DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS

Already about one hundred years BCG remains the only widely used tuberculosis (TB) vaccine. It is known that intensity of the BCG-induced Th1 immune response decreases over time and comes to naught within 10-15 years. It significantly distinguishes BCG from the vaccines providing a lifelong protecti...

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Autores principales:	V. V. Yeremeev, I. V. Dukhovlinov, A. I. Orlov, G. S. Shepelkova, E. A. Fedorova, M. B. Balazovsky, V. Ya. Gergert
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	tuberculosis vaccine immune memory Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/43957476b69644db91835395497686c3
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spelling	oai:doaj.org-article:43957476b69644db91835395497686c32021-11-18T08:03:47ZDURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS1563-06252313-741X10.15789/1563-0625-2018-2-271-276https://doaj.org/article/43957476b69644db91835395497686c32018-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1483https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAlready about one hundred years BCG remains the only widely used tuberculosis (TB) vaccine. It is known that intensity of the BCG-induced Th1 immune response decreases over time and comes to naught within 10-15 years. It significantly distinguishes BCG from the vaccines providing a lifelong protection such as vaccines against poliomyelitis or measles, and can be bound to natural restriction of duration of a persistence of the BCG-induced CD4+ T-cells. Data on insufficient ability of BCG to stimulate life-long immunological memory is accumulating. Earlier in our lab on the model of rather resistant to TB C57BL/6 mice infection with the virulent laboratory strain of Mycobacterium tuberculosis (Mtb) H37Rv protective activity (comparable to that of BCG Russia) of 3 subunit vaccine variants was demonstrated as assessed by lung and spleen CFU counts and life span of animals after infection. The aim of this study was to study the characteristics and duration of the immune response induced by the most effective variant of these vaccines. Groups of C57BL/6 mice were vaccinated intramuscularly twice with two-week intervals with 10 μg protein conjugated to 200 μl of an aluminum hydroxide emulsion. Immune response (production of specific antibodies, vaccine proteinstimulated production of interferon gamma and proliferation in vitro) was monitored during 10 months after vaccination. We have shown that the test vaccine induces in mice the formation of long-term immunological memory to a bacterial antigen. Moreover, in the presence of glutoxim the immunological memory spectrum shifts to a "protective" type, i.e. the predominance of the cellular component of the immune response over the antibody response is stimulated. The next step will be the investigation of vaccine effectiveness for revaccination after primary BCG immunization.V. V. YeremeevI. V. DukhovlinovA. I. OrlovG. S. ShepelkovaE. A. FedorovaM. B. BalazovskyV. Ya. GergertSPb RAACIarticletuberculosisvaccineimmune memoryImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 2, Pp 271-276 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	tuberculosis vaccine immune memory Immunologic diseases. Allergy RC581-607
spellingShingle	tuberculosis vaccine immune memory Immunologic diseases. Allergy RC581-607 V. V. Yeremeev I. V. Dukhovlinov A. I. Orlov G. S. Shepelkova E. A. Fedorova M. B. Balazovsky V. Ya. Gergert DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS
description	Already about one hundred years BCG remains the only widely used tuberculosis (TB) vaccine. It is known that intensity of the BCG-induced Th1 immune response decreases over time and comes to naught within 10-15 years. It significantly distinguishes BCG from the vaccines providing a lifelong protection such as vaccines against poliomyelitis or measles, and can be bound to natural restriction of duration of a persistence of the BCG-induced CD4+ T-cells. Data on insufficient ability of BCG to stimulate life-long immunological memory is accumulating. Earlier in our lab on the model of rather resistant to TB C57BL/6 mice infection with the virulent laboratory strain of Mycobacterium tuberculosis (Mtb) H37Rv protective activity (comparable to that of BCG Russia) of 3 subunit vaccine variants was demonstrated as assessed by lung and spleen CFU counts and life span of animals after infection. The aim of this study was to study the characteristics and duration of the immune response induced by the most effective variant of these vaccines. Groups of C57BL/6 mice were vaccinated intramuscularly twice with two-week intervals with 10 μg protein conjugated to 200 μl of an aluminum hydroxide emulsion. Immune response (production of specific antibodies, vaccine proteinstimulated production of interferon gamma and proliferation in vitro) was monitored during 10 months after vaccination. We have shown that the test vaccine induces in mice the formation of long-term immunological memory to a bacterial antigen. Moreover, in the presence of glutoxim the immunological memory spectrum shifts to a "protective" type, i.e. the predominance of the cellular component of the immune response over the antibody response is stimulated. The next step will be the investigation of vaccine effectiveness for revaccination after primary BCG immunization.
format	article
author	V. V. Yeremeev I. V. Dukhovlinov A. I. Orlov G. S. Shepelkova E. A. Fedorova M. B. Balazovsky V. Ya. Gergert
author_facet	V. V. Yeremeev I. V. Dukhovlinov A. I. Orlov G. S. Shepelkova E. A. Fedorova M. B. Balazovsky V. Ya. Gergert
author_sort	V. V. Yeremeev
title	DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS
title_short	DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS
title_full	DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS
title_fullStr	DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS
title_full_unstemmed	DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS
title_sort	duration of immune response induced by the vaccine based on recombinant ag85, tb10 and flic proteins
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/43957476b69644db91835395497686c3
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DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS

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