Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway

Taro (Colocasia esculenta) is an important tuber crop and staple food. Taro corms have higher nutritional value and starch contents as compared to most of the other root/tuber crops. However, the growth and development of the taro rhizome have not been critically examined in terms of transcriptomic...

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Autores principales:	Weiqing Dong, Fanglian He, Huiping Jiang, Lili Liu, Zuyang Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	carbohydrates gene expression small RNA corm development taro (Colocasia esculenta L. Schott) Genetics QH426-470
Acceso en línea:	https://doaj.org/article/43c79725b61741dc8533f434f0cb5e20
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spelling	oai:doaj.org-article:43c79725b61741dc8533f434f0cb5e202021-11-08T14:53:07ZComparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway1664-802110.3389/fgene.2021.771081https://doaj.org/article/43c79725b61741dc8533f434f0cb5e202021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.771081/fullhttps://doaj.org/toc/1664-8021Taro (Colocasia esculenta) is an important tuber crop and staple food. Taro corms have higher nutritional value and starch contents as compared to most of the other root/tuber crops. However, the growth and development of the taro rhizome have not been critically examined in terms of transcriptomic signatures in general or specific to carbohydrates (starch and sucrose) accumulation. In current study, we have conducted a comprehensive survey of transcripts in taro corms aged 1, 2, 3, 4, 5, and 8 months. In this context, we have employed a whole transcriptome sequencing approach for identification of mRNAs, CircRNAs, and miRNAs in corms and performed functional enrichment analysis of the screened differentially expressed RNAs. A total of 11,203 mRNAs, 245 CircRNAs, and 299 miRNAs were obtained from six developmental stages. The mRNAs included 139 DEGs associated with 24 important enzymes of starch and sucrose metabolism. The expression of genes encoding key enzymes of starch and sucrose metabolism pathway (GBSS, AGPase, UGPase, SP, SSS, βFRUCT and SuSy) demonstrated significant variations at the stage of 4 months (S4). A total of 191 CircRNAs were differentially expressed between the studied comparisons of growth stages and 99 of these were associated with those miRNA (or target genes) that were enriched in starch and sucrose metabolism pathway. We also identified 205 miRNAs including 46 miRNAs targeting DEGs enriched in starch and sucrose biosynthesis pathway. The results of current study provide valuable resources for future exploration of the molecular mechanisms involved in the starch properties of Taro.Weiqing DongFanglian HeHuiping JiangLili LiuZuyang QiuFrontiers Media S.A.articlecarbohydratesgene expressionsmall RNAcorm developmenttaro (Colocasia esculenta L. Schott)GeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbohydrates gene expression small RNA corm development taro (Colocasia esculenta L. Schott) Genetics QH426-470
spellingShingle	carbohydrates gene expression small RNA corm development taro (Colocasia esculenta L. Schott) Genetics QH426-470 Weiqing Dong Fanglian He Huiping Jiang Lili Liu Zuyang Qiu Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway
description	Taro (Colocasia esculenta) is an important tuber crop and staple food. Taro corms have higher nutritional value and starch contents as compared to most of the other root/tuber crops. However, the growth and development of the taro rhizome have not been critically examined in terms of transcriptomic signatures in general or specific to carbohydrates (starch and sucrose) accumulation. In current study, we have conducted a comprehensive survey of transcripts in taro corms aged 1, 2, 3, 4, 5, and 8 months. In this context, we have employed a whole transcriptome sequencing approach for identification of mRNAs, CircRNAs, and miRNAs in corms and performed functional enrichment analysis of the screened differentially expressed RNAs. A total of 11,203 mRNAs, 245 CircRNAs, and 299 miRNAs were obtained from six developmental stages. The mRNAs included 139 DEGs associated with 24 important enzymes of starch and sucrose metabolism. The expression of genes encoding key enzymes of starch and sucrose metabolism pathway (GBSS, AGPase, UGPase, SP, SSS, βFRUCT and SuSy) demonstrated significant variations at the stage of 4 months (S4). A total of 191 CircRNAs were differentially expressed between the studied comparisons of growth stages and 99 of these were associated with those miRNA (or target genes) that were enriched in starch and sucrose metabolism pathway. We also identified 205 miRNAs including 46 miRNAs targeting DEGs enriched in starch and sucrose biosynthesis pathway. The results of current study provide valuable resources for future exploration of the molecular mechanisms involved in the starch properties of Taro.
format	article
author	Weiqing Dong Fanglian He Huiping Jiang Lili Liu Zuyang Qiu
author_facet	Weiqing Dong Fanglian He Huiping Jiang Lili Liu Zuyang Qiu
author_sort	Weiqing Dong
title	Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway
title_short	Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway
title_full	Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway
title_fullStr	Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway
title_full_unstemmed	Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway
title_sort	comparative transcriptome sequencing of taro corm development with a focus on the starch and sucrose metabolism pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/43c79725b61741dc8533f434f0cb5e20
work_keys_str_mv	AT weiqingdong comparativetranscriptomesequencingoftarocormdevelopmentwithafocusonthestarchandsucrosemetabolismpathway AT fanglianhe comparativetranscriptomesequencingoftarocormdevelopmentwithafocusonthestarchandsucrosemetabolismpathway AT huipingjiang comparativetranscriptomesequencingoftarocormdevelopmentwithafocusonthestarchandsucrosemetabolismpathway AT lililiu comparativetranscriptomesequencingoftarocormdevelopmentwithafocusonthestarchandsucrosemetabolismpathway AT zuyangqiu comparativetranscriptomesequencingoftarocormdevelopmentwithafocusonthestarchandsucrosemetabolismpathway
_version_	1718442125793689600

Comparative Transcriptome Sequencing of Taro Corm Development With a Focus on the Starch and Sucrose Metabolism Pathway

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