Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study

Background: Leprosy is a chronic infectious disease where delay in diagnosis or treatment can lead to deformities and disabilities for the rest of the patient’s life. Definite diagnosis of leprosy has long been based on clinical picture, histopathology and/or the detection of acid-fast bacilli (AFB)...

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Autores principales:	Nehal Mostafa kamel, Osama Hashem, Hassan Khodair, Hesham Abd El-Samee
Formato:	article
Lenguaje:	EN
Publicado:	Al-Azhar University, Faculty of Medicine (Damietta) 2020
Materias:	leprosy polymerase chain reaction slit skin smear mycobacterium leprae acid-fast bacilli Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/43bf84eb3be845a3ace45a10235db84c
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spelling	oai:doaj.org-article:43bf84eb3be845a3ace45a10235db84c2021-12-02T14:02:00ZPolymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study2636-41742682-378010.21608/ijma.2020.30181.1126https://doaj.org/article/43bf84eb3be845a3ace45a10235db84c2020-07-01T00:00:00Zhttps://ijma.journals.ekb.eg/article_95155_df7d2591321b063515d1b1a18de449c3.pdfhttps://doaj.org/toc/2636-4174https://doaj.org/toc/2682-3780Background: Leprosy is a chronic infectious disease where delay in diagnosis or treatment can lead to deformities and disabilities for the rest of the patient’s life. Definite diagnosis of leprosy has long been based on clinical picture, histopathology and/or the detection of acid-fast bacilli (AFB) from tissue smears or tissue sections stained by Ziehl-Neelsen. Aim of the work: We evaluatedin this study, the usefulness of PCR for detection of M.leprae in the serum, as less invasive technique, in comparison to skin slit smear for the diagnosis of leprosy. Patients and Methods:This study included 30 non-treated patients of leprosy (9 multibacillary, 21 paucibacillary) skin slit smears had been taken. In addition, serum samples had been collected for polymerase chain reaction [PCR] test. Results: Negative Slit Skin Smear for AFB had been obtained in 20 cases with a rate of positivity of 33%. Serum PCR was positive in 20 patients giving an overall rate of positivity of 67% which confirm the diagnosis in 11 out of 20 cases with slit skin smear [SSS] negative. Conclusion: PCR does show to be more sensitive than slit skin smear [SSS] indicating its future use for diagnostic purposes especially in early leprosy cases.Nehal Mostafa kamelOsama HashemHassan KhodairHesham Abd El-SameeAl-Azhar University, Faculty of Medicine (Damietta)articleleprosypolymerase chain reactionslit skin smearmycobacterium lepraeacid-fast bacilliMedicine (General)R5-920ENInternational Journal of Medical Arts, Vol 2, Iss 3, Pp 650-654 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	leprosy polymerase chain reaction slit skin smear mycobacterium leprae acid-fast bacilli Medicine (General) R5-920
spellingShingle	leprosy polymerase chain reaction slit skin smear mycobacterium leprae acid-fast bacilli Medicine (General) R5-920 Nehal Mostafa kamel Osama Hashem Hassan Khodair Hesham Abd El-Samee Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study
description	Background: Leprosy is a chronic infectious disease where delay in diagnosis or treatment can lead to deformities and disabilities for the rest of the patient’s life. Definite diagnosis of leprosy has long been based on clinical picture, histopathology and/or the detection of acid-fast bacilli (AFB) from tissue smears or tissue sections stained by Ziehl-Neelsen. Aim of the work: We evaluatedin this study, the usefulness of PCR for detection of M.leprae in the serum, as less invasive technique, in comparison to skin slit smear for the diagnosis of leprosy. Patients and Methods:This study included 30 non-treated patients of leprosy (9 multibacillary, 21 paucibacillary) skin slit smears had been taken. In addition, serum samples had been collected for polymerase chain reaction [PCR] test. Results: Negative Slit Skin Smear for AFB had been obtained in 20 cases with a rate of positivity of 33%. Serum PCR was positive in 20 patients giving an overall rate of positivity of 67% which confirm the diagnosis in 11 out of 20 cases with slit skin smear [SSS] negative. Conclusion: PCR does show to be more sensitive than slit skin smear [SSS] indicating its future use for diagnostic purposes especially in early leprosy cases.
format	article
author	Nehal Mostafa kamel Osama Hashem Hassan Khodair Hesham Abd El-Samee
author_facet	Nehal Mostafa kamel Osama Hashem Hassan Khodair Hesham Abd El-Samee
author_sort	Nehal Mostafa kamel
title	Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study
title_short	Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study
title_full	Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study
title_fullStr	Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study
title_full_unstemmed	Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study
title_sort	polymerase chain reaction versus slit skin smear in diagnosis of leprosy; a cross sectional study
publisher	Al-Azhar University, Faculty of Medicine (Damietta)
publishDate	2020
url	https://doaj.org/article/43bf84eb3be845a3ace45a10235db84c
work_keys_str_mv	AT nehalmostafakamel polymerasechainreactionversusslitskinsmearindiagnosisofleprosyacrosssectionalstudy AT osamahashem polymerasechainreactionversusslitskinsmearindiagnosisofleprosyacrosssectionalstudy AT hassankhodair polymerasechainreactionversusslitskinsmearindiagnosisofleprosyacrosssectionalstudy AT heshamabdelsamee polymerasechainreactionversusslitskinsmearindiagnosisofleprosyacrosssectionalstudy
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Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study

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