Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties

Abstract Optogenetics provides tools to control afferent activity in brain microcircuits. However, this requires optical methods that can evoke asynchronous and coordinated activity within neuronal ensembles in a spatio-temporally precise way. Here we describe a light patterning method, which combin...

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Autores principales:	Oscar Hernandez, Katarzyna Pietrajtis, Benjamin Mathieu, Stéphane Dieudonné
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Optogenetic Stimulation Brain Microcircuits Mossy Fiber (MF) MF Activity Gabazine Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43a2cff3fd934aada10f633d64dac0c6
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spelling	oai:doaj.org-article:43a2cff3fd934aada10f633d64dac0c62021-12-02T15:07:52ZOptogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties10.1038/s41598-018-32017-w2045-2322https://doaj.org/article/43a2cff3fd934aada10f633d64dac0c62018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32017-whttps://doaj.org/toc/2045-2322Abstract Optogenetics provides tools to control afferent activity in brain microcircuits. However, this requires optical methods that can evoke asynchronous and coordinated activity within neuronal ensembles in a spatio-temporally precise way. Here we describe a light patterning method, which combines MHz acousto-optic beam steering and adjustable low numerical aperture Gaussian beams, to achieve fast 2D targeting in scattering tissue. Using mossy fiber afferents to the cerebellar cortex as a testbed, we demonstrate single fiber optogenetic stimulation with micron-scale lateral resolution, >100 µm depth-penetration and 0.1 ms spiking precision. Protracted spatio-temporal patterns of light delivered by our illumination system evoked sustained asynchronous mossy fiber activity with excellent repeatability. Combining optical and electrical stimulations, we show that the cerebellar granular layer performs nonlinear integration, whereby sustained mossy fiber activity provides a permissive context for the transmission of salient inputs, enriching combinatorial views on mossy fiber pattern separation.Oscar HernandezKatarzyna PietrajtisBenjamin MathieuStéphane DieudonnéNature PortfolioarticleOptogenetic StimulationBrain MicrocircuitsMossy Fiber (MF)MF ActivityGabazineMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Optogenetic Stimulation Brain Microcircuits Mossy Fiber (MF) MF Activity Gabazine Medicine R Science Q
spellingShingle	Optogenetic Stimulation Brain Microcircuits Mossy Fiber (MF) MF Activity Gabazine Medicine R Science Q Oscar Hernandez Katarzyna Pietrajtis Benjamin Mathieu Stéphane Dieudonné Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
description	Abstract Optogenetics provides tools to control afferent activity in brain microcircuits. However, this requires optical methods that can evoke asynchronous and coordinated activity within neuronal ensembles in a spatio-temporally precise way. Here we describe a light patterning method, which combines MHz acousto-optic beam steering and adjustable low numerical aperture Gaussian beams, to achieve fast 2D targeting in scattering tissue. Using mossy fiber afferents to the cerebellar cortex as a testbed, we demonstrate single fiber optogenetic stimulation with micron-scale lateral resolution, >100 µm depth-penetration and 0.1 ms spiking precision. Protracted spatio-temporal patterns of light delivered by our illumination system evoked sustained asynchronous mossy fiber activity with excellent repeatability. Combining optical and electrical stimulations, we show that the cerebellar granular layer performs nonlinear integration, whereby sustained mossy fiber activity provides a permissive context for the transmission of salient inputs, enriching combinatorial views on mossy fiber pattern separation.
format	article
author	Oscar Hernandez Katarzyna Pietrajtis Benjamin Mathieu Stéphane Dieudonné
author_facet	Oscar Hernandez Katarzyna Pietrajtis Benjamin Mathieu Stéphane Dieudonné
author_sort	Oscar Hernandez
title	Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
title_short	Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
title_full	Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
title_fullStr	Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
title_full_unstemmed	Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
title_sort	optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/43a2cff3fd934aada10f633d64dac0c6
work_keys_str_mv	AT oscarhernandez optogeneticstimulationofcomplexspatiotemporalactivitypatternsbyacoustoopticlightsteeringprobescerebellargranularlayerintegrativeproperties AT katarzynapietrajtis optogeneticstimulationofcomplexspatiotemporalactivitypatternsbyacoustoopticlightsteeringprobescerebellargranularlayerintegrativeproperties AT benjaminmathieu optogeneticstimulationofcomplexspatiotemporalactivitypatternsbyacoustoopticlightsteeringprobescerebellargranularlayerintegrativeproperties AT stephanedieudonne optogeneticstimulationofcomplexspatiotemporalactivitypatternsbyacoustoopticlightsteeringprobescerebellargranularlayerintegrativeproperties
_version_	1718388380763422720

Optogenetic stimulation of complex spatio-temporal activity patterns by acousto-optic light steering probes cerebellar granular layer integrative properties

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