Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1

ABSTRACT Recent application of mutation accumulation techniques combined with whole-genome sequencing (MA/WGS) has greatly promoted studies of spontaneous mutation. However, such explorations have rarely been conducted on marine organisms, and it is unclear how marine habitats have influenced genome...

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Autores principales:	Chloe Strauss, Hongan Long, Caitlyn E. Patterson, Ronald Te, Michael Lynch
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	environmental dependence of mutations evolutionary genomics mutation accumulation neutral evolution Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/438dbd45b0624f5088f0ad3f01f9a63d
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spelling	oai:doaj.org-article:438dbd45b0624f5088f0ad3f01f9a63d2021-11-15T15:51:43ZGenome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK110.1128/mBio.01021-172150-7511https://doaj.org/article/438dbd45b0624f5088f0ad3f01f9a63d2017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01021-17https://doaj.org/toc/2150-7511ABSTRACT Recent application of mutation accumulation techniques combined with whole-genome sequencing (MA/WGS) has greatly promoted studies of spontaneous mutation. However, such explorations have rarely been conducted on marine organisms, and it is unclear how marine habitats have influenced genome stability. This report resolves the mutation rate and spectrum of the coral reef pathogen Vibrio shilonii, which causes coral bleaching and endangers the biodiversity maintained by coral reefs. We found that its mutation rate and spectrum are highly similar to those of other studied bacteria from various habitats, despite the saline environment. The mutational properties of this marine bacterium are thus controlled by other general evolutionary forces such as natural selection and genetic drift. We also found that as pH drops, the mutation rate decreases and the mutation spectrum is biased in the direction of generating G/C nucleotides. This implies that evolutionary features of this organism and perhaps other marine microbes might be altered by the increasingly acidic ocean water caused by excess CO2 emission. Nonetheless, further exploration is needed as the pH range tested in this study was rather narrow and many other possible mutation determinants, such as carbonate increase, are associated with ocean acidification. IMPORTANCE This study explored the pH dependence of a bacterial genome-wide mutation rate. We discovered that the genome-wide rates of appearance of most mutation types decrease linearly and that the mutation spectrum is biased in generating more G/C nucleotides with pH drop in the coral reef pathogen V. shilonii.Chloe StraussHongan LongCaitlyn E. PattersonRonald TeMichael LynchAmerican Society for Microbiologyarticleenvironmental dependence of mutationsevolutionary genomicsmutation accumulationneutral evolutionMicrobiologyQR1-502ENmBio, Vol 8, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	environmental dependence of mutations evolutionary genomics mutation accumulation neutral evolution Microbiology QR1-502
spellingShingle	environmental dependence of mutations evolutionary genomics mutation accumulation neutral evolution Microbiology QR1-502 Chloe Strauss Hongan Long Caitlyn E. Patterson Ronald Te Michael Lynch Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1
description	ABSTRACT Recent application of mutation accumulation techniques combined with whole-genome sequencing (MA/WGS) has greatly promoted studies of spontaneous mutation. However, such explorations have rarely been conducted on marine organisms, and it is unclear how marine habitats have influenced genome stability. This report resolves the mutation rate and spectrum of the coral reef pathogen Vibrio shilonii, which causes coral bleaching and endangers the biodiversity maintained by coral reefs. We found that its mutation rate and spectrum are highly similar to those of other studied bacteria from various habitats, despite the saline environment. The mutational properties of this marine bacterium are thus controlled by other general evolutionary forces such as natural selection and genetic drift. We also found that as pH drops, the mutation rate decreases and the mutation spectrum is biased in the direction of generating G/C nucleotides. This implies that evolutionary features of this organism and perhaps other marine microbes might be altered by the increasingly acidic ocean water caused by excess CO2 emission. Nonetheless, further exploration is needed as the pH range tested in this study was rather narrow and many other possible mutation determinants, such as carbonate increase, are associated with ocean acidification. IMPORTANCE This study explored the pH dependence of a bacterial genome-wide mutation rate. We discovered that the genome-wide rates of appearance of most mutation types decrease linearly and that the mutation spectrum is biased in generating more G/C nucleotides with pH drop in the coral reef pathogen V. shilonii.
format	article
author	Chloe Strauss Hongan Long Caitlyn E. Patterson Ronald Te Michael Lynch
author_facet	Chloe Strauss Hongan Long Caitlyn E. Patterson Ronald Te Michael Lynch
author_sort	Chloe Strauss
title	Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1
title_short	Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1
title_full	Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1
title_fullStr	Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1
title_full_unstemmed	Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1
title_sort	genome-wide mutation rate response to ph change in the coral reef pathogen <italic toggle="yes">vibrio shilonii</italic> ak1
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/438dbd45b0624f5088f0ad3f01f9a63d
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Genome-Wide Mutation Rate Response to pH Change in the Coral Reef Pathogen <italic toggle="yes">Vibrio shilonii</italic> AK1

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