Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex

Noradrenaline is an important neuromodulator in the cerebellum. We previously found that noradrenaline depressed cerebellar Purkinje cell activity and climbing fiber–Purkinje cell synaptic transmission in vivo in mice. In this study, we investigated the effect of noradrenaline on the facial stimulat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bing-Xue Li, Hua Jin, Guang-Jian Zhang, Li-Na Cui, Chun-Ping Chu, De-Lai Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	adrenergic receptor (AR) cerebellum facial stimulation electrophysiology mossy fiber-granule cell synaptic transmission Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/43a4c0ffd372468eb5706e77ab5b6e1a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:43a4c0ffd372468eb5706e77ab5b6e1a
record_format	dspace
spelling	oai:doaj.org-article:43a4c0ffd372468eb5706e77ab5b6e1a2021-11-15T06:56:44ZEffect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex1662-453X10.3389/fnins.2021.785995https://doaj.org/article/43a4c0ffd372468eb5706e77ab5b6e1a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.785995/fullhttps://doaj.org/toc/1662-453XNoradrenaline is an important neuromodulator in the cerebellum. We previously found that noradrenaline depressed cerebellar Purkinje cell activity and climbing fiber–Purkinje cell synaptic transmission in vivo in mice. In this study, we investigated the effect of noradrenaline on the facial stimulation-evoked cerebellar cortical mossy fiber–granule cell synaptic transmission in urethane-anesthetized mice. In the presence of a γ-aminobutyrateA (GABAA) receptor antagonist, air-puff stimulation of the ipsilateral whisker pad evoked mossy fiber–granule cell synaptic transmission in the cerebellar granular layer, which expressed stimulus onset response, N1 and stimulus offset response, N2. Cerebellar surface perfusion of 25 μM noradrenaline induced decreases in the amplitude and area under the curve of N1 and N2, accompanied by an increase in the N2/N1 ratio. In the presence of a GABAA receptor blocker, noradrenaline induced a concentration-dependent decrease in the amplitude of N1, with a half-maximal inhibitory concentration of 25.45 μM. The noradrenaline-induced depression of the facial stimulation-evoked mossy fiber–granule cell synaptic transmission was reversed by additional application of an alpha-adrenergic receptor antagonist or an alpha-2 adrenergic receptor antagonist, but not by a beta-adrenergic receptor antagonist or an alpha-1 adrenergic receptor antagonist. Moreover, application of an alpha-2 adrenergic receptor agonist, UK14304, significantly decreased the synaptic response and prevented the noradrenaline-induced depression. Our results indicate that noradrenaline depresses facial stimulation-evoked mossy fiber–granule cell synaptic transmission via the alpha-2 adrenergic receptor in vivo in mice, suggesting that noradrenaline regulates sensory information integration and synaptic transmission in the cerebellar cortical granular layer.Bing-Xue LiBing-Xue LiHua JinHua JinGuang-Jian ZhangGuang-Jian ZhangLi-Na CuiLi-Na CuiChun-Ping ChuChun-Ping ChuDe-Lai QiuDe-Lai QiuFrontiers Media S.A.articleadrenergic receptor (AR)cerebellumfacial stimulationelectrophysiologymossy fiber-granule cell synaptic transmissionNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adrenergic receptor (AR) cerebellum facial stimulation electrophysiology mossy fiber-granule cell synaptic transmission Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	adrenergic receptor (AR) cerebellum facial stimulation electrophysiology mossy fiber-granule cell synaptic transmission Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Bing-Xue Li Bing-Xue Li Hua Jin Hua Jin Guang-Jian Zhang Guang-Jian Zhang Li-Na Cui Li-Na Cui Chun-Ping Chu Chun-Ping Chu De-Lai Qiu De-Lai Qiu Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex
description	Noradrenaline is an important neuromodulator in the cerebellum. We previously found that noradrenaline depressed cerebellar Purkinje cell activity and climbing fiber–Purkinje cell synaptic transmission in vivo in mice. In this study, we investigated the effect of noradrenaline on the facial stimulation-evoked cerebellar cortical mossy fiber–granule cell synaptic transmission in urethane-anesthetized mice. In the presence of a γ-aminobutyrateA (GABAA) receptor antagonist, air-puff stimulation of the ipsilateral whisker pad evoked mossy fiber–granule cell synaptic transmission in the cerebellar granular layer, which expressed stimulus onset response, N1 and stimulus offset response, N2. Cerebellar surface perfusion of 25 μM noradrenaline induced decreases in the amplitude and area under the curve of N1 and N2, accompanied by an increase in the N2/N1 ratio. In the presence of a GABAA receptor blocker, noradrenaline induced a concentration-dependent decrease in the amplitude of N1, with a half-maximal inhibitory concentration of 25.45 μM. The noradrenaline-induced depression of the facial stimulation-evoked mossy fiber–granule cell synaptic transmission was reversed by additional application of an alpha-adrenergic receptor antagonist or an alpha-2 adrenergic receptor antagonist, but not by a beta-adrenergic receptor antagonist or an alpha-1 adrenergic receptor antagonist. Moreover, application of an alpha-2 adrenergic receptor agonist, UK14304, significantly decreased the synaptic response and prevented the noradrenaline-induced depression. Our results indicate that noradrenaline depresses facial stimulation-evoked mossy fiber–granule cell synaptic transmission via the alpha-2 adrenergic receptor in vivo in mice, suggesting that noradrenaline regulates sensory information integration and synaptic transmission in the cerebellar cortical granular layer.
format	article
author	Bing-Xue Li Bing-Xue Li Hua Jin Hua Jin Guang-Jian Zhang Guang-Jian Zhang Li-Na Cui Li-Na Cui Chun-Ping Chu Chun-Ping Chu De-Lai Qiu De-Lai Qiu
author_facet	Bing-Xue Li Bing-Xue Li Hua Jin Hua Jin Guang-Jian Zhang Guang-Jian Zhang Li-Na Cui Li-Na Cui Chun-Ping Chu Chun-Ping Chu De-Lai Qiu De-Lai Qiu
author_sort	Bing-Xue Li
title	Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex
title_short	Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex
title_full	Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex
title_fullStr	Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex
title_full_unstemmed	Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex
title_sort	effect of noradrenaline on the facial stimulation-evoked mossy fiber-granule cell synaptic transmission in mouse cerebellar cortex
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/43a4c0ffd372468eb5706e77ab5b6e1a
work_keys_str_mv	AT bingxueli effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT bingxueli effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT huajin effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT huajin effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT guangjianzhang effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT guangjianzhang effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT linacui effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT linacui effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT chunpingchu effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT chunpingchu effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT delaiqiu effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex AT delaiqiu effectofnoradrenalineonthefacialstimulationevokedmossyfibergranulecellsynaptictransmissioninmousecerebellarcortex
_version_	1718428548305256448

Effect of Noradrenaline on the Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission in Mouse Cerebellar Cortex

Ejemplares similares