Parental age and gene expression profiles in individual human blastocysts

Abstract The epigenetic status of the genome changes dynamically from fertilization to implantation. In addition, the physiological environment during the process of gametogenesis, including parental age, may affect the epigenome of the embryo after fertilization. It is important to clarify the infl...

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Autores principales:	Kiyotaka Kawai, Tatsuya Harada, Tomonori Ishikawa, Rikikazu Sugiyama, Toshihiro Kawamura, Atsumi Yoshida, Osamu Tsutsumi, Fumitoshi Ishino, Toshiro Kubota, Takashi Kohda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43936361f25441f9a9353dce2212dad2
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spelling	oai:doaj.org-article:43936361f25441f9a9353dce2212dad22021-12-02T15:07:50ZParental age and gene expression profiles in individual human blastocysts10.1038/s41598-018-20614-82045-2322https://doaj.org/article/43936361f25441f9a9353dce2212dad22018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20614-8https://doaj.org/toc/2045-2322Abstract The epigenetic status of the genome changes dynamically from fertilization to implantation. In addition, the physiological environment during the process of gametogenesis, including parental age, may affect the epigenome of the embryo after fertilization. It is important to clarify the influence of parental age on gene expression in the embryo in terms of transgenerational epigenetics to improve the techniques currently used in assisted reproductive medicine. Here, we performed single-embryo RNA-seq analysis on human blastocysts fertilized by intracytoplasmic sperm injection, including from relatively elderly mothers, to elucidate the effects of parental age on the embryonic gene expression profile. We identified a number of genes in which the expression levels were decreased with increasing maternal age. Among these genes, several are considered to be important for meiotic chromosomal segregation, such as PTTG1, AURKC, SMC1B and MEIKIN. Furthermore, the expression levels of certain genes critical for autophagy and embryonic growth, specifically GABARAPL1 and GABARAPL3, were negatively correlated with advanced paternal age. In addition, levels of transcripts derived from major satellite repeats also decreased as the maternal age increased. These results suggest that epigenetic modifications of the oocyte genome may change with parental age and be transmitted to the next generation.Kiyotaka KawaiTatsuya HaradaTomonori IshikawaRikikazu SugiyamaToshihiro KawamuraAtsumi YoshidaOsamu TsutsumiFumitoshi IshinoToshiro KubotaTakashi KohdaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kiyotaka Kawai Tatsuya Harada Tomonori Ishikawa Rikikazu Sugiyama Toshihiro Kawamura Atsumi Yoshida Osamu Tsutsumi Fumitoshi Ishino Toshiro Kubota Takashi Kohda Parental age and gene expression profiles in individual human blastocysts
description	Abstract The epigenetic status of the genome changes dynamically from fertilization to implantation. In addition, the physiological environment during the process of gametogenesis, including parental age, may affect the epigenome of the embryo after fertilization. It is important to clarify the influence of parental age on gene expression in the embryo in terms of transgenerational epigenetics to improve the techniques currently used in assisted reproductive medicine. Here, we performed single-embryo RNA-seq analysis on human blastocysts fertilized by intracytoplasmic sperm injection, including from relatively elderly mothers, to elucidate the effects of parental age on the embryonic gene expression profile. We identified a number of genes in which the expression levels were decreased with increasing maternal age. Among these genes, several are considered to be important for meiotic chromosomal segregation, such as PTTG1, AURKC, SMC1B and MEIKIN. Furthermore, the expression levels of certain genes critical for autophagy and embryonic growth, specifically GABARAPL1 and GABARAPL3, were negatively correlated with advanced paternal age. In addition, levels of transcripts derived from major satellite repeats also decreased as the maternal age increased. These results suggest that epigenetic modifications of the oocyte genome may change with parental age and be transmitted to the next generation.
format	article
author	Kiyotaka Kawai Tatsuya Harada Tomonori Ishikawa Rikikazu Sugiyama Toshihiro Kawamura Atsumi Yoshida Osamu Tsutsumi Fumitoshi Ishino Toshiro Kubota Takashi Kohda
author_facet	Kiyotaka Kawai Tatsuya Harada Tomonori Ishikawa Rikikazu Sugiyama Toshihiro Kawamura Atsumi Yoshida Osamu Tsutsumi Fumitoshi Ishino Toshiro Kubota Takashi Kohda
author_sort	Kiyotaka Kawai
title	Parental age and gene expression profiles in individual human blastocysts
title_short	Parental age and gene expression profiles in individual human blastocysts
title_full	Parental age and gene expression profiles in individual human blastocysts
title_fullStr	Parental age and gene expression profiles in individual human blastocysts
title_full_unstemmed	Parental age and gene expression profiles in individual human blastocysts
title_sort	parental age and gene expression profiles in individual human blastocysts
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/43936361f25441f9a9353dce2212dad2
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_version_	1718388380568387584

Parental age and gene expression profiles in individual human blastocysts

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