Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration

Abstract Epimorphic regeneration of appendages is a complex and complete phenomenon found in selected animals. Hemidactylus frenatus, house gecko has the remarkable ability to regenerate the tail tissue upon autotomy involving epimorphic regeneration mechanism. This study has identified and evaluate...

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Autores principales:	Sai Pawan Nagumantri, Sarena Banu, Mohammed M. Idris
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/437ef4dc9b734a1e8910b86ac258190f
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spelling	oai:doaj.org-article:437ef4dc9b734a1e8910b86ac258190f2021-12-02T13:30:34ZTranscriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration10.1038/s41598-021-83283-02045-2322https://doaj.org/article/437ef4dc9b734a1e8910b86ac258190f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83283-0https://doaj.org/toc/2045-2322Abstract Epimorphic regeneration of appendages is a complex and complete phenomenon found in selected animals. Hemidactylus frenatus, house gecko has the remarkable ability to regenerate the tail tissue upon autotomy involving epimorphic regeneration mechanism. This study has identified and evaluated the molecular changes at gene and protein level during the initial stages, i.e., during the wound healing and repair mechanism initiation stage of tail regeneration. Based on next generation transcriptomics and De novo analysis the transcriptome library of the gecko tail tissue was generated. A total of 254 genes and 128 proteins were found to be associated with the regeneration of gecko tail tissue upon amputation at 1, 2 and 5-day post amputation (dpa) against control, 0-dpa through differential transcriptomic and proteomic analysis. To authenticate the expression analysis, 50 genes were further validated involving RTPCR. 327 genes/proteins identified and mapped from the study showed association for Protein kinase A signaling, Telomerase BAG2 signaling, paxillin signaling, VEGF signaling network pathways based on network pathway analysis. This study empanelled list of transcriptome, proteome and the list of genes/proteins associated with the tail regeneration.Sai Pawan NagumantriSarena BanuMohammed M. IdrisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sai Pawan Nagumantri Sarena Banu Mohammed M. Idris Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration
description	Abstract Epimorphic regeneration of appendages is a complex and complete phenomenon found in selected animals. Hemidactylus frenatus, house gecko has the remarkable ability to regenerate the tail tissue upon autotomy involving epimorphic regeneration mechanism. This study has identified and evaluated the molecular changes at gene and protein level during the initial stages, i.e., during the wound healing and repair mechanism initiation stage of tail regeneration. Based on next generation transcriptomics and De novo analysis the transcriptome library of the gecko tail tissue was generated. A total of 254 genes and 128 proteins were found to be associated with the regeneration of gecko tail tissue upon amputation at 1, 2 and 5-day post amputation (dpa) against control, 0-dpa through differential transcriptomic and proteomic analysis. To authenticate the expression analysis, 50 genes were further validated involving RTPCR. 327 genes/proteins identified and mapped from the study showed association for Protein kinase A signaling, Telomerase BAG2 signaling, paxillin signaling, VEGF signaling network pathways based on network pathway analysis. This study empanelled list of transcriptome, proteome and the list of genes/proteins associated with the tail regeneration.
format	article
author	Sai Pawan Nagumantri Sarena Banu Mohammed M. Idris
author_facet	Sai Pawan Nagumantri Sarena Banu Mohammed M. Idris
author_sort	Sai Pawan Nagumantri
title	Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration
title_short	Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration
title_full	Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration
title_fullStr	Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration
title_full_unstemmed	Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration
title_sort	transcriptomic and proteomic analysis of hemidactylus frenatus during initial stages of tail regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/437ef4dc9b734a1e8910b86ac258190f
work_keys_str_mv	AT saipawannagumantri transcriptomicandproteomicanalysisofhemidactylusfrenatusduringinitialstagesoftailregeneration AT sarenabanu transcriptomicandproteomicanalysisofhemidactylusfrenatusduringinitialstagesoftailregeneration AT mohammedmidris transcriptomicandproteomicanalysisofhemidactylusfrenatusduringinitialstagesoftailregeneration
_version_	1718392910461796352

Transcriptomic and proteomic analysis of Hemidactylus frenatus during initial stages of tail regeneration

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