Establishment of a rotavirus-infected zebrafish model and its application in drug screening

Rotavirus (RV) is one of the main pathogens that induce infantile diarrhea and by now no effective drugs are available for RV-induced infantile diarrhea. Thus the development of novel models is of vital importance for the pathological research of RV-induced infantile diarrhea, as well as the progres...

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Autores principales:	Lijun Song, Xuemei Zhu, Yujing Zhou, Yuxuan Feng, Guiqin Dai, Jiabo Chen, Yang Chen, Feng Li, Wenchang Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/43c2331e55c84e3d83ee4cd88b197ee6
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spelling	oai:doaj.org-article:43c2331e55c84e3d83ee4cd88b197ee62021-11-14T04:30:28ZEstablishment of a rotavirus-infected zebrafish model and its application in drug screening0753-332210.1016/j.biopha.2021.112398https://doaj.org/article/43c2331e55c84e3d83ee4cd88b197ee62022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011847https://doaj.org/toc/0753-3322Rotavirus (RV) is one of the main pathogens that induce infantile diarrhea and by now no effective drugs are available for RV-induced infantile diarrhea. Thus the development of novel models is of vital importance for the pathological research of RV-induced infantile diarrhea, as well as the progress of the associated treatment strategy. Here we introduced for the first time that RV-Wa strain and RV-SA-11 strain could infect 5 dpf(day post fertilization) and 28 dpf larvae, to induce infantile diarrhea model that was highly consistent with the clinical infection of infants. RV infection significantly changed the signs, survival rate and inflammation of larvae. Some important indicators, including the levels of RV antigen VP4 and VP6, the in vivo RV tracking, and the RV particles were also analyzed, which collectively demonstrated that the model was successfully established. More importantly, we also determined the potentials of the proposed RV-infected zebrafish model for anti-viral drug assessment. In conclusion, we established a RV-infected zebrafish model with formulated relevant indicators both larvae and adult fish, which might be served as a high throughput platform for antiviral drug screening.Lijun SongXuemei ZhuYujing ZhouYuxuan FengGuiqin DaiJiabo ChenYang ChenFeng LiWenchang ZhaoElsevierarticleTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112398- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 Lijun Song Xuemei Zhu Yujing Zhou Yuxuan Feng Guiqin Dai Jiabo Chen Yang Chen Feng Li Wenchang Zhao Establishment of a rotavirus-infected zebrafish model and its application in drug screening
description	Rotavirus (RV) is one of the main pathogens that induce infantile diarrhea and by now no effective drugs are available for RV-induced infantile diarrhea. Thus the development of novel models is of vital importance for the pathological research of RV-induced infantile diarrhea, as well as the progress of the associated treatment strategy. Here we introduced for the first time that RV-Wa strain and RV-SA-11 strain could infect 5 dpf(day post fertilization) and 28 dpf larvae, to induce infantile diarrhea model that was highly consistent with the clinical infection of infants. RV infection significantly changed the signs, survival rate and inflammation of larvae. Some important indicators, including the levels of RV antigen VP4 and VP6, the in vivo RV tracking, and the RV particles were also analyzed, which collectively demonstrated that the model was successfully established. More importantly, we also determined the potentials of the proposed RV-infected zebrafish model for anti-viral drug assessment. In conclusion, we established a RV-infected zebrafish model with formulated relevant indicators both larvae and adult fish, which might be served as a high throughput platform for antiviral drug screening.
format	article
author	Lijun Song Xuemei Zhu Yujing Zhou Yuxuan Feng Guiqin Dai Jiabo Chen Yang Chen Feng Li Wenchang Zhao
author_facet	Lijun Song Xuemei Zhu Yujing Zhou Yuxuan Feng Guiqin Dai Jiabo Chen Yang Chen Feng Li Wenchang Zhao
author_sort	Lijun Song
title	Establishment of a rotavirus-infected zebrafish model and its application in drug screening
title_short	Establishment of a rotavirus-infected zebrafish model and its application in drug screening
title_full	Establishment of a rotavirus-infected zebrafish model and its application in drug screening
title_fullStr	Establishment of a rotavirus-infected zebrafish model and its application in drug screening
title_full_unstemmed	Establishment of a rotavirus-infected zebrafish model and its application in drug screening
title_sort	establishment of a rotavirus-infected zebrafish model and its application in drug screening
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/43c2331e55c84e3d83ee4cd88b197ee6
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Establishment of a rotavirus-infected zebrafish model and its application in drug screening

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