Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Abstract The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as ca...

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Autores principales:	Evgeny A. Shirshin, Yury I. Gurfinkel, Alexander V. Priezzhev, Victor V. Fadeev, Juergen Lademann, Maxim E. Darvin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43b3bb9674d241428d850a6a938a759e
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spelling	oai:doaj.org-article:43b3bb9674d241428d850a6a938a759e2021-12-02T15:06:03ZTwo-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization10.1038/s41598-017-01238-w2045-2322https://doaj.org/article/43b3bb9674d241428d850a6a938a759e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01238-whttps://doaj.org/toc/2045-2322Abstract The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.Evgeny A. ShirshinYury I. GurfinkelAlexander V. PriezzhevVictor V. FadeevJuergen LademannMaxim E. DarvinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evgeny A. Shirshin Yury I. Gurfinkel Alexander V. Priezzhev Victor V. Fadeev Juergen Lademann Maxim E. Darvin Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
description	Abstract The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.
format	article
author	Evgeny A. Shirshin Yury I. Gurfinkel Alexander V. Priezzhev Victor V. Fadeev Juergen Lademann Maxim E. Darvin
author_facet	Evgeny A. Shirshin Yury I. Gurfinkel Alexander V. Priezzhev Victor V. Fadeev Juergen Lademann Maxim E. Darvin
author_sort	Evgeny A. Shirshin
title	Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
title_short	Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
title_full	Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
title_fullStr	Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
title_full_unstemmed	Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
title_sort	two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/43b3bb9674d241428d850a6a938a759e
work_keys_str_mv	AT evgenyashirshin twophotonautofluorescencelifetimeimagingofhumanskinpapillarydermisinvivoassessmentofbloodcapillariesandstructuralproteinslocalization AT yuryigurfinkel twophotonautofluorescencelifetimeimagingofhumanskinpapillarydermisinvivoassessmentofbloodcapillariesandstructuralproteinslocalization AT alexandervpriezzhev twophotonautofluorescencelifetimeimagingofhumanskinpapillarydermisinvivoassessmentofbloodcapillariesandstructuralproteinslocalization AT victorvfadeev twophotonautofluorescencelifetimeimagingofhumanskinpapillarydermisinvivoassessmentofbloodcapillariesandstructuralproteinslocalization AT juergenlademann twophotonautofluorescencelifetimeimagingofhumanskinpapillarydermisinvivoassessmentofbloodcapillariesandstructuralproteinslocalization AT maximedarvin twophotonautofluorescencelifetimeimagingofhumanskinpapillarydermisinvivoassessmentofbloodcapillariesandstructuralproteinslocalization
_version_	1718388578402172928

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

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