Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker

Salwa Aldahlawi,1 Abdel-Rahman Youssef,1 Syed Shahabuddin2,3 1Department of Basic and Clinical Oral Sciences, College of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia; 2Abbvie, North Chicago, IL,USA; 3Deportment of Biology, City Colleges of Chicago, Chicago, IL, USA Purpose: The aim of thi...

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Autores principales:	Aldahlawi S, Youssef AR, Shahabuddin S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Periodontal disease alveolar bone loss gingival crevicular fluid saliva serum biomarker CXCL10 IP-10 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/438e13fd08c74d13a3bc4d73fd18599d
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spelling	oai:doaj.org-article:438e13fd08c74d13a3bc4d73fd18599d2021-12-02T02:21:29ZEvaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker1178-7031https://doaj.org/article/438e13fd08c74d13a3bc4d73fd18599d2018-10-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-chemokine-cxcl10-in-human-gingival-crevicular-fluid-sali-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Salwa Aldahlawi,1 Abdel-Rahman Youssef,1 Syed Shahabuddin2,3 1Department of Basic and Clinical Oral Sciences, College of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia; 2Abbvie, North Chicago, IL,USA; 3Deportment of Biology, City Colleges of Chicago, Chicago, IL, USA Purpose: The aim of this study was to evaluate CXCL10 as a biomarker for periodontitis by determining the CXCL10 levels in saliva, serum, and gingival crevicular fluid (GCF) samples from periodontally healthy control subjects and adult subjects with chronic periodontitis.Patients and methods: Adult patients seeking dental treatment at Umm Al-Qura University dental clinic underwent a complete periodontal examination, and saliva, serum, and GCF samples were collected. Subjects were classified as chronic periodontitis patients (n=31) if they have a periodontal probing depth (PD) of ≥4 mm and/or clinical attachment level (CAL) of ≥3 mm in >30% of the teeth. The control group (n=25) had PD ≤3 mm and/or CAL ≤2 mm. ELISA was performed to determine the concentration of CXCL10 in saliva, serum, and GCF samples. Student’s t-test was carried out to evaluate the significant difference between different groups. Spearman’s correlation test was used to analyze the relationship between the levels of CXCL10 and the clinical periodontal parameters. P-value of ≤0.05 was considered significant.Results: Significantly higher concentrations of CXCL10 were found in saliva and serum in chronic periodontitis patients as compared with the controls (272±60.4 pg/mL and 72±13.4 pg/mL vs 130±22.2 pg/mL and 44.08±4.5 pg/mL, P≤0.05). The CXCL10 levels in GCF were higher in the periodontitis group as compared with the control group (66.36±32.0 pg/mL and 44.56±17.5 pg/mL, respectively); the difference did not reach statistical significance (P≥0.05). Moreover, serum CXCL10 level was significantly higher in periodontitis patients with moderate to severe bone loss as compared with those with mild bone loss (71.05±4.7 pg/mL vs 54.8±7.7 pg/mL, P≤0.05). The serum CXCL10 levels were found to be related to CAL measurements (r=0.3, P=0.026), while the saliva CXCL10 levels were related to PD measurements (r=0.8, P=0.0007).Conclusion: CXCL10 is significantly increased in periodontitis subjects as compared with controls and could be used as a marker for periodontal disease. Keywords: periodontal disease, alveolar bone loss, gingival crevicular fluid, saliva, serum, biomarker, CXCL10, IP-10Aldahlawi SYoussef ARShahabuddin SDove Medical PressarticlePeriodontal diseasealveolar bone lossgingival crevicular fluidsalivaserumbiomarkerCXCL10IP-10PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 11, Pp 389-396 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Periodontal disease alveolar bone loss gingival crevicular fluid saliva serum biomarker CXCL10 IP-10 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Periodontal disease alveolar bone loss gingival crevicular fluid saliva serum biomarker CXCL10 IP-10 Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Aldahlawi S Youssef AR Shahabuddin S Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
description	Salwa Aldahlawi,1 Abdel-Rahman Youssef,1 Syed Shahabuddin2,3 1Department of Basic and Clinical Oral Sciences, College of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia; 2Abbvie, North Chicago, IL,USA; 3Deportment of Biology, City Colleges of Chicago, Chicago, IL, USA Purpose: The aim of this study was to evaluate CXCL10 as a biomarker for periodontitis by determining the CXCL10 levels in saliva, serum, and gingival crevicular fluid (GCF) samples from periodontally healthy control subjects and adult subjects with chronic periodontitis.Patients and methods: Adult patients seeking dental treatment at Umm Al-Qura University dental clinic underwent a complete periodontal examination, and saliva, serum, and GCF samples were collected. Subjects were classified as chronic periodontitis patients (n=31) if they have a periodontal probing depth (PD) of ≥4 mm and/or clinical attachment level (CAL) of ≥3 mm in >30% of the teeth. The control group (n=25) had PD ≤3 mm and/or CAL ≤2 mm. ELISA was performed to determine the concentration of CXCL10 in saliva, serum, and GCF samples. Student’s t-test was carried out to evaluate the significant difference between different groups. Spearman’s correlation test was used to analyze the relationship between the levels of CXCL10 and the clinical periodontal parameters. P-value of ≤0.05 was considered significant.Results: Significantly higher concentrations of CXCL10 were found in saliva and serum in chronic periodontitis patients as compared with the controls (272±60.4 pg/mL and 72±13.4 pg/mL vs 130±22.2 pg/mL and 44.08±4.5 pg/mL, P≤0.05). The CXCL10 levels in GCF were higher in the periodontitis group as compared with the control group (66.36±32.0 pg/mL and 44.56±17.5 pg/mL, respectively); the difference did not reach statistical significance (P≥0.05). Moreover, serum CXCL10 level was significantly higher in periodontitis patients with moderate to severe bone loss as compared with those with mild bone loss (71.05±4.7 pg/mL vs 54.8±7.7 pg/mL, P≤0.05). The serum CXCL10 levels were found to be related to CAL measurements (r=0.3, P=0.026), while the saliva CXCL10 levels were related to PD measurements (r=0.8, P=0.0007).Conclusion: CXCL10 is significantly increased in periodontitis subjects as compared with controls and could be used as a marker for periodontal disease. Keywords: periodontal disease, alveolar bone loss, gingival crevicular fluid, saliva, serum, biomarker, CXCL10, IP-10
format	article
author	Aldahlawi S Youssef AR Shahabuddin S
author_facet	Aldahlawi S Youssef AR Shahabuddin S
author_sort	Aldahlawi S
title	Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
title_short	Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
title_full	Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
title_fullStr	Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
title_full_unstemmed	Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
title_sort	evaluation of chemokine cxcl10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/438e13fd08c74d13a3bc4d73fd18599d
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Evaluation of chemokine CXCL10 in human gingival crevicular fluid, saliva, and serum as periodontitis biomarker

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