Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Sisi Zheng, Li Luo, Ruonan Bo, Zhenguang Liu, Jie Xing, Yale Niu, Yuanliang Hu, Jiaguo Liu, Deyun Wang Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of China Abstract: This study aimed to...

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Autores principales:	Zheng S, Luo L, Bo R, Liu Z, Xing J, Niu Y, Hu Y, Liu J, Wang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	PHYP optimal preparation condition RSM in vitro study Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/43d346497df7476aac7155abad8259e1
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spelling	oai:doaj.org-article:43d346497df7476aac7155abad8259e12021-12-02T05:26:33ZEvaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study1178-2013https://doaj.org/article/43d346497df7476aac7155abad8259e12016-09-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-optimum-conditions-for-pachyman-encapsulated-in-polydl-l-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sisi Zheng, Li Luo, Ruonan Bo, Zhenguang Liu, Jie Xing, Yale Niu, Yuanliang Hu, Jiaguo Liu, Deyun Wang Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of China Abstract: This study aimed to optimize the preparation conditions of pachyman (PHY)-loaded poly(D,L-lactic acid) (PLA) (PHYP) nanospheres by response surface methodology, explore their characteristics, and assess their effects on splenic lymphocytes. Double emulsion solvent evaporation was used to synthesize PHYP nanospheres, and the optimal preparation conditions were identified as a concentration of poloxamer 188 (F68) (w/v) of 0.33%, a concentration of PLA of 30 mg/mL, and a ratio of PLA to drug (w/w) of 10.25:1 required to reach the highest encapsulation efficiency, which was calculated to be 59.10%. PHYP had a spherical shape with a smooth surface and uniform size and an evident effect of sustained release and relative stability. Splenic lymphocytes are crucial and multifunctional cells in the immune system, and their immunological properties could be enhanced significantly by PHYP treatment. This study confirmed that PHY encapsulated in PLA nanospheres had comparatively steady properties and exerted obvious immune enhancement. Keywords: PHYP, optimal preparation condition, RSM, in vitro studyZheng SLuo LBo RLiu ZXing JNiu YHu YLiu JWang DDove Medical PressarticlePHYPoptimal preparation conditionRSMin vitro studyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4891-4904 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	PHYP optimal preparation condition RSM in vitro study Medicine (General) R5-920
spellingShingle	PHYP optimal preparation condition RSM in vitro study Medicine (General) R5-920 Zheng S Luo L Bo R Liu Z Xing J Niu Y Hu Y Liu J Wang D Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
description	Sisi Zheng, Li Luo, Ruonan Bo, Zhenguang Liu, Jie Xing, Yale Niu, Yuanliang Hu, Jiaguo Liu, Deyun Wang Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of China Abstract: This study aimed to optimize the preparation conditions of pachyman (PHY)-loaded poly(D,L-lactic acid) (PLA) (PHYP) nanospheres by response surface methodology, explore their characteristics, and assess their effects on splenic lymphocytes. Double emulsion solvent evaporation was used to synthesize PHYP nanospheres, and the optimal preparation conditions were identified as a concentration of poloxamer 188 (F68) (w/v) of 0.33%, a concentration of PLA of 30 mg/mL, and a ratio of PLA to drug (w/w) of 10.25:1 required to reach the highest encapsulation efficiency, which was calculated to be 59.10%. PHYP had a spherical shape with a smooth surface and uniform size and an evident effect of sustained release and relative stability. Splenic lymphocytes are crucial and multifunctional cells in the immune system, and their immunological properties could be enhanced significantly by PHYP treatment. This study confirmed that PHY encapsulated in PLA nanospheres had comparatively steady properties and exerted obvious immune enhancement. Keywords: PHYP, optimal preparation condition, RSM, in vitro study
format	article
author	Zheng S Luo L Bo R Liu Z Xing J Niu Y Hu Y Liu J Wang D
author_facet	Zheng S Luo L Bo R Liu Z Xing J Niu Y Hu Y Liu J Wang D
author_sort	Zheng S
title	Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
title_short	Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
title_full	Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
title_fullStr	Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
title_full_unstemmed	Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
title_sort	evaluation of optimum conditions for pachyman encapsulated in poly(d,l-lactic acid) nanospheres by response surface methodology and results of a related in vitro study
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/43d346497df7476aac7155abad8259e1
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Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

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