Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death

Abstract 6-OHDA plus ascorbic acid (AA) has long been used to induce Parkinson’s disease in rodents, while only 6-OHDA is commonly used to induce cell damage in cellular PD models. AA was believed to act as an anti-oxidant to prevent the degradation of 6-OHDA; however, some studies suggested that AA...

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Autores principales:	Sheng-Fang Wang, Liang-Feng Liu, Ming-Yue Wu, Cui-Zan Cai, Huanxing Su, Jieqiong Tan, Jia-Hong Lu, Min Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43af75dc2e204c9ca4ab9c1fd1ec99ca
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spelling	oai:doaj.org-article:43af75dc2e204c9ca4ab9c1fd1ec99ca2021-12-02T15:04:54ZBaicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death10.1038/s41598-017-07142-72045-2322https://doaj.org/article/43af75dc2e204c9ca4ab9c1fd1ec99ca2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07142-7https://doaj.org/toc/2045-2322Abstract 6-OHDA plus ascorbic acid (AA) has long been used to induce Parkinson’s disease in rodents, while only 6-OHDA is commonly used to induce cell damage in cellular PD models. AA was believed to act as an anti-oxidant to prevent the degradation of 6-OHDA; however, some studies suggested that AA dramatically enhanced the selectivity and toxicity of 6-OHDA. To understand the mechanisms by which 6-OHDA/AA induces cell death, we established a 6-OHDA/AA cell toxicity model in human dopaminergic neuroblastoma SH-SY5Y cells. We confirmed that the toxicity of 6-OHDA was dramatically increased in the presence of AA, and the toxicity can be prevented by a flavonoid, baicalein. Mechanistically, our research reveals that 6-OHDA/AA induces cell death mainly through the interruption of intracellular calcium homeostasis, which leads to calpain activation and mitochondrial damage. Baicalein prevents 6-OHDA/AA-induced intracellular calcium elevation as well as consequent mitochondria damage. Taken together, our study confirms that 6-OHDA/AA is a more sensitive model for inducing neuronal lesion in vitro and reveals the central role of intracellular calcium in 6-OHDA/AA-induced cell death. Our studies further show that baicalein prevents 6-OHDA/AA-induced cell death by inhibiting intracellular calcium elevation.Sheng-Fang WangLiang-Feng LiuMing-Yue WuCui-Zan CaiHuanxing SuJieqiong TanJia-Hong LuMin LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sheng-Fang Wang Liang-Feng Liu Ming-Yue Wu Cui-Zan Cai Huanxing Su Jieqiong Tan Jia-Hong Lu Min Li Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
description	Abstract 6-OHDA plus ascorbic acid (AA) has long been used to induce Parkinson’s disease in rodents, while only 6-OHDA is commonly used to induce cell damage in cellular PD models. AA was believed to act as an anti-oxidant to prevent the degradation of 6-OHDA; however, some studies suggested that AA dramatically enhanced the selectivity and toxicity of 6-OHDA. To understand the mechanisms by which 6-OHDA/AA induces cell death, we established a 6-OHDA/AA cell toxicity model in human dopaminergic neuroblastoma SH-SY5Y cells. We confirmed that the toxicity of 6-OHDA was dramatically increased in the presence of AA, and the toxicity can be prevented by a flavonoid, baicalein. Mechanistically, our research reveals that 6-OHDA/AA induces cell death mainly through the interruption of intracellular calcium homeostasis, which leads to calpain activation and mitochondrial damage. Baicalein prevents 6-OHDA/AA-induced intracellular calcium elevation as well as consequent mitochondria damage. Taken together, our study confirms that 6-OHDA/AA is a more sensitive model for inducing neuronal lesion in vitro and reveals the central role of intracellular calcium in 6-OHDA/AA-induced cell death. Our studies further show that baicalein prevents 6-OHDA/AA-induced cell death by inhibiting intracellular calcium elevation.
format	article
author	Sheng-Fang Wang Liang-Feng Liu Ming-Yue Wu Cui-Zan Cai Huanxing Su Jieqiong Tan Jia-Hong Lu Min Li
author_facet	Sheng-Fang Wang Liang-Feng Liu Ming-Yue Wu Cui-Zan Cai Huanxing Su Jieqiong Tan Jia-Hong Lu Min Li
author_sort	Sheng-Fang Wang
title	Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
title_short	Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
title_full	Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
title_fullStr	Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
title_full_unstemmed	Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
title_sort	baicalein prevents 6-ohda/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/43af75dc2e204c9ca4ab9c1fd1ec99ca
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Baicalein prevents 6-OHDA/ascorbic acid-induced calcium-dependent dopaminergic neuronal cell death

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