Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease.
<h4>Background</h4>The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates...
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oai:doaj.org-article:4378dc395fd44380a19da07c936b79aa2021-11-18T08:22:45ZGenome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease.1932-620310.1371/journal.pone.0095543https://doaj.org/article/4378dc395fd44380a19da07c936b79aa2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24743270/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced.<h4>Methodology/principal findings</h4>Genome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana 'Brazil' in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety 'Brazil'.<h4>Conclusions/significance</h4>Foc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will thus advance us develop effective methods for managing the banana vascular wilt disease, including improvement of disease resistance in banana.Lijia GuoLijuan HanLaying YangHuicai ZengDingding FanYabin ZhuYue FengGuofen WangChunfang PengXuanting JiangDajie ZhouPeixiang NiChangcong LiangLei LiuJun WangChao MaoXiaodong FangMing PengJunsheng HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e95543 (2014) |
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Medicine R Science Q Lijia Guo Lijuan Han Laying Yang Huicai Zeng Dingding Fan Yabin Zhu Yue Feng Guofen Wang Chunfang Peng Xuanting Jiang Dajie Zhou Peixiang Ni Changcong Liang Lei Liu Jun Wang Chao Mao Xiaodong Fang Ming Peng Junsheng Huang Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| description |
<h4>Background</h4>The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced.<h4>Methodology/principal findings</h4>Genome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana 'Brazil' in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety 'Brazil'.<h4>Conclusions/significance</h4>Foc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will thus advance us develop effective methods for managing the banana vascular wilt disease, including improvement of disease resistance in banana. |
| format |
article |
| author |
Lijia Guo Lijuan Han Laying Yang Huicai Zeng Dingding Fan Yabin Zhu Yue Feng Guofen Wang Chunfang Peng Xuanting Jiang Dajie Zhou Peixiang Ni Changcong Liang Lei Liu Jun Wang Chao Mao Xiaodong Fang Ming Peng Junsheng Huang |
| author_facet |
Lijia Guo Lijuan Han Laying Yang Huicai Zeng Dingding Fan Yabin Zhu Yue Feng Guofen Wang Chunfang Peng Xuanting Jiang Dajie Zhou Peixiang Ni Changcong Liang Lei Liu Jun Wang Chao Mao Xiaodong Fang Ming Peng Junsheng Huang |
| author_sort |
Lijia Guo |
| title |
Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| title_short |
Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| title_full |
Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| title_fullStr |
Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| title_full_unstemmed |
Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| title_sort |
genome and transcriptome analysis of the fungal pathogen fusarium oxysporum f. sp. cubense causing banana vascular wilt disease. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/4378dc395fd44380a19da07c936b79aa |
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