Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells

Yi-Jang Lee1, Shih-Chieh Hung2&ndash;5, Mien-Sheng Chu41Department of Biomedical Imaging and Radiological Sciences, 2Institute of Clinical Medicine, 3Institute of Pharmacology, Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; 4Stem Cell Laboratory, Department of Medical Re...

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Autores principales:	Yi-Jang Lee, Shih-Chieh Hung, Mien-Sheng Chu
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/43ba6b047f7f4749a4d53ba7393b1bf0
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spelling	oai:doaj.org-article:43ba6b047f7f4749a4d53ba7393b1bf02021-12-02T01:33:37ZInvolvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells1178-6957https://doaj.org/article/43ba6b047f7f4749a4d53ba7393b1bf02010-11-01T00:00:00Zhttp://www.dovepress.com/involvement-of-notch1-inhibition-in-serum-stimulated-glia-and-oligoden-a5719https://doaj.org/toc/1178-6957Yi-Jang Lee1, Shih-Chieh Hung2&ndash;5, Mien-Sheng Chu41Department of Biomedical Imaging and Radiological Sciences, 2Institute of Clinical Medicine, 3Institute of Pharmacology, Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; 4Stem Cell Laboratory, Department of Medical Research and Education, 5Department of Orthopedics, Taipei Veterans General Hospital, Taipei 112, TaiwanAbstract: The use of in vitro oligodendrocyte differentiation for transplantation of stem cells to treat demyelinating diseases is an important consideration. In this study, we investigated the effects of serum on glia and oligodendrocyte differentiation from human mesenchymal stem cells (KP-hMSCs). We found that serum deprivation resulted in a reversible downregulation of glial- and oligodendrocyte-specific markers. Serum stimulated expression of oligodendrocyte markers, such as galactocerebroside, as well as Notch1 and JAK1 transcripts. Inhibition of Notch1 activation by the Notch inhibitor, MG132, led to enhanced expression of a serum-stimulated oligodendrocyte marker. This marker was undetectable in serum-deprived KP-hMSCs treated with MG132, suggesting that inhibition of Notch1 function is additive to serum-stimulated oligodendrocyte differentiation. Furthermore, a dominant-negative mutant RBP-J protein also inhibited Notch1 function and led to upregulation of oligodendrocyte-specific markers. Our results demonstrate that serum-stimulated oligodendrocyte differentiation is enhanced by the inhibition of Notch1-associated functions.Keywords: mesenchymal stem cells, glia and oligodendrocyte differentiation, Notch1 signaling, serum deprivation Yi-Jang LeeShih-Chieh HungMien-Sheng ChuDove Medical PressarticleCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol 2010, Iss default, Pp 165-173 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Yi-Jang Lee Shih-Chieh Hung Mien-Sheng Chu Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
description	Yi-Jang Lee1, Shih-Chieh Hung2&ndash;5, Mien-Sheng Chu41Department of Biomedical Imaging and Radiological Sciences, 2Institute of Clinical Medicine, 3Institute of Pharmacology, Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; 4Stem Cell Laboratory, Department of Medical Research and Education, 5Department of Orthopedics, Taipei Veterans General Hospital, Taipei 112, TaiwanAbstract: The use of in vitro oligodendrocyte differentiation for transplantation of stem cells to treat demyelinating diseases is an important consideration. In this study, we investigated the effects of serum on glia and oligodendrocyte differentiation from human mesenchymal stem cells (KP-hMSCs). We found that serum deprivation resulted in a reversible downregulation of glial- and oligodendrocyte-specific markers. Serum stimulated expression of oligodendrocyte markers, such as galactocerebroside, as well as Notch1 and JAK1 transcripts. Inhibition of Notch1 activation by the Notch inhibitor, MG132, led to enhanced expression of a serum-stimulated oligodendrocyte marker. This marker was undetectable in serum-deprived KP-hMSCs treated with MG132, suggesting that inhibition of Notch1 function is additive to serum-stimulated oligodendrocyte differentiation. Furthermore, a dominant-negative mutant RBP-J protein also inhibited Notch1 function and led to upregulation of oligodendrocyte-specific markers. Our results demonstrate that serum-stimulated oligodendrocyte differentiation is enhanced by the inhibition of Notch1-associated functions.Keywords: mesenchymal stem cells, glia and oligodendrocyte differentiation, Notch1 signaling, serum deprivation
format	article
author	Yi-Jang Lee Shih-Chieh Hung Mien-Sheng Chu
author_facet	Yi-Jang Lee Shih-Chieh Hung Mien-Sheng Chu
author_sort	Yi-Jang Lee
title	Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
title_short	Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
title_full	Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
title_fullStr	Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
title_full_unstemmed	Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
title_sort	involvement of notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/43ba6b047f7f4749a4d53ba7393b1bf0
work_keys_str_mv	AT yijanglee involvementofnotch1inhibitioninserumstimulatedgliaandoligodendrocytedifferentiationfromhumanmesenchymalstemcells AT shihchiehhung involvementofnotch1inhibitioninserumstimulatedgliaandoligodendrocytedifferentiationfromhumanmesenchymalstemcells AT mienshengchu involvementofnotch1inhibitioninserumstimulatedgliaandoligodendrocytedifferentiationfromhumanmesenchymalstemcells
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Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells

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