Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers

Abstract. Uslan, Pharmawat M. 2020. Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers. Biodiversitas 21: 3407-3414. This study aims to determine the genetic diversity of Sterculia quadrifida R.Br. in Kupang based on RAPD markers....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Uslan Uslan, MADE PHARMAWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	conservation, faloak, herbal, kupang, rapd, sterculia quadrifida Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/43670f6d89304dc2ad9c6d20966606c2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:43670f6d89304dc2ad9c6d20966606c2
record_format	dspace
spelling	oai:doaj.org-article:43670f6d89304dc2ad9c6d20966606c22021-11-22T00:40:51ZGenetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers1412-033X2085-472210.13057/biodiv/d210766https://doaj.org/article/43670f6d89304dc2ad9c6d20966606c22020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6242https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Uslan, Pharmawat M. 2020. Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers. Biodiversitas 21: 3407-3414. This study aims to determine the genetic diversity of Sterculia quadrifida R.Br. in Kupang based on RAPD markers. Samples of S. quadrifida were collected from the yard and community forest in Kupang City (Sub-districts of Oebobo, Kelapa Lima, Maulafa, and Alak) and mixed forest in the Kupang District (Sub-districts of Kupang Barat, Nekamese, Taebenu, and Fatuleu). DNA was isolated by the CTAB method and amplified by six RAPD primers (OPD-11, OPF-11, UBC-106, UBC-127, UBC-250, and OPB-04). The data were analyzed in the MVSP software using UPGMA method and Nei & Li similarity coefficient. Total of 131 DNA bands ranging from 250-1400 bp was obtained. Populations of S. quadrifida in Kupang were divided into two main clusters and 12 sub-clusters with. The highest genetic diversity was found in Kelapa Lima of 0.1050, while the lowest genetic diversity was found in S. quadrifida population in Fatuleu of 0.0305. The population of S. quadrifida in Kupang has high genetic diversity and also clustered based on their geographical distributionUslan UslanMADE PHARMAWATIMBI & UNS Soloarticleconservation, faloak, herbal, kupang, rapd, sterculia quadrifidaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	conservation, faloak, herbal, kupang, rapd, sterculia quadrifida Biology (General) QH301-705.5
spellingShingle	conservation, faloak, herbal, kupang, rapd, sterculia quadrifida Biology (General) QH301-705.5 Uslan Uslan MADE PHARMAWATI Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers
description	Abstract. Uslan, Pharmawat M. 2020. Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers. Biodiversitas 21: 3407-3414. This study aims to determine the genetic diversity of Sterculia quadrifida R.Br. in Kupang based on RAPD markers. Samples of S. quadrifida were collected from the yard and community forest in Kupang City (Sub-districts of Oebobo, Kelapa Lima, Maulafa, and Alak) and mixed forest in the Kupang District (Sub-districts of Kupang Barat, Nekamese, Taebenu, and Fatuleu). DNA was isolated by the CTAB method and amplified by six RAPD primers (OPD-11, OPF-11, UBC-106, UBC-127, UBC-250, and OPB-04). The data were analyzed in the MVSP software using UPGMA method and Nei & Li similarity coefficient. Total of 131 DNA bands ranging from 250-1400 bp was obtained. Populations of S. quadrifida in Kupang were divided into two main clusters and 12 sub-clusters with. The highest genetic diversity was found in Kelapa Lima of 0.1050, while the lowest genetic diversity was found in S. quadrifida population in Fatuleu of 0.0305. The population of S. quadrifida in Kupang has high genetic diversity and also clustered based on their geographical distribution
format	article
author	Uslan Uslan MADE PHARMAWATI
author_facet	Uslan Uslan MADE PHARMAWATI
author_sort	Uslan Uslan
title	Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers
title_short	Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers
title_full	Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers
title_fullStr	Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers
title_full_unstemmed	Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers
title_sort	genetic diversity of sterculia quadrifida in kupang, indonesia based on rapd (random amplified polymorphic dna) markers
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/43670f6d89304dc2ad9c6d20966606c2
work_keys_str_mv	AT uslanuslan geneticdiversityofsterculiaquadrifidainkupangindonesiabasedonrapdrandomamplifiedpolymorphicdnamarkers AT madepharmawati geneticdiversityofsterculiaquadrifidainkupangindonesiabasedonrapdrandomamplifiedpolymorphicdnamarkers
_version_	1718418517606268928

Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers

Ejemplares similares