Single cell profiling of capillary blood enables out of clinic human immunity studies

Abstract An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequ...

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Auteurs principaux:	Tatyana Dobreva, David Brown, Jong Hwee Park, Matt Thomson
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2020
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/4385e7f479a54649bcbd4e5bf2d7da6b
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spelling	oai:doaj.org-article:4385e7f479a54649bcbd4e5bf2d7da6b2021-12-02T16:08:58ZSingle cell profiling of capillary blood enables out of clinic human immunity studies10.1038/s41598-020-77073-32045-2322https://doaj.org/article/4385e7f479a54649bcbd4e5bf2d7da6b2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77073-3https://doaj.org/toc/2045-2322Abstract An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.Tatyana DobrevaDavid BrownJong Hwee ParkMatt ThomsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatyana Dobreva David Brown Jong Hwee Park Matt Thomson Single cell profiling of capillary blood enables out of clinic human immunity studies
description	Abstract An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.
format	article
author	Tatyana Dobreva David Brown Jong Hwee Park Matt Thomson
author_facet	Tatyana Dobreva David Brown Jong Hwee Park Matt Thomson
author_sort	Tatyana Dobreva
title	Single cell profiling of capillary blood enables out of clinic human immunity studies
title_short	Single cell profiling of capillary blood enables out of clinic human immunity studies
title_full	Single cell profiling of capillary blood enables out of clinic human immunity studies
title_fullStr	Single cell profiling of capillary blood enables out of clinic human immunity studies
title_full_unstemmed	Single cell profiling of capillary blood enables out of clinic human immunity studies
title_sort	single cell profiling of capillary blood enables out of clinic human immunity studies
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4385e7f479a54649bcbd4e5bf2d7da6b
work_keys_str_mv	AT tatyanadobreva singlecellprofilingofcapillarybloodenablesoutofclinichumanimmunitystudies AT davidbrown singlecellprofilingofcapillarybloodenablesoutofclinichumanimmunitystudies AT jonghweepark singlecellprofilingofcapillarybloodenablesoutofclinichumanimmunitystudies AT mattthomson singlecellprofilingofcapillarybloodenablesoutofclinichumanimmunitystudies
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Single cell profiling of capillary blood enables out of clinic human immunity studies

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