Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility

ABSTRACT Urgency urinary incontinence (UUI) and overactive bladder (OAB) can both potentially be influenced by commensal and urinary tract infection-associated bacteria. The sensing of bladder filling involves interplay between various components of the nervous system, eventually resulting in contra...

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Autores principales:	Behnam Abbasian, Aidan Shair, David B. O’Gorman, Ana M. Pena-Diaz, Liam Brennan, Kathleen Engelbrecht, David W. Koenig, Gregor Reid, Jeremy P. Burton
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	ATP Escherichia coli Gardnerella Lactobacillus extracellular Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/43af5899dd0b41d5b821452261871087
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spelling	oai:doaj.org-article:43af5899dd0b41d5b8214522618710872021-11-15T15:27:32ZPotential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility10.1128/mSphere.00439-192379-5042https://doaj.org/article/43af5899dd0b41d5b8214522618710872019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00439-19https://doaj.org/toc/2379-5042ABSTRACT Urgency urinary incontinence (UUI) and overactive bladder (OAB) can both potentially be influenced by commensal and urinary tract infection-associated bacteria. The sensing of bladder filling involves interplay between various components of the nervous system, eventually resulting in contraction of the detrusor muscle during micturition. This study models host responses to various urogenital bacteria, first by using urothelial bladder cell lines and then with myofibroblast contraction assays. To measure responses, we examined Ca2+ influx, gene expression, and alpha smooth muscle actin deposition assays. Organisms such as Escherichia coli and Gardnerella vaginalis were found to strongly induce Ca2+ influx and contraction, whereas Lactobacillus crispatus and L. gasseri did not induce this response. Additionally, supernatants from lactobacilli impeded Ca2+ influx and contraction induced by uropathogens. Upon further investigation of factors associated with purinergic signaling pathways, the Ca2+ influx and contraction of cells correlated with the amount of extracellular ATP produced by E. coli. Certain lactobacilli appear to mitigate this response by utilizing extracellular ATP or producing inhibitory compounds that may act as a receptor agonist or Ca2+ channel blocker. These findings suggest that members of the urinary microbiota may be influencing UUI or OAB. IMPORTANCE The ability of uropathogenic bacteria to release excitatory compounds, such as ATP, may act as a virulence factor to stimulate signaling pathways that could have profound effects on the urothelium, perhaps extending to the vagina. This may be countered by the ability of certain commensal urinary microbiota constituents, such as lactobacilli. Further understanding of these interactions is important for the treatment and prevention of UUI and OAB. The clinical implications may require a more targeted approach to enhance the commensal bacteria and reduce ATP release by pathogens.Behnam AbbasianAidan ShairDavid B. O’GormanAna M. Pena-DiazLiam BrennanKathleen EngelbrechtDavid W. KoenigGregor ReidJeremy P. BurtonAmerican Society for MicrobiologyarticleATPEscherichia coliGardnerellaLactobacillusextracellularMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ATP Escherichia coli Gardnerella Lactobacillus extracellular Microbiology QR1-502
spellingShingle	ATP Escherichia coli Gardnerella Lactobacillus extracellular Microbiology QR1-502 Behnam Abbasian Aidan Shair David B. O’Gorman Ana M. Pena-Diaz Liam Brennan Kathleen Engelbrecht David W. Koenig Gregor Reid Jeremy P. Burton Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility
description	ABSTRACT Urgency urinary incontinence (UUI) and overactive bladder (OAB) can both potentially be influenced by commensal and urinary tract infection-associated bacteria. The sensing of bladder filling involves interplay between various components of the nervous system, eventually resulting in contraction of the detrusor muscle during micturition. This study models host responses to various urogenital bacteria, first by using urothelial bladder cell lines and then with myofibroblast contraction assays. To measure responses, we examined Ca2+ influx, gene expression, and alpha smooth muscle actin deposition assays. Organisms such as Escherichia coli and Gardnerella vaginalis were found to strongly induce Ca2+ influx and contraction, whereas Lactobacillus crispatus and L. gasseri did not induce this response. Additionally, supernatants from lactobacilli impeded Ca2+ influx and contraction induced by uropathogens. Upon further investigation of factors associated with purinergic signaling pathways, the Ca2+ influx and contraction of cells correlated with the amount of extracellular ATP produced by E. coli. Certain lactobacilli appear to mitigate this response by utilizing extracellular ATP or producing inhibitory compounds that may act as a receptor agonist or Ca2+ channel blocker. These findings suggest that members of the urinary microbiota may be influencing UUI or OAB. IMPORTANCE The ability of uropathogenic bacteria to release excitatory compounds, such as ATP, may act as a virulence factor to stimulate signaling pathways that could have profound effects on the urothelium, perhaps extending to the vagina. This may be countered by the ability of certain commensal urinary microbiota constituents, such as lactobacilli. Further understanding of these interactions is important for the treatment and prevention of UUI and OAB. The clinical implications may require a more targeted approach to enhance the commensal bacteria and reduce ATP release by pathogens.
format	article
author	Behnam Abbasian Aidan Shair David B. O’Gorman Ana M. Pena-Diaz Liam Brennan Kathleen Engelbrecht David W. Koenig Gregor Reid Jeremy P. Burton
author_facet	Behnam Abbasian Aidan Shair David B. O’Gorman Ana M. Pena-Diaz Liam Brennan Kathleen Engelbrecht David W. Koenig Gregor Reid Jeremy P. Burton
author_sort	Behnam Abbasian
title	Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility
title_short	Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility
title_full	Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility
title_fullStr	Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility
title_full_unstemmed	Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility
title_sort	potential role of extracellular atp released by bacteria in bladder infection and contractility
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/43af5899dd0b41d5b821452261871087
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Potential Role of Extracellular ATP Released by Bacteria in Bladder Infection and Contractility

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