Disruption of retinal pigment epithelial cell properties under the exposure of cotinine

Abstract Cigarette smoking is a major risk factor for age-related macular degeneration (AMD), in which progressive retinal pigment epithelial (RPE) cell degeneration is a major pathological change. Nicotine is a major biologically active component in cigarette smoke. It is continuously catabolized i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiao-Yu Zhang, Tsz Kin Ng, Mårten Erik Brelén, Kwok Ping Chan, Di Wu, Jasmine Sum Yee Yung, Di Cao, Yumeng Wang, Shaodan Zhang, Sun On Chan, Chi Pui Pang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/436bf9f370d549f8a42d9ff98bad4762
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:436bf9f370d549f8a42d9ff98bad4762
record_format	dspace
spelling	oai:doaj.org-article:436bf9f370d549f8a42d9ff98bad47622021-12-02T11:52:30ZDisruption of retinal pigment epithelial cell properties under the exposure of cotinine10.1038/s41598-017-03283-x2045-2322https://doaj.org/article/436bf9f370d549f8a42d9ff98bad47622017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03283-xhttps://doaj.org/toc/2045-2322Abstract Cigarette smoking is a major risk factor for age-related macular degeneration (AMD), in which progressive retinal pigment epithelial (RPE) cell degeneration is a major pathological change. Nicotine is a major biologically active component in cigarette smoke. It is continuously catabolized into cotinine, which has longer half-life and higher concentration in tissue cells and fluids. Here we hypothesized that continuous exposure of cotinine has more potent effects on human RPE cell properties than nicotine. Human RPE cell line (ARPE-19) was treated continuously with 1–2 µM of nicotine and/or cotinine for 7 days. RPE cells treated with 2 μM cotinine and nicotine-cotinine mixture has lower MTT signals without significant changes in cell apoptosis or integrity. Moreover, RPE cell migration was retarded under cotinine treatments, but not nicotine. Both nicotine and cotinine treatments attenuated the phagocytotic activity of RPE cells. In addition, cotinine and nicotine-cotinine mixture suppressed VEGF and IL-8 expression and upregulated TIMP-2 expression. Expressions of autophagy genes were upregulated by the cotinine treatment, whereas expressions of epithelial-to-mesenchymal transition markers were downregulated. In conclusion, our study, for the first time, demonstrated that cotinine, rather than nicotine, affects the properties of RPE cells in vitro, which could explain the smoking-induced RPE pathology.Xiao-Yu ZhangTsz Kin NgMårten Erik BrelénKwok Ping ChanDi WuJasmine Sum Yee YungDi CaoYumeng WangShaodan ZhangSun On ChanChi Pui PangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao-Yu Zhang Tsz Kin Ng Mårten Erik Brelén Kwok Ping Chan Di Wu Jasmine Sum Yee Yung Di Cao Yumeng Wang Shaodan Zhang Sun On Chan Chi Pui Pang Disruption of retinal pigment epithelial cell properties under the exposure of cotinine
description	Abstract Cigarette smoking is a major risk factor for age-related macular degeneration (AMD), in which progressive retinal pigment epithelial (RPE) cell degeneration is a major pathological change. Nicotine is a major biologically active component in cigarette smoke. It is continuously catabolized into cotinine, which has longer half-life and higher concentration in tissue cells and fluids. Here we hypothesized that continuous exposure of cotinine has more potent effects on human RPE cell properties than nicotine. Human RPE cell line (ARPE-19) was treated continuously with 1–2 µM of nicotine and/or cotinine for 7 days. RPE cells treated with 2 μM cotinine and nicotine-cotinine mixture has lower MTT signals without significant changes in cell apoptosis or integrity. Moreover, RPE cell migration was retarded under cotinine treatments, but not nicotine. Both nicotine and cotinine treatments attenuated the phagocytotic activity of RPE cells. In addition, cotinine and nicotine-cotinine mixture suppressed VEGF and IL-8 expression and upregulated TIMP-2 expression. Expressions of autophagy genes were upregulated by the cotinine treatment, whereas expressions of epithelial-to-mesenchymal transition markers were downregulated. In conclusion, our study, for the first time, demonstrated that cotinine, rather than nicotine, affects the properties of RPE cells in vitro, which could explain the smoking-induced RPE pathology.
format	article
author	Xiao-Yu Zhang Tsz Kin Ng Mårten Erik Brelén Kwok Ping Chan Di Wu Jasmine Sum Yee Yung Di Cao Yumeng Wang Shaodan Zhang Sun On Chan Chi Pui Pang
author_facet	Xiao-Yu Zhang Tsz Kin Ng Mårten Erik Brelén Kwok Ping Chan Di Wu Jasmine Sum Yee Yung Di Cao Yumeng Wang Shaodan Zhang Sun On Chan Chi Pui Pang
author_sort	Xiao-Yu Zhang
title	Disruption of retinal pigment epithelial cell properties under the exposure of cotinine
title_short	Disruption of retinal pigment epithelial cell properties under the exposure of cotinine
title_full	Disruption of retinal pigment epithelial cell properties under the exposure of cotinine
title_fullStr	Disruption of retinal pigment epithelial cell properties under the exposure of cotinine
title_full_unstemmed	Disruption of retinal pigment epithelial cell properties under the exposure of cotinine
title_sort	disruption of retinal pigment epithelial cell properties under the exposure of cotinine
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/436bf9f370d549f8a42d9ff98bad4762
work_keys_str_mv	AT xiaoyuzhang disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT tszkinng disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT martenerikbrelen disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT kwokpingchan disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT diwu disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT jasminesumyeeyung disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT dicao disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT yumengwang disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT shaodanzhang disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT sunonchan disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine AT chipuipang disruptionofretinalpigmentepithelialcellpropertiesundertheexposureofcotinine
_version_	1718395043479289856

Disruption of retinal pigment epithelial cell properties under the exposure of cotinine

Ejemplares similares