A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

Abstract Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measur...

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Autores principales: Juhyun Lee, Tzu-Chieh Chou, Dongyang Kang, Hanul Kang, Junjie Chen, Kyung In Baek, Wei Wang, Yichen Ding, Dino Di Carlo, Yu-Chong Tai, Tzung K. Hsiai
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Science
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Acceso en línea:https://doaj.org/article/43c6bbf9166f437aa0260183151a7e83
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spelling oai:doaj.org-article:43c6bbf9166f437aa0260183151a7e832021-12-02T11:52:57ZA Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates10.1038/s41598-017-02253-72045-2322https://doaj.org/article/43c6bbf9166f437aa0260183151a7e832017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02253-7https://doaj.org/toc/2045-2322Abstract Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s−1), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s−1 were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.Juhyun LeeTzu-Chieh ChouDongyang KangHanul KangJunjie ChenKyung In BaekWei WangYichen DingDino Di CarloYu-Chong TaiTzung K. HsiaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Juhyun Lee
Tzu-Chieh Chou
Dongyang Kang
Hanul Kang
Junjie Chen
Kyung In Baek
Wei Wang
Yichen Ding
Dino Di Carlo
Yu-Chong Tai
Tzung K. Hsiai
A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
description Abstract Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s−1), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s−1 were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.
format article
author Juhyun Lee
Tzu-Chieh Chou
Dongyang Kang
Hanul Kang
Junjie Chen
Kyung In Baek
Wei Wang
Yichen Ding
Dino Di Carlo
Yu-Chong Tai
Tzung K. Hsiai
author_facet Juhyun Lee
Tzu-Chieh Chou
Dongyang Kang
Hanul Kang
Junjie Chen
Kyung In Baek
Wei Wang
Yichen Ding
Dino Di Carlo
Yu-Chong Tai
Tzung K. Hsiai
author_sort Juhyun Lee
title A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
title_short A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
title_full A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
title_fullStr A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
title_full_unstemmed A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates
title_sort rapid capillary-pressure driven micro-channel to demonstrate newtonian fluid behavior of zebrafish blood at high shear rates
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/43c6bbf9166f437aa0260183151a7e83
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