Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing

Common dab Limanda limanda from Scottish and international waters were examined by quantitative real-time RT-qPCR for evidence of viral RNA consistent with salmonid alphaviruses (SAV). SAV prevalence in heart tissue varied between sampling sites and reached up to 17% in fish collected near the Shetl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	DW Bruno, PA Noguera, J Black, W Murray, DJ Macqueen, I Matejusova
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2014
Materias:	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/439950b7d8494e8583d6977d453c7378
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:439950b7d8494e8583d6977d453c7378
record_format	dspace
spelling	oai:doaj.org-article:439950b7d8494e8583d6977d453c73782021-11-17T10:08:24ZIdentification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing1869-215X1869-753410.3354/aei00097https://doaj.org/article/439950b7d8494e8583d6977d453c73782014-04-01T00:00:00Zhttps://www.int-res.com/abstracts/aei/v5/n1/p89-98/https://doaj.org/toc/1869-215Xhttps://doaj.org/toc/1869-7534Common dab Limanda limanda from Scottish and international waters were examined by quantitative real-time RT-qPCR for evidence of viral RNA consistent with salmonid alphaviruses (SAV). SAV prevalence in heart tissue varied between sampling sites and reached up to 17% in fish collected near the Shetland Islands, Scotland. Raw Ct values ranging from 22.31 to 39.45 were obtained from the SAV-positive tissue material using the nsP1 RT-qPCR assay. Bayesian-, likelihood- and distance-based phylogenetic analyses performed with the amplified partial E2 gene sequence dataset suggest that the dab-derived virus belongs to SAV Subtypes I, II and V. A single SAV subtype was identified from the majority of sampling sites, apart from Shetland, where Subtypes II and V were also identified. The presence of SAV RNA from common dab in regions detached from salmon aquaculture lends support to the hypothesis that common dab are bone fide wild reservoirs of SAV, independent of fish farming activity. There was no link between the occurrence of viral RNA, length and sex of the dab, water depth, or health status as recorded using the International Council for the Exploration of the Sea (ICES) guidelines. In addition, the histological changes recorded in dab could not, with certainty, be attributed to infection with SAV. Finally, and for the first time, this study demonstrated that the dab-derived SAV Subtype V virus could be successfully cultured in a salmonid cell line.DW BrunoPA NogueraJ BlackW MurrayDJ MacqueenI MatejusovaInter-ResearcharticleAquaculture. Fisheries. AnglingSH1-691EcologyQH540-549.5ENAquaculture Environment Interactions, Vol 5, Iss 1, Pp 89-98 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5
spellingShingle	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 DW Bruno PA Noguera J Black W Murray DJ Macqueen I Matejusova Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing
description	Common dab Limanda limanda from Scottish and international waters were examined by quantitative real-time RT-qPCR for evidence of viral RNA consistent with salmonid alphaviruses (SAV). SAV prevalence in heart tissue varied between sampling sites and reached up to 17% in fish collected near the Shetland Islands, Scotland. Raw Ct values ranging from 22.31 to 39.45 were obtained from the SAV-positive tissue material using the nsP1 RT-qPCR assay. Bayesian-, likelihood- and distance-based phylogenetic analyses performed with the amplified partial E2 gene sequence dataset suggest that the dab-derived virus belongs to SAV Subtypes I, II and V. A single SAV subtype was identified from the majority of sampling sites, apart from Shetland, where Subtypes II and V were also identified. The presence of SAV RNA from common dab in regions detached from salmon aquaculture lends support to the hypothesis that common dab are bone fide wild reservoirs of SAV, independent of fish farming activity. There was no link between the occurrence of viral RNA, length and sex of the dab, water depth, or health status as recorded using the International Council for the Exploration of the Sea (ICES) guidelines. In addition, the histological changes recorded in dab could not, with certainty, be attributed to infection with SAV. Finally, and for the first time, this study demonstrated that the dab-derived SAV Subtype V virus could be successfully cultured in a salmonid cell line.
format	article
author	DW Bruno PA Noguera J Black W Murray DJ Macqueen I Matejusova
author_facet	DW Bruno PA Noguera J Black W Murray DJ Macqueen I Matejusova
author_sort	DW Bruno
title	Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing
title_short	Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing
title_full	Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing
title_fullStr	Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing
title_full_unstemmed	Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing
title_sort	identification of a wild reservoir of salmonid alphavirus in common dab limanda limanda, with emphasis on virus culture and sequencing
publisher	Inter-Research
publishDate	2014
url	https://doaj.org/article/439950b7d8494e8583d6977d453c7378
work_keys_str_mv	AT dwbruno identificationofawildreservoirofsalmonidalphavirusincommondablimandalimandawithemphasisonviruscultureandsequencing AT panoguera identificationofawildreservoirofsalmonidalphavirusincommondablimandalimandawithemphasisonviruscultureandsequencing AT jblack identificationofawildreservoirofsalmonidalphavirusincommondablimandalimandawithemphasisonviruscultureandsequencing AT wmurray identificationofawildreservoirofsalmonidalphavirusincommondablimandalimandawithemphasisonviruscultureandsequencing AT djmacqueen identificationofawildreservoirofsalmonidalphavirusincommondablimandalimandawithemphasisonviruscultureandsequencing AT imatejusova identificationofawildreservoirofsalmonidalphavirusincommondablimandalimandawithemphasisonviruscultureandsequencing
_version_	1718425606671040512

Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing

Ejemplares similares