High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut

ABSTRACT Dietary fat intake and shifts in gut bacterial community composition are associated with the development of obesity. To date, characterization of microbiota in lean versus obese subjects has been dominated by studies of gut bacteria. Fungi, recently shown to affect gut inflammation, have re...

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Autores principales:	Timothy Heisel, Emmanuel Montassier, Abigail Johnson, Gabriel Al-Ghalith, Yi-Wei Lin, Li-Na Wei, Dan Knights, Cheryl A. Gale
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	fungal-bacterial interactions fungi high-fat diet microbiome obesity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/436c5d04f5af4c44b3c4d67c471a5bc4
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spelling	oai:doaj.org-article:436c5d04f5af4c44b3c4d67c471a5bc42021-11-15T15:22:05ZHigh-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut10.1128/mSphere.00351-172379-5042https://doaj.org/article/436c5d04f5af4c44b3c4d67c471a5bc42017-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00351-17https://doaj.org/toc/2379-5042ABSTRACT Dietary fat intake and shifts in gut bacterial community composition are associated with the development of obesity. To date, characterization of microbiota in lean versus obese subjects has been dominated by studies of gut bacteria. Fungi, recently shown to affect gut inflammation, have received little study for their role in obesity. We sought to determine the effects of high-fat diet on fungal and bacterial community structures in a mouse model using the internal transcribed spacer region 2 (ITS2) of fungal ribosomal DNA (rDNA) and the 16S rRNA genes of bacteria. Mice fed a high-fat diet had significantly different abundances of 19 bacterial and 6 fungal taxa than did mice fed standard chow, with high-fat diet causing similar magnitudes of change in overall fungal and bacterial microbiome structures. We observed strong and complex diet-specific coabundance relationships between intra- and interkingdom microbial pairs and dramatic reductions in the number of coabundance correlations in mice fed a high-fat diet compared to those fed standard chow. Furthermore, predicted microbiome functional modules related to metabolism were significantly less abundant in high-fat-diet-fed than in standard-chow-fed mice. These results suggest a role for fungi and interkingdom interactions in the association between gut microbiomes and obesity. IMPORTANCE Recent research shows that gut microbes are involved in the development of obesity, a growing health problem in developed countries that is linked to increased risk for cardiovascular disease. However, studies showing links between microbes and metabolism have been limited to the analysis of bacteria and have ignored the potential contribution of fungi in metabolic health. This study provides evidence that ingestion of a high-fat diet is associated with changes to the fungal (and bacterial) microbiome in a mouse model. In addition, we find that interkingdom structural and functional relationships exist between fungi and bacteria within the gut and that these are perturbed by high-fat diet.Timothy HeiselEmmanuel MontassierAbigail JohnsonGabriel Al-GhalithYi-Wei LinLi-Na WeiDan KnightsCheryl A. GaleAmerican Society for Microbiologyarticlefungal-bacterial interactionsfungihigh-fat dietmicrobiomeobesityMicrobiologyQR1-502ENmSphere, Vol 2, Iss 5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	fungal-bacterial interactions fungi high-fat diet microbiome obesity Microbiology QR1-502
spellingShingle	fungal-bacterial interactions fungi high-fat diet microbiome obesity Microbiology QR1-502 Timothy Heisel Emmanuel Montassier Abigail Johnson Gabriel Al-Ghalith Yi-Wei Lin Li-Na Wei Dan Knights Cheryl A. Gale High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut
description	ABSTRACT Dietary fat intake and shifts in gut bacterial community composition are associated with the development of obesity. To date, characterization of microbiota in lean versus obese subjects has been dominated by studies of gut bacteria. Fungi, recently shown to affect gut inflammation, have received little study for their role in obesity. We sought to determine the effects of high-fat diet on fungal and bacterial community structures in a mouse model using the internal transcribed spacer region 2 (ITS2) of fungal ribosomal DNA (rDNA) and the 16S rRNA genes of bacteria. Mice fed a high-fat diet had significantly different abundances of 19 bacterial and 6 fungal taxa than did mice fed standard chow, with high-fat diet causing similar magnitudes of change in overall fungal and bacterial microbiome structures. We observed strong and complex diet-specific coabundance relationships between intra- and interkingdom microbial pairs and dramatic reductions in the number of coabundance correlations in mice fed a high-fat diet compared to those fed standard chow. Furthermore, predicted microbiome functional modules related to metabolism were significantly less abundant in high-fat-diet-fed than in standard-chow-fed mice. These results suggest a role for fungi and interkingdom interactions in the association between gut microbiomes and obesity. IMPORTANCE Recent research shows that gut microbes are involved in the development of obesity, a growing health problem in developed countries that is linked to increased risk for cardiovascular disease. However, studies showing links between microbes and metabolism have been limited to the analysis of bacteria and have ignored the potential contribution of fungi in metabolic health. This study provides evidence that ingestion of a high-fat diet is associated with changes to the fungal (and bacterial) microbiome in a mouse model. In addition, we find that interkingdom structural and functional relationships exist between fungi and bacteria within the gut and that these are perturbed by high-fat diet.
format	article
author	Timothy Heisel Emmanuel Montassier Abigail Johnson Gabriel Al-Ghalith Yi-Wei Lin Li-Na Wei Dan Knights Cheryl A. Gale
author_facet	Timothy Heisel Emmanuel Montassier Abigail Johnson Gabriel Al-Ghalith Yi-Wei Lin Li-Na Wei Dan Knights Cheryl A. Gale
author_sort	Timothy Heisel
title	High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut
title_short	High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut
title_full	High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut
title_fullStr	High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut
title_full_unstemmed	High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut
title_sort	high-fat diet changes fungal microbiomes and interkingdom relationships in the murine gut
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/436c5d04f5af4c44b3c4d67c471a5bc4
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High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut

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