Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation

Silica dioxide nanoparticles (SiONPs) have been increasingly used in various industries; however, this has raised concerns regarding their potential toxicity. SiONPs are also a major component in the Asian sand dust that causes pulmonary diseases among the general public. Melatonin exerts some inhib...

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Autores principales:	Je-Oh Lim, Se-Jin Lee, Woong-Il Kim, So-Won Pak, Jong-Choon Kim, Joong-Sun Kim, Young-Kwon Cho, In-Chul Lee, In-Sik Shin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	melatonin silica dioxide nanoparticle lung inflammation human airway epithelial cell line thioredoxin-interacting protein Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/43c4868c58bb46619bd59f9d152b7ca5
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spelling	oai:doaj.org-article:43c4868c58bb46619bd59f9d152b7ca52021-11-25T16:27:59ZMelatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation10.3390/antiox101117652076-3921https://doaj.org/article/43c4868c58bb46619bd59f9d152b7ca52021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1765https://doaj.org/toc/2076-3921Silica dioxide nanoparticles (SiONPs) have been increasingly used in various industries; however, this has raised concerns regarding their potential toxicity. SiONPs are also a major component in the Asian sand dust that causes pulmonary diseases among the general public. Melatonin exerts some inhibitory effects against lung inflammation. In this study, we explored the therapeutic properties of melatonin against lung inflammation using an SiONPs-induced lung inflammation murine model and SiONPs-stimulated H292 cells, human airway epithelial cell line, by focusing on the involvement of thioredoxin-interacting protein (TXNIP) in the modulation of the MAPKs/AP-1 axis. We induced an inflammatory response by exposing mouse lungs and the H292 cells to SiONPs and confirmed the anti-inflammatory effect of melatonin. Melatonin inhibited the expression of various inflammatory mediators, including <i>TNF-α</i>, <i>IL-6</i>, and <i>IL-1β</i>, in SiONPs-exposed mice and SiONPs-stimulated H292 cells; this inhibition contributed to a decline in inflammatory cell accumulation in the lung tissues. Furthermore, melatonin treatment decreased the expression of MAPKs and AP-1 by downregulating TXNIP, eventually decreasing the production of SiONPs-induced inflammatory mediators. Overall, these data suggest that melatonin reduces SiONPs-induced lung inflammation by downregulating the TXNIP/MAPKs/AP-1 signalling pathway, thereby supporting the use of melatonin as an effective approach to control SiONPs-induced lung inflammation.Je-Oh LimSe-Jin LeeWoong-Il KimSo-Won PakJong-Choon KimJoong-Sun KimYoung-Kwon ChoIn-Chul LeeIn-Sik ShinMDPI AGarticlemelatoninsilica dioxide nanoparticlelung inflammationhuman airway epithelial cell linethioredoxin-interacting proteinTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1765, p 1765 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melatonin silica dioxide nanoparticle lung inflammation human airway epithelial cell line thioredoxin-interacting protein Therapeutics. Pharmacology RM1-950
spellingShingle	melatonin silica dioxide nanoparticle lung inflammation human airway epithelial cell line thioredoxin-interacting protein Therapeutics. Pharmacology RM1-950 Je-Oh Lim Se-Jin Lee Woong-Il Kim So-Won Pak Jong-Choon Kim Joong-Sun Kim Young-Kwon Cho In-Chul Lee In-Sik Shin Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
description	Silica dioxide nanoparticles (SiONPs) have been increasingly used in various industries; however, this has raised concerns regarding their potential toxicity. SiONPs are also a major component in the Asian sand dust that causes pulmonary diseases among the general public. Melatonin exerts some inhibitory effects against lung inflammation. In this study, we explored the therapeutic properties of melatonin against lung inflammation using an SiONPs-induced lung inflammation murine model and SiONPs-stimulated H292 cells, human airway epithelial cell line, by focusing on the involvement of thioredoxin-interacting protein (TXNIP) in the modulation of the MAPKs/AP-1 axis. We induced an inflammatory response by exposing mouse lungs and the H292 cells to SiONPs and confirmed the anti-inflammatory effect of melatonin. Melatonin inhibited the expression of various inflammatory mediators, including <i>TNF-α</i>, <i>IL-6</i>, and <i>IL-1β</i>, in SiONPs-exposed mice and SiONPs-stimulated H292 cells; this inhibition contributed to a decline in inflammatory cell accumulation in the lung tissues. Furthermore, melatonin treatment decreased the expression of MAPKs and AP-1 by downregulating TXNIP, eventually decreasing the production of SiONPs-induced inflammatory mediators. Overall, these data suggest that melatonin reduces SiONPs-induced lung inflammation by downregulating the TXNIP/MAPKs/AP-1 signalling pathway, thereby supporting the use of melatonin as an effective approach to control SiONPs-induced lung inflammation.
format	article
author	Je-Oh Lim Se-Jin Lee Woong-Il Kim So-Won Pak Jong-Choon Kim Joong-Sun Kim Young-Kwon Cho In-Chul Lee In-Sik Shin
author_facet	Je-Oh Lim Se-Jin Lee Woong-Il Kim So-Won Pak Jong-Choon Kim Joong-Sun Kim Young-Kwon Cho In-Chul Lee In-Sik Shin
author_sort	Je-Oh Lim
title	Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_short	Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_full	Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_fullStr	Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_full_unstemmed	Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_sort	melatonin alleviates silica nanoparticle-induced lung inflammation via thioredoxin-interacting protein downregulation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/43c4868c58bb46619bd59f9d152b7ca5
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Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation

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