The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.

There is a lack in our current understanding on the putative interactions of species of the phyla of Acidobacteria and Verrucomicrobia with plants. Moreover, progress in this area is seriously hampered by the recalcitrance of members of these phyla to grow as pure cultures. The purpose of this study...

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Autores principales:	Ulisses Nunes da Rocha, Caroline M Plugge, Isabelle George, Jan Dirk van Elsas, Leonard Simon van Overbeek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43a196195d414822810abffca4374a96
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spelling	oai:doaj.org-article:43a196195d414822810abffca4374a962021-11-18T08:42:02ZThe rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.1932-620310.1371/journal.pone.0082443https://doaj.org/article/43a196195d414822810abffca4374a962013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349285/?tool=EBIhttps://doaj.org/toc/1932-6203There is a lack in our current understanding on the putative interactions of species of the phyla of Acidobacteria and Verrucomicrobia with plants. Moreover, progress in this area is seriously hampered by the recalcitrance of members of these phyla to grow as pure cultures. The purpose of this study was to investigate whether particular members of Acidobacteria and Verrucomicrobia are avid colonizers of the rhizosphere. Based on previous work, rhizosphere competence was demonstrated for the Verrucomicrobia subdivision 1 groups of Luteolibacter and Candidatus genus Rhizospheria and it was hypothesized that the rhizosphere is a common habitat for Acidobacteria subdivision 8 (class Holophagae). We assessed the population densities of Bacteria, Verrucomicrobia subdivision 1 groups Luteolibacter and Candidatus genus Rhizospheria and Acidobacteria subdivisions 1, 3, 4, 6 and Holophagae in bulk soil and in the rhizospheres of grass, potato and leek in the same field at different points in time using real-time quantitative PCR. Primers of all seven verrucomicrobial, acidobacterial and holophagal PCR systems were based on 16S rRNA gene sequences of cultivable representatives of the different groups. Luteolibacter, Candidatus genus Rhizospheria, subdivision 6 acidobacteria and Holophaga showed preferences for one or more rhizospheres. In particular, the Holophaga 16S rRNA gene number were more abundant in the leek rhizosphere than in bulk soil and the rhizospheres of grass and potato. Attraction to, and colonization of, leek roots by Holophagae strain CHC25 was further shown in an experimental microcosm set-up. In the light of this remarkable capacity, we propose to coin strain CHC25 Candidatus Porrumbacterium oxyphilus (class Holophagae, Phylum Acidobacteria), the first cultured representative with rhizosphere competence.Ulisses Nunes da RochaCaroline M PluggeIsabelle GeorgeJan Dirk van ElsasLeonard Simon van OverbeekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82443 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ulisses Nunes da Rocha Caroline M Plugge Isabelle George Jan Dirk van Elsas Leonard Simon van Overbeek The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
description	There is a lack in our current understanding on the putative interactions of species of the phyla of Acidobacteria and Verrucomicrobia with plants. Moreover, progress in this area is seriously hampered by the recalcitrance of members of these phyla to grow as pure cultures. The purpose of this study was to investigate whether particular members of Acidobacteria and Verrucomicrobia are avid colonizers of the rhizosphere. Based on previous work, rhizosphere competence was demonstrated for the Verrucomicrobia subdivision 1 groups of Luteolibacter and Candidatus genus Rhizospheria and it was hypothesized that the rhizosphere is a common habitat for Acidobacteria subdivision 8 (class Holophagae). We assessed the population densities of Bacteria, Verrucomicrobia subdivision 1 groups Luteolibacter and Candidatus genus Rhizospheria and Acidobacteria subdivisions 1, 3, 4, 6 and Holophagae in bulk soil and in the rhizospheres of grass, potato and leek in the same field at different points in time using real-time quantitative PCR. Primers of all seven verrucomicrobial, acidobacterial and holophagal PCR systems were based on 16S rRNA gene sequences of cultivable representatives of the different groups. Luteolibacter, Candidatus genus Rhizospheria, subdivision 6 acidobacteria and Holophaga showed preferences for one or more rhizospheres. In particular, the Holophaga 16S rRNA gene number were more abundant in the leek rhizosphere than in bulk soil and the rhizospheres of grass and potato. Attraction to, and colonization of, leek roots by Holophagae strain CHC25 was further shown in an experimental microcosm set-up. In the light of this remarkable capacity, we propose to coin strain CHC25 Candidatus Porrumbacterium oxyphilus (class Holophagae, Phylum Acidobacteria), the first cultured representative with rhizosphere competence.
format	article
author	Ulisses Nunes da Rocha Caroline M Plugge Isabelle George Jan Dirk van Elsas Leonard Simon van Overbeek
author_facet	Ulisses Nunes da Rocha Caroline M Plugge Isabelle George Jan Dirk van Elsas Leonard Simon van Overbeek
author_sort	Ulisses Nunes da Rocha
title	The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
title_short	The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
title_full	The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
title_fullStr	The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
title_full_unstemmed	The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
title_sort	rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/43a196195d414822810abffca4374a96
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The rhizosphere selects for particular groups of acidobacteria and verrucomicrobia.

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