Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells
Shuying Hu,1 Hanbang Chen,1 Xuefeng Zhou,2 Gang Chen,1 Ke Hu,3 Yi Cheng,1 Lili Wang,1 Feimin Zhang1 1Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 2State Key Laboratory of Bioelectronics,...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/43c88c12fbd647e09773614f38914449 |
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| Sumario: | Shuying Hu,1 Hanbang Chen,1 Xuefeng Zhou,2 Gang Chen,1 Ke Hu,3 Yi Cheng,1 Lili Wang,1 Feimin Zhang1 1Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China; 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; 3Key Laboratory of Clinical and Medical Engineering, Department of Biomedical Engineering, School of Basic Medical Science, Nanjing Medical University, Nanjing 210000, China Introduction: Scaffold structure plays a vital role in cell behaviors. Compared with two-dimensional structure, 3D scaffolds can mimic natural extracellular matrix (ECM) and promote cell–cell and cell–matrix interactions. The combination of osteoconductive scaffolds and osteoinductive growth factors is considered to have synergistic effects on bone regeneration. Materials and methods: In this study, core–shell poly(lactide-co-glycolide) (PLGA)/polycaprolactone (PCL)–BMP-2 (PP–B) fibrous scaffolds were prepared through coaxial electrospinning. Next, we fabricated 3D scaffolds based on PP–B fibers with thermally induced self-agglomeration (TISA) method and compared with conventional PLGA/PCL scaffolds in terms of scaffold morphology and BMP-2 release behaviors. Then, rat adipose-derived stem cells (rADSCs) were seeded on the scaffolds, and the effects on cell proliferation, cell morphology, and osteogenic differentiation of rADSCs were detected. Results: The results demonstrated that 3D scaffold incorporated with BMP-2 significantly increased proliferation and osteogenic differentiation of rADSCs, followed by PP–B group. Conclusion: Our findings indicate that scaffolds with 3D structure and osteoinductive growth factors have great potential in bone tissue engineering. Keywords: BMP-2, core–shell scaffolds, 3D fibrous scaffolds, osteogenic differentiation |
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