Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant

The present study aimed to prepare a combined inactivated pneumo-4 vaccine containing bovine viral diarrhoea, infectious bovine rhinotracheitis (IBR), parainfluenza-3(PI-3) and bovine respiratory syncytial virus (BRSV) adjuvanted with carbomer and compare the animal protection scope with its produ...

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Autores principales:	Maha Raafat Abed EL-Fadil, Rasha, I. EL-Hawary, Walaa S. Shabana, Ekbal M. Farok, Naglaa I. Ali
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2019
Materias:	adjuvant aluminium hydroxide gel brsv carbomer pneumo-4 vaccine Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2019.62653 https://doaj.org/article/4368b676eab84520853b620e48c4fb96
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spelling	oai:doaj.org-article:4368b676eab84520853b620e48c4fb962021-12-02T11:43:44ZEnhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvanthttps://dx.doi.org/10.21608/javs.2019.626531687-40722090-3308https://doaj.org/article/4368b676eab84520853b620e48c4fb962019-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62653.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308The present study aimed to prepare a combined inactivated pneumo-4 vaccine containing bovine viral diarrhoea, infectious bovine rhinotracheitis (IBR), parainfluenza-3(PI-3) and bovine respiratory syncytial virus (BRSV) adjuvanted with carbomer and compare the animal protection scope with its product mate Pneumo-4 adjuvanted alhydragel . Carbomer adjuvant stimulated higher levels and longer lasting antibody to pneumo-4 vaccine than vaccines of equivalent antigenic content containing aluminium hydroxide gel adjuvant. Quality control results proved that the pneumo-4 vaccine adjuvanted either by aluminium hydroxide gel adjuvant or carbomer (different concentration 0.2,0.5 and0.8 %) was pure and completely safe to be used in calves without any abnomalities.Potency test was performed on five groups of five calves for each group,Where the first group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.2(formula 2), second group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.5 (formula 3), third group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.8(formula 4),,The fourth group vaccinated with pneumo-4 vaccine with aluminum hydroxide gel(formula 1), and the fifth last group was left as non vaccinated control group.compared humoral immune response using serum neutralization and ELISA expressed by neutralization index of different vaccine formulation was revealed that The prepared pneumo-4 vaccine adjuvant with carbomer 0.5 proved to be highly potent formmula along 6 month after second booster dose applying. Also pneumo-4 vaccine adjuvant with carbomer was showed cellular immunity reaction higer than pneumo-4 vaccine with aluminum hydroxide gel using Estimation of nitric oxide activity test.Maha Raafat Abed EL-FadilRasha, I. EL-HawaryWalaa S. Shabana Ekbal M. Farok Naglaa I. AliEgyptian Society for Animal Managementarticleadjuvantaluminium hydroxide gelbrsvcarbomerpneumo-4 vaccineZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 4, Iss 2, Pp 35-40 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	adjuvant aluminium hydroxide gel brsv carbomer pneumo-4 vaccine Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	adjuvant aluminium hydroxide gel brsv carbomer pneumo-4 vaccine Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Maha Raafat Abed EL-Fadil Rasha, I. EL-Hawary Walaa S. Shabana Ekbal M. Farok Naglaa I. Ali Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant
description	The present study aimed to prepare a combined inactivated pneumo-4 vaccine containing bovine viral diarrhoea, infectious bovine rhinotracheitis (IBR), parainfluenza-3(PI-3) and bovine respiratory syncytial virus (BRSV) adjuvanted with carbomer and compare the animal protection scope with its product mate Pneumo-4 adjuvanted alhydragel . Carbomer adjuvant stimulated higher levels and longer lasting antibody to pneumo-4 vaccine than vaccines of equivalent antigenic content containing aluminium hydroxide gel adjuvant. Quality control results proved that the pneumo-4 vaccine adjuvanted either by aluminium hydroxide gel adjuvant or carbomer (different concentration 0.2,0.5 and0.8 %) was pure and completely safe to be used in calves without any abnomalities.Potency test was performed on five groups of five calves for each group,Where the first group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.2(formula 2), second group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.5 (formula 3), third group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.8(formula 4),,The fourth group vaccinated with pneumo-4 vaccine with aluminum hydroxide gel(formula 1), and the fifth last group was left as non vaccinated control group.compared humoral immune response using serum neutralization and ELISA expressed by neutralization index of different vaccine formulation was revealed that The prepared pneumo-4 vaccine adjuvant with carbomer 0.5 proved to be highly potent formmula along 6 month after second booster dose applying. Also pneumo-4 vaccine adjuvant with carbomer was showed cellular immunity reaction higer than pneumo-4 vaccine with aluminum hydroxide gel using Estimation of nitric oxide activity test.
format	article
author	Maha Raafat Abed EL-Fadil Rasha, I. EL-Hawary Walaa S. Shabana Ekbal M. Farok Naglaa I. Ali
author_facet	Maha Raafat Abed EL-Fadil Rasha, I. EL-Hawary Walaa S. Shabana Ekbal M. Farok Naglaa I. Ali
author_sort	Maha Raafat Abed EL-Fadil
title	Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant
title_short	Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant
title_full	Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant
title_fullStr	Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant
title_full_unstemmed	Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant
title_sort	enhancement of pneumo-4 vaccine efficacy by using of carbomere as an adjuvant
publisher	Egyptian Society for Animal Management
publishDate	2019
url	https://dx.doi.org/10.21608/javs.2019.62653 https://doaj.org/article/4368b676eab84520853b620e48c4fb96
work_keys_str_mv	AT maharaafatabedelfadil enhancementofpneumo4vaccineefficacybyusingofcarbomereasanadjuvant AT rashaielhawary enhancementofpneumo4vaccineefficacybyusingofcarbomereasanadjuvant AT walaasshabana enhancementofpneumo4vaccineefficacybyusingofcarbomereasanadjuvant AT ekbalmfarok enhancementofpneumo4vaccineefficacybyusingofcarbomereasanadjuvant AT naglaaiali enhancementofpneumo4vaccineefficacybyusingofcarbomereasanadjuvant
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Enhancement of Pneumo-4 Vaccine Efficacy by Using of Carbomere as an Adjuvant

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