3D models of the hematopoietic stem cell niche under steady-state and active conditions

Abstract Hematopoietic stem cells (HSCs) in the bone marrow are able to differentiate into all types of blood cells and supply the organism each day with billions of fresh cells. They are applied to cure hematological diseases such as leukemia. The clinical need for HSCs is high and there is a deman...

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Autores principales:	Lisa Rödling, Ivo Schwedhelm, Saskia Kraus, Karen Bieback, Jan Hansmann, Cornelia Lee-Thedieck
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4375f52eee9a49e89c5477d4f7a5411c
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spelling	oai:doaj.org-article:4375f52eee9a49e89c5477d4f7a5411c2021-12-02T12:32:32Z3D models of the hematopoietic stem cell niche under steady-state and active conditions10.1038/s41598-017-04808-02045-2322https://doaj.org/article/4375f52eee9a49e89c5477d4f7a5411c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04808-0https://doaj.org/toc/2045-2322Abstract Hematopoietic stem cells (HSCs) in the bone marrow are able to differentiate into all types of blood cells and supply the organism each day with billions of fresh cells. They are applied to cure hematological diseases such as leukemia. The clinical need for HSCs is high and there is a demand for being able to control and multiply HSCs in vitro. The hematopoietic system is highly proliferative and thus sensitive to anti-proliferative drugs such as chemotherapeutics. For many of these drugs suppression of the hematopoietic system is the dose-limiting toxicity. Therefore, biomimetic 3D models of the HSC niche that allow to control HSC behavior in vitro and to test drugs in a human setting are relevant for the clinics and pharmacology. Here, we describe a perfused 3D bone marrow analog that allows mimicking the HSC niche under steady-state and activated conditions that favor either HSC maintenance or differentiation, respectively, and allows for drug testing.Lisa RödlingIvo SchwedhelmSaskia KrausKaren BiebackJan HansmannCornelia Lee-ThedieckNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa Rödling Ivo Schwedhelm Saskia Kraus Karen Bieback Jan Hansmann Cornelia Lee-Thedieck 3D models of the hematopoietic stem cell niche under steady-state and active conditions
description	Abstract Hematopoietic stem cells (HSCs) in the bone marrow are able to differentiate into all types of blood cells and supply the organism each day with billions of fresh cells. They are applied to cure hematological diseases such as leukemia. The clinical need for HSCs is high and there is a demand for being able to control and multiply HSCs in vitro. The hematopoietic system is highly proliferative and thus sensitive to anti-proliferative drugs such as chemotherapeutics. For many of these drugs suppression of the hematopoietic system is the dose-limiting toxicity. Therefore, biomimetic 3D models of the HSC niche that allow to control HSC behavior in vitro and to test drugs in a human setting are relevant for the clinics and pharmacology. Here, we describe a perfused 3D bone marrow analog that allows mimicking the HSC niche under steady-state and activated conditions that favor either HSC maintenance or differentiation, respectively, and allows for drug testing.
format	article
author	Lisa Rödling Ivo Schwedhelm Saskia Kraus Karen Bieback Jan Hansmann Cornelia Lee-Thedieck
author_facet	Lisa Rödling Ivo Schwedhelm Saskia Kraus Karen Bieback Jan Hansmann Cornelia Lee-Thedieck
author_sort	Lisa Rödling
title	3D models of the hematopoietic stem cell niche under steady-state and active conditions
title_short	3D models of the hematopoietic stem cell niche under steady-state and active conditions
title_full	3D models of the hematopoietic stem cell niche under steady-state and active conditions
title_fullStr	3D models of the hematopoietic stem cell niche under steady-state and active conditions
title_full_unstemmed	3D models of the hematopoietic stem cell niche under steady-state and active conditions
title_sort	3d models of the hematopoietic stem cell niche under steady-state and active conditions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4375f52eee9a49e89c5477d4f7a5411c
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_version_	1718394032992813056

3D models of the hematopoietic stem cell niche under steady-state and active conditions

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