A Plausible Proposition of CCL20-Related Mechanism in Fusobacterium nucleatum-Associated Oral Carcinogenesis

Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of Fusobacterium nucleatum in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in Fusobacterium nucl...

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Autores principales:	Devi Prasad Mandal, Neeta Mohanty, Paresh Kumar Behera, Divya Gopinath, Sasmita Panda, Abdulaziz A. Al-Kheraif, Darshan Devang Divakar, Sukumaran Anil, Swagatika Panda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	oral squamous cell carcinoma <i>Fusobacterium nucleatum</i> CCL20 Science Q
Acceso en línea:	https://doaj.org/article/43cdc6676e73475eb69c637df19b79a6
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spelling	oai:doaj.org-article:43cdc6676e73475eb69c637df19b79a62021-11-25T18:11:18ZA Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis10.3390/life111112182075-1729https://doaj.org/article/43cdc6676e73475eb69c637df19b79a62021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1218https://doaj.org/toc/2075-1729Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of <i>Fusobacterium nucleatum</i> in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of <i>Fusobacterium nucleatum</i> was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC tissues. Results: <i>Fusobacterium nucleatum</i> was significantly (<i>p</i> < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of <i>Fusobacterium nucleatum</i> or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (<i>p</i> = 0.053), the CCL20-positive cell population was significantly different (<i>p</i> = 0.034) between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC. Conclusion: <i>Fusobacterium nucleatum</i> is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in <i>Fusobacterium nucleatum</i>-mediated oral carcinogenesis.Devi Prasad MandalNeeta MohantyParesh Kumar BeheraDivya GopinathSasmita PandaAbdulaziz A. Al-KheraifDarshan Devang DivakarSukumaran AnilSwagatika PandaMDPI AGarticleoral squamous cell carcinoma<i>Fusobacterium nucleatum</i>CCL20ScienceQENLife, Vol 11, Iss 1218, p 1218 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oral squamous cell carcinoma <i>Fusobacterium nucleatum</i> CCL20 Science Q
spellingShingle	oral squamous cell carcinoma <i>Fusobacterium nucleatum</i> CCL20 Science Q Devi Prasad Mandal Neeta Mohanty Paresh Kumar Behera Divya Gopinath Sasmita Panda Abdulaziz A. Al-Kheraif Darshan Devang Divakar Sukumaran Anil Swagatika Panda A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
description	Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of <i>Fusobacterium nucleatum</i> in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of <i>Fusobacterium nucleatum</i> was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC tissues. Results: <i>Fusobacterium nucleatum</i> was significantly (<i>p</i> < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of <i>Fusobacterium nucleatum</i> or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (<i>p</i> = 0.053), the CCL20-positive cell population was significantly different (<i>p</i> = 0.034) between <i>Fusobacterium nucleatum</i>-positive and -negative OSCC. Conclusion: <i>Fusobacterium nucleatum</i> is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in <i>Fusobacterium nucleatum</i>-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in <i>Fusobacterium nucleatum</i>-mediated oral carcinogenesis.
format	article
author	Devi Prasad Mandal Neeta Mohanty Paresh Kumar Behera Divya Gopinath Sasmita Panda Abdulaziz A. Al-Kheraif Darshan Devang Divakar Sukumaran Anil Swagatika Panda
author_facet	Devi Prasad Mandal Neeta Mohanty Paresh Kumar Behera Divya Gopinath Sasmita Panda Abdulaziz A. Al-Kheraif Darshan Devang Divakar Sukumaran Anil Swagatika Panda
author_sort	Devi Prasad Mandal
title	A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
title_short	A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
title_full	A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
title_fullStr	A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
title_full_unstemmed	A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis
title_sort	plausible proposition of ccl20-related mechanism in <i>fusobacterium nucleatum</i>-associated oral carcinogenesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/43cdc6676e73475eb69c637df19b79a6
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A Plausible Proposition of CCL20-Related Mechanism in <i>Fusobacterium nucleatum</i>-Associated Oral Carcinogenesis

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