Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania

The European polecat (Mustela putorius Linnaeus, 1758) is in decline in Romania, often living near human settlements, from mountains to lowlands. They feed on a wide variety of small animals, including rodents, such as mice or rats. The occurrence of this parasite in polecats from Romania was mentio...

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Autores principales: Boros Z., Ionică A. M., Deak G., Mihalca A. D., Chișamera G., Constantinescu I. C., Adam C., Gherman C. M., Cozma V.
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Publicado: Sciendo 2021
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Acceso en línea:https://doaj.org/article/43a8edb1b1804ca9bb8e116265f0f89d
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spelling oai:doaj.org-article:43a8edb1b1804ca9bb8e116265f0f89d2021-12-05T14:11:07ZTrichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania1336-908310.2478/helm-2021-0032https://doaj.org/article/43a8edb1b1804ca9bb8e116265f0f89d2021-09-01T00:00:00Zhttps://doi.org/10.2478/helm-2021-0032https://doaj.org/toc/1336-9083The European polecat (Mustela putorius Linnaeus, 1758) is in decline in Romania, often living near human settlements, from mountains to lowlands. They feed on a wide variety of small animals, including rodents, such as mice or rats. The occurrence of this parasite in polecats from Romania was mentioned only once in 1991, but the parasite species was not confirmed by molecular biology. The study aimed to investigate the occurrence of Trichinella spp. in European polecats from Romania and to identify the parasite species by molecular tools. A total of 75 wild European polecats were examined by trichinoscopy and artificial digestion. A large number of animals were examined because of their wide distribution in Romanian territory and their presence near human settlements. For species determination, the positive muscle samples and the larvae recovered from artificial digestion were collected for DNA isolation and further processed by means of Multiplex PCR.Boros Z.Ionică A. M.Deak G.Mihalca A. D.Chișamera G.Constantinescu I. C.Adam C.Gherman C. M.Cozma V.Sciendoarticleeuropean polecatstrichinella spp.trichinoscopyartificial digestionmultiplex pcrAgriculture (General)S1-972Medicine (General)R5-920ENHelminthologia, Vol 58, Iss 3, Pp 323-327 (2021)
institution DOAJ
collection DOAJ
language EN
topic european polecats
trichinella spp.
trichinoscopy
artificial digestion
multiplex pcr
Agriculture (General)
S1-972
Medicine (General)
R5-920
spellingShingle european polecats
trichinella spp.
trichinoscopy
artificial digestion
multiplex pcr
Agriculture (General)
S1-972
Medicine (General)
R5-920
Boros Z.
Ionică A. M.
Deak G.
Mihalca A. D.
Chișamera G.
Constantinescu I. C.
Adam C.
Gherman C. M.
Cozma V.
Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania
description The European polecat (Mustela putorius Linnaeus, 1758) is in decline in Romania, often living near human settlements, from mountains to lowlands. They feed on a wide variety of small animals, including rodents, such as mice or rats. The occurrence of this parasite in polecats from Romania was mentioned only once in 1991, but the parasite species was not confirmed by molecular biology. The study aimed to investigate the occurrence of Trichinella spp. in European polecats from Romania and to identify the parasite species by molecular tools. A total of 75 wild European polecats were examined by trichinoscopy and artificial digestion. A large number of animals were examined because of their wide distribution in Romanian territory and their presence near human settlements. For species determination, the positive muscle samples and the larvae recovered from artificial digestion were collected for DNA isolation and further processed by means of Multiplex PCR.
format article
author Boros Z.
Ionică A. M.
Deak G.
Mihalca A. D.
Chișamera G.
Constantinescu I. C.
Adam C.
Gherman C. M.
Cozma V.
author_facet Boros Z.
Ionică A. M.
Deak G.
Mihalca A. D.
Chișamera G.
Constantinescu I. C.
Adam C.
Gherman C. M.
Cozma V.
author_sort Boros Z.
title Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania
title_short Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania
title_full Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania
title_fullStr Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania
title_full_unstemmed Trichinella spp. infection in European polecats (Mustela putorius Linnaeus, 1758) from Romania
title_sort trichinella spp. infection in european polecats (mustela putorius linnaeus, 1758) from romania
publisher Sciendo
publishDate 2021
url https://doaj.org/article/43a8edb1b1804ca9bb8e116265f0f89d
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