Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.

The application of active dry yeast (ADY) in bioethanol production simplifies operation processes and reduces the risk of bacterial contamination. In the present study, we constructed a novel ADY strain with improved stress tolerance and ethanol fermentation performances under stressful conditions....

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Autores principales:	Daoqiong Zheng, Ke Zhang, Kehui Gao, Zewei Liu, Xing Zhang, Ou Li, Jianguo Sun, Xiaoyang Zhang, Fengguang Du, Peiyong Sun, Aimin Qu, Xuechang Wu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4398c8edbeee46babcd20c8ea0f771f1
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spelling	oai:doaj.org-article:4398c8edbeee46babcd20c8ea0f771f12021-11-18T08:40:28ZConstruction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.1932-620310.1371/journal.pone.0085022https://doaj.org/article/4398c8edbeee46babcd20c8ea0f771f12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376860/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The application of active dry yeast (ADY) in bioethanol production simplifies operation processes and reduces the risk of bacterial contamination. In the present study, we constructed a novel ADY strain with improved stress tolerance and ethanol fermentation performances under stressful conditions. The industrial Saccharomyces cerevisiae strain ZTW1 showed excellent properties and thus subjected to a modified whole-genome shuffling (WGS) process to improve its ethanol titer, proliferation capability, and multiple stress tolerance for ADY production. The best-performing mutant, Z3-86, was obtained after three rounds of WGS, producing 4.4% more ethanol and retaining 2.15-fold higher viability than ZTW1 after drying. Proteomics and physiological analyses indicated that the altered expression patterns of genes involved in protein metabolism, plasma membrane composition, trehalose metabolism, and oxidative responses contribute to the trait improvement of Z3-86. This work not only successfully developed a novel S. cerevisiae mutant for application in commercial bioethanol production, but also enriched the current understanding of how WGS improves the complex traits of microbes.Daoqiong ZhengKe ZhangKehui GaoZewei LiuXing ZhangOu LiJianguo SunXiaoyang ZhangFengguang DuPeiyong SunAimin QuXuechang WuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e85022 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daoqiong Zheng Ke Zhang Kehui Gao Zewei Liu Xing Zhang Ou Li Jianguo Sun Xiaoyang Zhang Fengguang Du Peiyong Sun Aimin Qu Xuechang Wu Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
description	The application of active dry yeast (ADY) in bioethanol production simplifies operation processes and reduces the risk of bacterial contamination. In the present study, we constructed a novel ADY strain with improved stress tolerance and ethanol fermentation performances under stressful conditions. The industrial Saccharomyces cerevisiae strain ZTW1 showed excellent properties and thus subjected to a modified whole-genome shuffling (WGS) process to improve its ethanol titer, proliferation capability, and multiple stress tolerance for ADY production. The best-performing mutant, Z3-86, was obtained after three rounds of WGS, producing 4.4% more ethanol and retaining 2.15-fold higher viability than ZTW1 after drying. Proteomics and physiological analyses indicated that the altered expression patterns of genes involved in protein metabolism, plasma membrane composition, trehalose metabolism, and oxidative responses contribute to the trait improvement of Z3-86. This work not only successfully developed a novel S. cerevisiae mutant for application in commercial bioethanol production, but also enriched the current understanding of how WGS improves the complex traits of microbes.
format	article
author	Daoqiong Zheng Ke Zhang Kehui Gao Zewei Liu Xing Zhang Ou Li Jianguo Sun Xiaoyang Zhang Fengguang Du Peiyong Sun Aimin Qu Xuechang Wu
author_facet	Daoqiong Zheng Ke Zhang Kehui Gao Zewei Liu Xing Zhang Ou Li Jianguo Sun Xiaoyang Zhang Fengguang Du Peiyong Sun Aimin Qu Xuechang Wu
author_sort	Daoqiong Zheng
title	Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
title_short	Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
title_full	Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
title_fullStr	Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
title_full_unstemmed	Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.
title_sort	construction of novel saccharomyces cerevisiae strains for bioethanol active dry yeast (ady) production.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4398c8edbeee46babcd20c8ea0f771f1
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Construction of novel Saccharomyces cerevisiae strains for bioethanol active dry yeast (ADY) production.

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