Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit

Purpose The lower urinary tract is believed to be centrally regulated with the involvement of a range of neurotransmitters. The parasympathetic excitatory input to the urinary bladder is suppressed when the serotonergic system is activated, and thereby voiding is blocked. In healthy people, continen...

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Autores principales:	Jae Heon Kim, Young Soo Ahn, Yun Seob Song
Formato:	article
Lenguaje:	EN
Publicado:	Korean Continence Society 2021
Materias:	serotonin hippocampal slices n-methyl-d-aspartate norepinephrine dopamine Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/43ce72e7b80a4b0bae0824013ce34af5
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spelling	oai:doaj.org-article:43ce72e7b80a4b0bae0824013ce34af52021-11-30T04:10:47ZSerotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit2093-47772093-693110.5213/inj.2142348.174https://doaj.org/article/43ce72e7b80a4b0bae0824013ce34af52021-11-01T00:00:00Zhttp://www.einj.org/upload/pdf/inj-2142348-174.pdfhttps://doaj.org/toc/2093-4777https://doaj.org/toc/2093-6931Purpose The lower urinary tract is believed to be centrally regulated with the involvement of a range of neurotransmitters. The parasympathetic excitatory input to the urinary bladder is suppressed when the serotonergic system is activated, and thereby voiding is blocked. In healthy people, continence is usually underpinned by hippocampal formation (circuit 3). In order to advance knowledge of how serotoninergic neurons and additional nerve fibers were correlated, the purpose of the present work was to research how the discharge of serotonin from hippocampal slices was affected by different neurotransmitters in rat models. Methods The adopted procedure involved administration of the central neurotransmitters acetylcholine, norepinephrine, dopamine, N-methyl-D-aspartate (NMDA), gamma-aminobutyric acid (GABA), glycine, and neuropeptide Y as well as monitoring of the alterations in the discharge of [3H]5-hydroxytryptamine (5-HT). Furthermore, to determine whether the effect of the neurotransmitters was influenced by interneuron, tetrodotoxin was also employed. Results Acetylcholine (10-5M) did not alter [3H]5-HT discharge, whereas more 5-HT was discharged from the hippocampal slices of rats under stimulation by norepinephrine (10-5M) as well as dopamine (10-5M) and tetrodotoxin (10-6M) did not inhibit the discharge. By contrast, tetrodotoxin inhibited the discharge of [3H]5-HT that was exacerbated by NMDA (10-4M). Meanwhile, compared to control, GABA (10-5M), glycine (10-5M), or neuropeptide Y (10-6M) did not alter the [³H]5-HT discharge. Conclusions From the research findings, it can be concluded that 5-HT discharge from rat hippocampus is enhanced by norepinephrine and dopamine through direct effect on the 5-HT neuronal terminal. By contrast, 5-HT discharge is intensified by NMDA by activating interneurons.Jae Heon KimYoung Soo AhnYun Seob SongKorean Continence Societyarticleserotoninhippocampal slicesn-methyl-d-aspartatenorepinephrinedopamineDiseases of the genitourinary system. UrologyRC870-923ENInternational Neurourology Journal, Vol 25, Iss Suppl 2, Pp S106-113 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	serotonin hippocampal slices n-methyl-d-aspartate norepinephrine dopamine Diseases of the genitourinary system. Urology RC870-923
spellingShingle	serotonin hippocampal slices n-methyl-d-aspartate norepinephrine dopamine Diseases of the genitourinary system. Urology RC870-923 Jae Heon Kim Young Soo Ahn Yun Seob Song Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit
description	Purpose The lower urinary tract is believed to be centrally regulated with the involvement of a range of neurotransmitters. The parasympathetic excitatory input to the urinary bladder is suppressed when the serotonergic system is activated, and thereby voiding is blocked. In healthy people, continence is usually underpinned by hippocampal formation (circuit 3). In order to advance knowledge of how serotoninergic neurons and additional nerve fibers were correlated, the purpose of the present work was to research how the discharge of serotonin from hippocampal slices was affected by different neurotransmitters in rat models. Methods The adopted procedure involved administration of the central neurotransmitters acetylcholine, norepinephrine, dopamine, N-methyl-D-aspartate (NMDA), gamma-aminobutyric acid (GABA), glycine, and neuropeptide Y as well as monitoring of the alterations in the discharge of [3H]5-hydroxytryptamine (5-HT). Furthermore, to determine whether the effect of the neurotransmitters was influenced by interneuron, tetrodotoxin was also employed. Results Acetylcholine (10-5M) did not alter [3H]5-HT discharge, whereas more 5-HT was discharged from the hippocampal slices of rats under stimulation by norepinephrine (10-5M) as well as dopamine (10-5M) and tetrodotoxin (10-6M) did not inhibit the discharge. By contrast, tetrodotoxin inhibited the discharge of [3H]5-HT that was exacerbated by NMDA (10-4M). Meanwhile, compared to control, GABA (10-5M), glycine (10-5M), or neuropeptide Y (10-6M) did not alter the [³H]5-HT discharge. Conclusions From the research findings, it can be concluded that 5-HT discharge from rat hippocampus is enhanced by norepinephrine and dopamine through direct effect on the 5-HT neuronal terminal. By contrast, 5-HT discharge is intensified by NMDA by activating interneurons.
format	article
author	Jae Heon Kim Young Soo Ahn Yun Seob Song
author_facet	Jae Heon Kim Young Soo Ahn Yun Seob Song
author_sort	Jae Heon Kim
title	Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit
title_short	Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit
title_full	Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit
title_fullStr	Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit
title_full_unstemmed	Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit
title_sort	serotonin discharge regulation by additional neurotransmitters of rat hippocampus associated with the continence central circuit
publisher	Korean Continence Society
publishDate	2021
url	https://doaj.org/article/43ce72e7b80a4b0bae0824013ce34af5
work_keys_str_mv	AT jaeheonkim serotonindischargeregulationbyadditionalneurotransmittersofrathippocampusassociatedwiththecontinencecentralcircuit AT youngsooahn serotonindischargeregulationbyadditionalneurotransmittersofrathippocampusassociatedwiththecontinencecentralcircuit AT yunseobsong serotonindischargeregulationbyadditionalneurotransmittersofrathippocampusassociatedwiththecontinencecentralcircuit
_version_	1718406859514183680

Serotonin Discharge Regulation by Additional Neurotransmitters of Rat Hippocampus Associated With the Continence Central Circuit

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