Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells

Abstract The detection of cancer biomarkers has recently attracted significant attention as a means of determining the correct course of treatment with targeted therapeutics. However, because the concentration of these biomarkers in blood is usually relatively low, highly sensitive biosensors for fl...

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Autores principales:	Mun-Ki Choi, Gil-Sung Kim, Jin-Tak Jeong, Jung-Taek Lim, Won-Yong Lee, Ahmad Umar, Sang-Kwon Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43bad948ee384e3eafadbb69d531def9
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spelling	oai:doaj.org-article:43bad948ee384e3eafadbb69d531def92021-12-02T11:40:58ZFunctionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells10.1038/s41598-017-14884-x2045-2322https://doaj.org/article/43bad948ee384e3eafadbb69d531def92017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14884-xhttps://doaj.org/toc/2045-2322Abstract The detection of cancer biomarkers has recently attracted significant attention as a means of determining the correct course of treatment with targeted therapeutics. However, because the concentration of these biomarkers in blood is usually relatively low, highly sensitive biosensors for fluorescence imaging and precise detection are needed. In this study, we have successfully developed vertical GaN micropillar (MP) based biosensors for fluorescence sensing and quantitative measurement of CA15-3 antigens. The highly ordered vertical GaN MP arrays result in the successful immobilization of CA15-3 antigens on each feature of the arrays, thereby allowing the detection of an individual fluorescence signal from the top surface of the arrays owing to the high regularity of fluorophore-tagged MP spots and relatively low background signal. Therefore, our fluorescence-labeled and CA15-3 functionalized vertical GaN-MP-based biosensor is suitable for the selective quantitative analysis of secreted CA15-3 antigens from MCF-7 cell lines, and helps in the early diagnosis and prognosis of serious diseases as well as the monitoring of the therapeutic response of breast cancer patients.Mun-Ki ChoiGil-Sung KimJin-Tak JeongJung-Taek LimWon-Yong LeeAhmad UmarSang-Kwon LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mun-Ki Choi Gil-Sung Kim Jin-Tak Jeong Jung-Taek Lim Won-Yong Lee Ahmad Umar Sang-Kwon Lee Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
description	Abstract The detection of cancer biomarkers has recently attracted significant attention as a means of determining the correct course of treatment with targeted therapeutics. However, because the concentration of these biomarkers in blood is usually relatively low, highly sensitive biosensors for fluorescence imaging and precise detection are needed. In this study, we have successfully developed vertical GaN micropillar (MP) based biosensors for fluorescence sensing and quantitative measurement of CA15-3 antigens. The highly ordered vertical GaN MP arrays result in the successful immobilization of CA15-3 antigens on each feature of the arrays, thereby allowing the detection of an individual fluorescence signal from the top surface of the arrays owing to the high regularity of fluorophore-tagged MP spots and relatively low background signal. Therefore, our fluorescence-labeled and CA15-3 functionalized vertical GaN-MP-based biosensor is suitable for the selective quantitative analysis of secreted CA15-3 antigens from MCF-7 cell lines, and helps in the early diagnosis and prognosis of serious diseases as well as the monitoring of the therapeutic response of breast cancer patients.
format	article
author	Mun-Ki Choi Gil-Sung Kim Jin-Tak Jeong Jung-Taek Lim Won-Yong Lee Ahmad Umar Sang-Kwon Lee
author_facet	Mun-Ki Choi Gil-Sung Kim Jin-Tak Jeong Jung-Taek Lim Won-Yong Lee Ahmad Umar Sang-Kwon Lee
author_sort	Mun-Ki Choi
title	Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
title_short	Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
title_full	Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
title_fullStr	Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
title_full_unstemmed	Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
title_sort	functionalized vertical gan micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/43bad948ee384e3eafadbb69d531def9
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Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells

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