensitivityofrosebengaltestforserodiagnosisofbovinebrucellosis AT emansramadan effectsofchemicallypretreatedbovineserumsamplesonsensitivityofrosebengaltestforserodiagnosisofbovinebrucellosis AT fatmafwarda effectsofchemicallypretreatedbovineserumsamplesonsensitivityofrosebengaltestforserodiagnosisofbovinebrucellosis
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Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis

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