Topology and organization of the Salmonella typhimurium type III secretion needle complex components.

The correct organization of single subunits of multi-protein machines in a three dimensional context is critical for their functionality. Type III secretion systems (T3SS) are molecular machines with the capacity to deliver bacterial effector proteins into host cells and are fundamental for the biol...

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Autores principales:	Oliver Schraidt, Matthew D Lefebre, Matthias J Brunner, Wolfgang H Schmied, Andreas Schmidt, Julia Radics, Karl Mechtler, Jorge E Galán, Thomas C Marlovits
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/43c867db15814e329b64201206fda016
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spelling	oai:doaj.org-article:43c867db15814e329b64201206fda0162021-11-25T05:48:11ZTopology and organization of the Salmonella typhimurium type III secretion needle complex components.1553-73661553-737410.1371/journal.ppat.1000824https://doaj.org/article/43c867db15814e329b64201206fda0162010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20368966/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The correct organization of single subunits of multi-protein machines in a three dimensional context is critical for their functionality. Type III secretion systems (T3SS) are molecular machines with the capacity to deliver bacterial effector proteins into host cells and are fundamental for the biology of many pathogenic or symbiotic bacteria. A central component of T3SSs is the needle complex, a multiprotein structure that mediates the passage of effector proteins through the bacterial envelope. We have used cryo electron microscopy combined with bacterial genetics, site-specific labeling, mutational analysis, chemical derivatization and high-resolution mass spectrometry to generate an experimentally validated topographic map of a Salmonella typhimurium T3SS needle complex. This study provides insights into the organization of this evolutionary highly conserved nanomachinery and is the basis for further functional analysis.Oliver SchraidtMatthew D LefebreMatthias J BrunnerWolfgang H SchmiedAndreas SchmidtJulia RadicsKarl MechtlerJorge E GalánThomas C MarlovitsPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 4, p e1000824 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Oliver Schraidt Matthew D Lefebre Matthias J Brunner Wolfgang H Schmied Andreas Schmidt Julia Radics Karl Mechtler Jorge E Galán Thomas C Marlovits Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
description	The correct organization of single subunits of multi-protein machines in a three dimensional context is critical for their functionality. Type III secretion systems (T3SS) are molecular machines with the capacity to deliver bacterial effector proteins into host cells and are fundamental for the biology of many pathogenic or symbiotic bacteria. A central component of T3SSs is the needle complex, a multiprotein structure that mediates the passage of effector proteins through the bacterial envelope. We have used cryo electron microscopy combined with bacterial genetics, site-specific labeling, mutational analysis, chemical derivatization and high-resolution mass spectrometry to generate an experimentally validated topographic map of a Salmonella typhimurium T3SS needle complex. This study provides insights into the organization of this evolutionary highly conserved nanomachinery and is the basis for further functional analysis.
format	article
author	Oliver Schraidt Matthew D Lefebre Matthias J Brunner Wolfgang H Schmied Andreas Schmidt Julia Radics Karl Mechtler Jorge E Galán Thomas C Marlovits
author_facet	Oliver Schraidt Matthew D Lefebre Matthias J Brunner Wolfgang H Schmied Andreas Schmidt Julia Radics Karl Mechtler Jorge E Galán Thomas C Marlovits
author_sort	Oliver Schraidt
title	Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
title_short	Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
title_full	Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
title_fullStr	Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
title_full_unstemmed	Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
title_sort	topology and organization of the salmonella typhimurium type iii secretion needle complex components.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/43c867db15814e329b64201206fda016
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Topology and organization of the Salmonella typhimurium type III secretion needle complex components.

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