Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases

Emerging antibiotic resistance has become a cosmopolitan problem and evoking researchers to search for new antimicrobials from natural constituents. The present study was intended to test the antimicrobial potential of traditionally used unexplored polyherbal recipes for curing digestive ailments. A...

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Autores principales:	Sakina Mussarat, Muhammad Adnan, Shaheen Begum, Shafiq Ur Rehman, Abeer Hashem, Elsayed Fathi Abd_Allah
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Polyherbal Gastrointestinal infections In-vitro Antibiotic resistance Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/43baf0f4613f4d7c8a7802165a9976e2
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spelling	oai:doaj.org-article:43baf0f4613f4d7c8a7802165a9976e22021-11-20T04:56:25ZAntimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases1319-562X10.1016/j.sjbs.2021.07.053https://doaj.org/article/43baf0f4613f4d7c8a7802165a9976e22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006367https://doaj.org/toc/1319-562XEmerging antibiotic resistance has become a cosmopolitan problem and evoking researchers to search for new antimicrobials from natural constituents. The present study was intended to test the antimicrobial potential of traditionally used unexplored polyherbal recipes for curing digestive ailments. A total of 25 plants species were combined in different ratios to form 14 polyherbal recipes. After collecting and grinding plant parts, methanolic extracts of 14 polyherbal recipes were prepared by the cold maceration process. Antibacterial and antifungal activity of the polyherbal extracts was checked by agar well diffusion method at a concentration of 50 mg/ ml while minimum inhibitory concentration (MIC) was determined by serial dilution method. Polyherbal recipes B and D showed significant inhibition zone each against Vibrio cholerae (25.63; p < 0.001). Recipe G (23.33; p < 0.001) showed better efficacy against Escherichia coli. Recipe E and G significantly inhibited Proteus species (28.33; 24.33; p < 0.001). Recipe B was highly effective against Salmonella typhi. Recipe C, A and F had significant antifungal affect and inhibited Aspergillus nigar (28.67; p < 0.05), Aspergillus fumigatus (27; p < 0.01) Trichoderma (30; p < 0.001), Rhizopus (19.67; p < 0.01), and Fusarium graminearum (28.67;p < 0.001). Polyherbal formulations A, B, D, K, and N were active with the lowest concentration. MIC ranges within 3.12–25 mg/ml while minimum bactericidal concentration (MBC) between 12.5 and 50 mg/ml. Polyherbal recipes’ A, B, D, G, K and N have enhanced antimicrobial potential with better efficacy than tested antibiotics and should be evaluated for further scientific validation.Sakina MussaratMuhammad AdnanShaheen BegumShafiq Ur RehmanAbeer HashemElsayed Fathi Abd_AllahElsevierarticlePolyherbalGastrointestinal infectionsIn-vitroAntibiotic resistanceBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6829-6843 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polyherbal Gastrointestinal infections In-vitro Antibiotic resistance Biology (General) QH301-705.5
spellingShingle	Polyherbal Gastrointestinal infections In-vitro Antibiotic resistance Biology (General) QH301-705.5 Sakina Mussarat Muhammad Adnan Shaheen Begum Shafiq Ur Rehman Abeer Hashem Elsayed Fathi Abd_Allah Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
description	Emerging antibiotic resistance has become a cosmopolitan problem and evoking researchers to search for new antimicrobials from natural constituents. The present study was intended to test the antimicrobial potential of traditionally used unexplored polyherbal recipes for curing digestive ailments. A total of 25 plants species were combined in different ratios to form 14 polyherbal recipes. After collecting and grinding plant parts, methanolic extracts of 14 polyherbal recipes were prepared by the cold maceration process. Antibacterial and antifungal activity of the polyherbal extracts was checked by agar well diffusion method at a concentration of 50 mg/ ml while minimum inhibitory concentration (MIC) was determined by serial dilution method. Polyherbal recipes B and D showed significant inhibition zone each against Vibrio cholerae (25.63; p < 0.001). Recipe G (23.33; p < 0.001) showed better efficacy against Escherichia coli. Recipe E and G significantly inhibited Proteus species (28.33; 24.33; p < 0.001). Recipe B was highly effective against Salmonella typhi. Recipe C, A and F had significant antifungal affect and inhibited Aspergillus nigar (28.67; p < 0.05), Aspergillus fumigatus (27; p < 0.01) Trichoderma (30; p < 0.001), Rhizopus (19.67; p < 0.01), and Fusarium graminearum (28.67;p < 0.001). Polyherbal formulations A, B, D, K, and N were active with the lowest concentration. MIC ranges within 3.12–25 mg/ml while minimum bactericidal concentration (MBC) between 12.5 and 50 mg/ml. Polyherbal recipes’ A, B, D, G, K and N have enhanced antimicrobial potential with better efficacy than tested antibiotics and should be evaluated for further scientific validation.
format	article
author	Sakina Mussarat Muhammad Adnan Shaheen Begum Shafiq Ur Rehman Abeer Hashem Elsayed Fathi Abd_Allah
author_facet	Sakina Mussarat Muhammad Adnan Shaheen Begum Shafiq Ur Rehman Abeer Hashem Elsayed Fathi Abd_Allah
author_sort	Sakina Mussarat
title	Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
title_short	Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
title_full	Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
title_fullStr	Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
title_full_unstemmed	Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
title_sort	antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/43baf0f4613f4d7c8a7802165a9976e2
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Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases

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